1a.Objectives (from AD-416):
Evaluate new combinations of fatty acid profiles with the high oleic acid trait. This project will produce soybean germplasm in a high oleic acid background with additional oil profile traits to determine the effect of different combinations of genes to produce desirable fatty acid profiles in the oil. Targets include high oleic acid (HO) plus low palmitic acid, HO plus low palmitic acid and low linolenic acid, HO plus high stearic acid, and HO plus increased bio-available tocopherol content. The genes controlling the traits will be merged using the latest molecular marker technology, and germplasm will be produced and tested in an appropriate field environment for the fatty acid profiles of the seeds.
1b.Approach (from AD-416):
Molecular genetics and plant breeding experiments will be conducted to combine desired mutant alleles controlling the accumulation of different fatty acids in the seed oil. Different crosses will be made between parental lines containing the two genes for the high oleic acid trait and parental lines containing other fatty acid profile traits, including low palmitic acid and elevated stearic acid. Genotyping selections with existing or newly developed molecular marker assays will be performed on the F1 and F2 progeny. Standard population development will be used to advance selected lines to obtain materials for phenotypic evaluation. Field tests will be set up to compare different combinations of alleles important for the seed oil fatty acid profiles to evaluate the effect of each of the alleles working in combination. Laboratory, field, and winter nursery are all components of this research.
The objective of this research was to determine the fatty acid profile of different gene combinations in a high oleic acid oil background. Mutant alleles of genes controlling the accumulation of palmitic acid, stearic acid, and linoleic and linolenic acid were combined by molecular selection in soybean lines containing the high oleic acid trait. A two location field trial was used to produce seed for the evaluation of fatty acid profile and analyses.