PHYTOESTROGENIC EFFECTS OF ELICITOR INDUCED ISOFLAVONOIDS IN LEGUMES
Project Number: 6435-53000-002-00
Start Date: Nov 23, 2009
End Date: Mar 31, 2011
1. Induce, purify, and characterize isoflavonoids in soybean seed, root, plant, and cell culture induced with food grade elicitors including fungi.
2. Determine anticancer, hormonal, and antioxidant activities of individual, and combinations of, induced isoflavonoids with in vitro bioassays. Effects of phytoalexins using in vitro cell systems for PPAR transcriptional activity, adipocyte differentiation, and obesity related gene expression will be tested. In vivo gene expression from tissue samples from ongoing experiments will be evaluated. Computer modeling of potential phytoalexin structures for PPAR agonist activity will be performed.
3. Determine hormonal and anticancer effects of individual and combinations of induced isoflavonoids with in vivo animal model systems.
4. Develop methods for the organic syntheses of the glyceollins I, II, and III. Also, precursors and synthetic analogues of the glyceollins will be produced and characterized for beneficial hormonal activities. Computer modeling methods will be developed to assist in the development of structure-activity relationships that point toward the most active forms of the molecule.
The long-term objective of this project is to determine the health effects of induced isoflavones in both in vitro and in vivo model systems. Preliminary data indicate that the induced isoflavone glyceollins from soybean have anticancer activity against several different forms of cancer. This project will focus on natural induced isoflavones and foods containing these compounds. Foods containing these induced isoflavones called “activated foods” will be developed and tested for beneficial health promoting properties. Although the focus of this project is on natural compounds, synthetic compounds based on these natural compounds will also be utilized for the prevention of cancer and safety testing.