1a.Objectives (from AD-416):
Novel phenotypic variation will be identified and genetically characterized and mapped on the cranberry genome. A comprehensive molecular marker system for cranberry will be developed using available 454 sequence data and genotyping by sequence technology.
1b.Approach (from AD-416):
Microsatellite (=2400) loci will be mined and primers will be tested using the parentals for several available mapping populations. A set of 500 microsatellite loci will be used for linkage map development using 179 individuals mapping population from the New Jersey. The map will incorporate trait information regarding yield and quality characteristics and field fruit-rot resistance. The SSR cranberry linkage map will be enhanced with SNP markers derived from genotyping-by-sequencing.
Cranberry accessions and progenies in the Chatsworth, New Jersey collection are being phenotypically evaluated per plot for yield, fruit size, fruit set, vegetative vigor, fruit rot resistance, and polyphenolics. Additionally, 10 individual uprights per plot are also being evaluated to determine upright length, dry weight of leaves, total number of pedicels (with and without fruit), number of berries, number of aborted flowers and berries, and total and berry weight. The biggest berry for each upright was measured (in centimeters) for length, width, weight, and calyx diameter and seeds will be counted and weighed for each fruit. Cranberry fruit quality was assessed based on calyx, shape, skin, and seed characters. The information will be incorporated in a high-resolution molecular map for cranberry.
This research relates to the initiation of genomic analysis of cranberry and its relatives that will provide molecular markers to be tied to field variation.