1a.Objectives (from AD-416)
The objective of this cooperative research project is to develop multiplex peptide nucleotide acid (PNA) chip and immune biomarkers associated with food-borne pathogens.
1b.Approach (from AD-416)
NVRQS will develop multiplex PNA chip by identifying SNP of ffod-borne pathogens and validate PNA chip for diagnosis and detection of Clostridium, Salmonella and Campylobacter.
ARS will develop disease challenge models to evaluate vaccines against poultry food-poisoning pathogen, develop and optimize real-time RT-PCR for detection of C. septicum and C. perfringens, develop ELISA for chicken cytokine detection to identify immune biomarkers associated with C. perfringens and C. septicum and evaluate potential vaccine candidates of Clostricium proteins.
Major progress was made in identifying clostridium proteins which are recognized by hyperimmune serum from field clostridium outbreaks (C. perfringens) using proteomics. Serological analysis of clinically sick birds confirmed that they had high antibody titers against these antigens. Progress is being made in cloning the immunogenic genes of C. perfringens that can lead to the identification of potential vaccines to reduce clostridial infection. Work progress is monitored by regular teleconferences, meetings and formal written progress reports.