2009 Annual Report
The project is related to objective 4 of the in-house project: Use molecular epidemiologic techniques and viral genomics to understand virus transmission and spread of avian influenza outbreaks in poultry and wild birds.
Some strains of animal influenza virus have been zoonotic agents necessitating joint research between veterinary medicine and human health to solve the problems including the H5N1 high pathogenicity avian influenza (HPAI) virus. During 2009, simple methods were investigated that would reduce human exposure to H5N1 viruses in infected poultry during home slaughter process. A new assay was developed and validated for virus neutralization in embryonating chicken eggs to assess the amino acid changes found between the H5s of a vaccine seed and its drift variant viruses in their influences on the antigenicity of this protein. Hemagglutinin-specific antisera were used to neutralize reverse genetics-produced viruses that were identical except for their HA genes. Some differences were seen in the virus neutralization titers between the wild type and mutant viruses. Further studies are underway to gain further information.
Monitoring: The Principal Investigator had regular email and telephone conversations with the cooperator on research progress.