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United States Department of Agriculture

Agricultural Research Service

Research Project: Novel Real-Time PCR-Based Surveillance Systems for Candidatus Liberibacter Species, Bacteria Associated with Citrus Huanglongbing Project Number: 5302-22000-010-06
Project Type: Trust

Start Date: Dec 01, 2010
End Date: Nov 30, 2012

Objective:
1. To develop ultra sensitive Taq-Man PCR diagnostic system for improved detection of Ca. Liberibacter asiaticus. 2. To design a Ca. Liberibacter genus-specific real-time PCR technology for general detection of Candidatus Liberibacter species.

Approach:
1. Cloning, sequencing and characterizing genomic sequences of citrus Candidatus Liberibacter species using novel molecular technique. 2. Design and validate sensitivity and specificity of diagnostic systems.

Last Modified: 12/22/2014
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