1a.Objectives (from AD-416)
Produce F3 populations of Northwest-adapted barleys crossed with sources of genes for resistance to the Ug99 stem rust pathogen.
1b.Approach (from AD-416)
Make crosses between Ug99-resistant barley lines and various market types of barley adapted to Pacific Northwest production. The Ug99 resistant lines will be from ICARDA/CIMMYT material previously tested by Brian Steffenson at University of Minnesota, and the locally-adapted lines will include winter barley of malting, food and forage types. Conduct field evaluations of F2 populations for agronomic traits including cold tolerance and resistance to local diseases, then advance to the F3 generation for further testing. The materials resulting from this SCA will be available for further development and testing to select materials that are locally adapted and have resistance to Ug99. This SCA supports research conducted under National Program (NP) Number303, Plant Diseases. The research is conducted as part of ARS research project 5358-22000-035-00D, augmented by the FY2010 Program Increase for Research to Strengthen Grain Disease Research to Reduce World Hunger.
Seventeen crosses were made between donors of UG99 resistance (identified by the international UG99 screening consortium trials in Kenya) and representatives of the principal classes of winter and facultative barley adapted to the Pacific Northwest of the USA. F1s were planted in the field at Corvallis in the Fall of 2010 and were also advanced to the F2 generation in the greenhouse. Seven selected F2 populations were sent to South Africa for phenotypic selection in the Spring of 2011. Resistant F2 plants will be selected and the F3 progeny integrated into the ongoing OSU breeding program.
Thirty accessions representing elite winter and facultative growth habit from the OSU Program were sent for UG99 phenotyping in Kenya via the USDA-ARS nursery. When the genome coordinates of UG99 resistance loci are determined, we will be positioned to implement marker assisted selection.
The project was monitored through site visits, meetings, email correspondence and review.