1a.Objectives (from AD-416):
The objective of this research is to identify bacterial genes required for tick colonization.
1b.Approach (from AD-416):
A negative-selection screen will be done using a fully characterized, Francisella novicida, two-allele mutant library.
This work relates to objective 1 of parent project by provision of pathogen genome data concerning, the identification of genes required for transmission of tick borne pathogens. The long-term goal of this project is to identify genes that are required for tick transmission by doing negative selection screens using a mutant library from the tick transmitted bacteria, Francisella novicida. Approximately 200 F. novicida mutants have been tested, and four genes have been identified that are involved in tick colonization. One of these genes encodes for an outer membrane protein of unknown function. The other three genes are involved in a variety of cellular functions including iron acquisition, regulation of gene expression and cell wall biosynthesis. The iron acquisition pathway is of particular interest because iron metabolism is also likely important in tick colonization of A. marginale.