1a.Objectives (from AD-416)
1. Different sampling techniques will be determined for microbial pathogens in edible catfish products: swab, whole fillets sampling, ice if finfish in direct contact, and drip liquid if frozen then thawed.
2. Pathogen test methods (selective agar or test kits) for Salmonella, Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcal aureus will be compared to determine influence of catfish components (interfering histamine) and time to obtain results.
3. Confirmation will be done on positive samples to verify presence of human pathogen.
4. Data obtained delivered to FSIS for retail catfish inspection and extension personal to develop literature for proper handling of raw catfish.
1b.Approach (from AD-416)
Catfish to be purchased at various retail stores and microbial counts done. Bacteria identification done on the isolates to determine if human pathogens are present.
This information will be used by FSIS to develop a sampling program.
This report pertains to funding provided by USDA Food Safety Inspection Service, to the Cheyney University (pass through funding) to address the research needs of the FSIS Office of Catfish Inspection Program. Transfer of catfish inspection duties from the U.S. FDA to the USDA FSIS was mandated by the 2008 Farm Bill. The funds were disbursed to Cheyney University in January 2010. Equipment, as outlined in the grant proposal, has been purchased. The research to address the microbiological quality and safety of retail catfish was initiated by Cheyney scientists in January, 2010. Three minority students and technician worked at the ARS facility. Three research coordination meetings have been held since December, 2009. Preliminary results of the research will be presented at a Federal Catfish Research Meeting in August, 2010.
Progress has been monitored through meetings, conference calls and site visits.
Additional information is provided in the annual report for 1935-42000-054-05G.