DETERMINING THE BREEDING POTENTIAL OF NEAR-ELS GERMPLASM
Location: Coastal Plain Soil, Water and Plant Conservation Research
Project Number: 6657-21000-006-10
Start Date: Jan 01, 2012
End Date: Dec 31, 2012
Develop Upland cotton breeding populations using near-extra-long staple (ELS) germplasm.
In 2008, thirteen near-extra-long staple (ELS) accessions were obtained from the USDA-ARS National Cotton Collection in College Station, Texas. Over two years, these accessions were grown in the field, self-pollinated, purified, and seed increased. Eleven of the near-ELS accessions were confirmed to possess near-ELS fiber properties and will be used in two mating schemes to generate segregating breeding populations.
In 2011, the eleven near-ELS accessions were each crossed to 5-6 elite Upland cotton germplasm lines using a topcross mating design. In September 2011, the resulting F1 crosses were sent to the Cotton Winter Nursery for seed increase. In 2012, the F2 hybrids and parental lines will be evaluated in replicated trials in 1-3 environments across South Carolina and North Carolina depending on seed availability. Yield and yield component data will be collected. Prior to harvest, a boll sample will be hand harvested and used to calculate lint percent and yield components. The boll sample will also provide a fiber sample for measuring HVI fiber properties. Using an additive-dominance mixed model, variance components and estimates of general and specific combining ability will be calculated for each cross combination. Seed from selected families will be advanced and integrated into the progeny row nursery of the breeding program.
Also in 2012, a half-diallel mating scheme will be initiated among the same eleven near-ELS accessions. F1 crosses will be increased at the Cotton Winter Nursery. In 2013, the F2 hybrids and parental lines will be evaluated in replicated trials in 1-3 environments across South Carolina and North Carolina depending on seed availability. Yield, yield components, and HVI fiber properties will be collected and statistically analyzed. Analyses will focus primarily on determining the extent of genetic variation for fiber properties among near-ELS x near-ELS matings. Seed from selected near-ELS x near-ELS families will be advanced and integrated into the progeny row nursery of the breeding program.