Skip to main content
ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Insect Behavior and Biocontrol Research » People & Locations » Paul Shirk
Paul D Shirk
Volunteer


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Gene editing of the ABC Transporter/White locus using CRISPR/Cas9 mutagenesis in the Indian Meal Moth - (Peer Reviewed Journal)
Shirk, B.D., Shirk, P.D., Furlong, R.B., Scully, E.D., Wu, K., Siegfried, B.D. 2022. Gene editing of the ABC Transporter/White locus using CRISPR/Cas9-mediated mutagenesis in the Indian meal moth. Journal of Insect Physiology. 145:104471. https://doi.org/10.1016/j.jinsphys.2022.104471.
Effect of Ephestia kuehniella (Lepidoptera: Pyralidae) larval diet on egg quality and parasitism by Trichogramma brassicae (Hymenoptera: Trichogrammatidae) - (Peer Reviewed Journal)
Moghaddasi, Y., Ashouri, A., Bandani, A., Leppla, N.C., Shirk, P.D. 2019. Effect of Ephestia kuehniella (Lepidoptera: Pyralidae) larval diet on egg quality and parasitism by Trichogramma brassicae (Hymenoptera: Trichogrammatidae). Journal of Insect Science. 19(4):1-7. https://doi.org/10.1093/jisesa/iez076.
CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in fall armyworm moth (Spodoptera frugiperda) - (Peer Reviewed Journal)
Wu, K., Shirk, P.D., Taylor, C.E., Furlong, R.B., Shirk, B.D., Pinheiro, D.H., Siegfried, B.D. 2018. CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in fall armyworm moth (Spodoptera frugiperda). PLoS One. 13(2):e0208647. https://doi.org/10.1371/journal.pone.0208647.
Mortality effects of three bacterial pathogens and Beauveria bassiana when topically applied or injected into house flies (Diptera: Muscidae) - (Peer Reviewed Journal)
Johnson, D.M., Weeks, E.N., Lovullo, E.D., Shirk, P.D., Geden, C.J. 2018. Mortality effects of three bacterial pathogens and Beauveria bassiana when topically applied or injected into house flies (Diptera: Muscidae). Journal of Medical Entomology. 56(3):774-783. https://doi.org/10.1093/jme/tjy218.
Functional characterization of transcriptional regulatory elements of three highly expressed constitutive genes in the jewel wasp, Nasonia vitripennis - (Peer Reviewed Journal)
Shirk, P.D., Furlong, R.B., Dolan, A., Werren, J.H. 2017. Functional characterization of transcriptional regulatory elements of three highly expressed constitutive genes in the jewel wasp, Nasonia vitripennis. Insect Molecular Biology. 26(6):743-750. https://doi.org/10.1111/imb.12333.
Induction of Vermillion in Pyralid moths using CRISPR mutagenesis - (Abstract Only)
A feeding protocol for delivery of agents to assess development in Varroa mites - (Peer Reviewed Journal)
Cabrera, A.R., Shirk, P.D., Teal, P.T. 2017. A feeding protocol for delivery of agents to assess development in Varroa mites. PLoS One. 12(4):e0176097. https://doi.org/10.1371/journal.pone.0176097.
Co-acquisition of pesticide resitance within the hive - (Abstract Only)
Insect cell transformation vectors that support high level expression and promoter assessment in insect cell culture - (Peer Reviewed Journal)
Shirk, P.D., Furlong, R.B. 2016. Insect cell transformation vectors that support high level expression and promoter assessment in insect cell culture. Plasmid Journal. 83:12-19. Available: http://dx.doi.org/10.1016/j.plasmid.2016.01.001
Impacts of extended laboratory rearing on female fitness in Florida colonies of the parasitoid spalangia cameroni (Hymenoptera: Pteromalidae) with an analysis of wolbachia strains - (Peer Reviewed Journal)
Machtinger, E.T., Geden, C.J., Lovullo, E.D., Shirk, P.D. 2015. Impacts of extended laboratory rearing on female fitness in Florida colonies of the parasitoid spalangia cameroni (Hymenoptera: Pteromalidae) with an analysis of wolbachia strains. Annals of the Entomological Society of America. 109(2):176-182.
Unique synteny and alternate splicing of the chitin synthases in closely related heliothine moths - (Peer Reviewed Journal)
Shirk, P.D., Perera, O.P., Shelby, K., Furlong, R.B., LoVullo, E.D., Popham, H.J. 2015. Unique synteny and alternate splicing of the chitin synthases in closely related heliothine moths. Gene. 574(1):121-139.
The lipid biosynthesis hole in the rickettsiales - (Abstract Only)
Isolation, characterization, and expression analyses of ecdysone receptor 1, ecdysone receptor 2 and ultraspiracle genes in varroa destructor mite - ()
Li, Q., Cabrera Cordon, A.R., Teal, P.E., Shirk, P.D. 2015. Isolation, characterization, and expression analyses of ecdysone receptor 1, ecdysone receptor 2 and ultraspiracle genes in varroa destructor mite. Genbank. KR230042-46,KR363132.
Ecdysteroid biosynthesis in varroa mites: identification of halloween genes from the biosynthetic pathway and their regulation during reproduction - (Abstract Only)
Ecdysteroid biosynthesis in varroa mites: identification of halloween genes from the biosynthetic pathway - (Abstract Only)
Three halloween genes from the varroa mite, varroa destructor (Anderson & Trueman) and their expression during reproduction - (Peer Reviewed Journal)
Cabrera, A.R., Shirk, P.D., Evans, J.D., Hung, K., Sims, J.W., Alborn, H.T., Teal, P.E. 2014. Three halloween genes from the varroa mite, varroa destructor (Anderson & Trueman) and their expression during reproduction. Insect Molecular Biology. doi: 10.1111/imb.12155.
Examining the role of foraging and malvolio in host-finding behavior in the honey bee parasite, Varroa destructor (Anderson & Trueman) - (Peer Reviewed Journal)
Cabrera, A.R., Shirk, P.D., Grozinger, C.M., Evans, J.D., Teal, P.E. 2013. Examining the role of foraging and malvolio in host-finding behavior in the honey bee parasite, Varroa destructor (Anderson & Trueman). Archives of Insect Biochemistry and Physiology. 85(2):61-75.
Discovery and molecular characterization of an ambisense densovirus from South American populations of Solenopsis invicta - (Peer Reviewed Journal)
Valles, S.M., Shoemaker, D.D., Wurm, Y., Strong, C.A., Varone, L., Becnel, J.J., Shirk, P.D. 2013. Discovery and molecular characterization of an ambisense densovirus from South American populations of Solenopsis invicta . Biological Control. 67:431-439.
Identification of three Halloween genes from the varroa mite and their expression during brood cell invasion - (Abstract Only)
European corn borer (Ostrinia nubilalis) induced defenses in maize enhance susceptibility in maize - (Peer Reviewed Journal)
Dafoe, N.J., Thomas, J.D., Shirk, P.D., Legaspi, M.E., Vaughan, M.M., Huffaker, A., Teal, P.E., Schmelz, E.A. 2013. European corn borer (Ostrinia nubilalis) induced defenses in maize enhance susceptibility in maize. PLoS One. 8(9):1-18.
Identification and molecular cloning of three Halloween genes in the varroa mite, Varroa destructor (Anderson & Trueman) (Acari: Varroidae) - (Abstract Only)
Genomic organization and reproductive regulation of a carrier/storage protein in the Varroa Mite, Varroa destructor (Anderson & Trueman) - (Peer Reviewed Journal)
Cabrera Cordon, A.R., Shirk, P.D., Duehl, A.J., Donohue, K.V., Grozinger, C.M., Evans, J.D., Teal, P.E. 2013. Genomic organization and reproductive regulation of a carrier/storage protein in the Varroa Mite, Varroa destructor (Anderson & Trueman). Insect Molecular Biology. 22(5):505-522.
Closely related Wolbachia (Rickettsiales:Rickettsiaceae) recovered from different genera of Mexican Thelytokous figitidae (Hymenoptera) - (Peer Reviewed Journal)
Davies, A., Sivinski, J.M., Shirk, P.D., Aluja, M. 2013. Closely related Wolbachia (Rickettsiales:Rickettsiaceae) recovered from different genera of Mexican Thelytokous figitidae (Hymenoptera). Florida Entomologist. 96(2):649-653.
Variable induction of vitellogenin genes in the varroa mite, Varroa destructor (Anderson & Trueman) by the honeybee, Apis mellifera L, host and its environment - (Peer Reviewed Journal)
Cabrera Cordon, A.R., Shirk, P.D., Duehl, A.J., Evans, J.D., Teal, P.E. 2013. Variable induction of vitellogenin genes in the varroa mite, Varroa destructor (Anderson & Trueman) by the honeybee, Apis mellifera L, host and its environment. Insect Molecular Biology. 22(1):88-103.
Plant responses to hidden herbivores: European corn borer (ECB; Ostrinia nubilalis) attack on maize induces both defense and susceptibility - (Abstract Only)
Predator-Prey Relationships on Apiaceae at an Organic Farm - (Peer Reviewed Journal)
Shirk, P.D., Shapiro, J.P., Reitz, S.R., Gruters Thomas, J.M., Koenig, R.L., Hay-Roe, M.M., Buss, L.J. 2012. Predator-Prey Relationships on Apiaceae at an Organic Farm. Journal of Environmental Entomology. 41(3):487-496.
Cloning and submission to the Natural Center for Biotechnology Information (NCBI) database of two complete vitellogenin genes from the varroa mite, Varroa destructor - ()
Cabrera Cordon, A.R., Shirk, P.D., Evans, J.D., Teal, P.E. 2012. Cloning and submission to the Natural Center for Biotechnology Information (NCBI) database of two complete vitellogenin genes from the varroa mite, Varroa destructor. Genbank. JQ974976-JQ974977.
Mass-rearing of a tropical minute pirate bug, Orius Pumilio (Hemiptera:Anthocoridae) - (Peer Reviewed Journal)
Gruters Thomas, J.M., Shirk, P.D., Shapiro, J.P., Allan, S.A. 2012. Mass-rearing of a tropical minute pirate bug, Orius Pumilio (Hemiptera:Anthocoridae). Florida Entomologist. 95(1):199-201.
Larval development of Diaphorencyrtus aligarhensis (Hymenoptera: Encyrtidae) an endoparasitoid of Diaphorina citri (Homoptera: Psyllidae). - (Peer Reviewed Journal)
Rohrig, E.A., Shirk, P.D., Hall, D.G., Stansly, P.A. 2011. Larval development of Diaphorencyrtus aligarhensis (Hymenoptera: Encyrtidae) an endoparasitoid of Diaphorina citri (Homoptera: Psyllidae).. Annals of the Entomological Society of America. 104:50-58.
Ovarian development in the predaceous minute pirate bug Orius pumilio: relationship to diet and mating - (Peer Reviewed Journal)
Shapiro, J.P., Shirk, P.D. 2010. Ovarian development in the predaceous minute pirate bug Orius pumilio: relationship to diet and mating. Annals of the Entomological Society of America. 103(6):971-978.
Parthenogenesis induction by Wolbachia in parasitoids - (Abstract Only)
Feeding and Mating are required for Ovarian Development and Egg Production in the Predaceous Minute Pirate Bug Orius pumilio - (Abstract Only)
Shirk, P.D., Shapiro, J.P. 2010. Feeding and Mating are required for Ovarian Development and Egg Production in the Predaceous Minute Pirate Bug Orius pumilio. Developmental Biology.344:A124-No.3562010.
Identity of two sympatric species of Orius (Heteroptera: Anthocoridae) - (Peer Reviewed Journal)
Shapiro, J.P., Shirk, P.D., Kelley, K., Lewis, T.M., Horton, D.R. 2010. Identity of two sympatric species of Orius (Heteroptera: Anthocoridae). Journal of Insect Science. 10:189.
Species specific ITS-1 DNA Sequences for the identification of Orius insidiosus, Orius pumilio and Orius tristicolor. - (Peer Reviewed Journal)
Shirk, P.D., Furlong, R.B., Shapiro, J.P. 2009. Species specific ITS-1 DNA Sequences for the identification of Orius insidiosus, Orius pumilio and Orius tristicolor.. National Center for Biotechnology Information (NCBI).Public Database. Acces. No. GU214726-GU214728.
Single inverted terminal repeats of the Junonia coenia Densovirus promotes somatic chromosomal integration of vector plasmids in insect cells and supports high efficiency expression - (Abstract Only)
N/A
Sympatry of Orius insidiosus and O. pumilio (Hemiptera:Anthocoridae) in north central Florida - (Peer Reviewed Journal)
Shapiro, J.P., Shirk, P.D., Reitz, S.R., Koenig, R. 2009. Sympatry of Orius insidiosus and O. pumilio (Hemiptera: Anthocoridae) in North Central Florida. Florida Entomologist. 92:362-366.
Nutritional manipulation of adult female Orius pumilio (Hemiptera: Anthocoridae) enhances initial predatory performance - (Peer Reviewed Journal)
Shapiro, J.P., Reitz, S.R., Shirk, P.D. 2009. Nutritional manipulation of adult female Orius pumilio (Hemiptera: Anthocoridae) enhances initial predatory performance. Journal of Economic Entomology. 102:500-506.
Vitellogenesis - ()
Shirk, P.D. 2008. Vitellogenesis. In: Capinera, John L., editor. Encyclopedia of Entomology. 2nd edition. Dordrecht, The Netherlands: Springer. p. 93-97.
Care and feeding of Orius insidiosus (Hemiptera: Anthocoridae): Assessing the impact of diet on predation following adult female eclosion - (Abstract Only)
JUNONIA COENIA DENSOVIRUS DERIVED VECTORS OFFER NEW AVENUES FOR EXPLORATION AND EXAMINATION OF POLYDNAVIRUS (PDV) GENE FUNCTION IN VIVO - (Abstract Only)
Nutrition for optimal predatory performance of adult female Orius insidiosus - (Abstract Only)
Shapiro, J.P., Reitz, S.R., Shirk, P.D., Ferkovich, S.M. 2007. Nutrition for optimal predatory performance of adult female Orius insidiosus. Florida Entomological Society Annual Meeting.
Regulation of Junonia coenia Densovirus P9 Promoter Expression - (Peer Reviewed Journal)
Shirk, P.D., Bossin, H., Furlong, R.B., Gillett, J.L. 2007. Regulation of Junonia coenia Densovirus P9 Promoter Expression. Insect Molecular Biology.
Regulation of Junonia coenia densovirus P9 promotor expression - (Peer Reviewed Journal)
Shirk, P.D., Bossin, H., Furlong, R.B., Gillett, J. 2007. Regulation of Junonia coenia densovirus P9 promotor expression. Insect Molecular Biology. 16:623-633.
FUNCTIONALITY OF JCDNV-DERIVED SOMATIC TRANSFORMATION VECTORS IN INSECTS AND THE ROLE OF VIRAL ENHANCER SEQUENCES - (Proceedings)
Shirk, P.D., Gillett, J., Bossin, H. 2006. Functionality of JCDNV-Derived Somatic transformation vectors in insects and the role of viral enhancer sequences. Journal of Insect Science. 6:46.
SOMATIC TRANSFORMATION EFFICIENCIES AND EXPRESSION PATTERNS USING THE JCDNV AND PIGGYBAC TRANSPOSON GENE VECTORS IN INSECTS - (Peer Reviewed Journal)
Bossin, H., Furlong, R.B., Gillett, J.L., Bergoin, M., Shirk, P.D. 2007. Somatic transformation efficiencies and expression patterns using the JcDNV and piggyBac transposon gene vectors in insects. Insect Molecular Biology. 16:37-47.
DEVELOPMENT OF THE LARVAL OVARY IN THE MOTH, PLODIA INTERPUNCTELLA - (Peer Reviewed Journal)
Beckemeyer, E.F., Shirk, P.D. 2004. Development of the larval ovary in the moth, Plodia interpunctella. Journal of Insect Physiology. 50:1045-1051.
VITELLOGENESIS AND POST-VITELLOGENIC MATURATION OF THE INSECT OVARIAN FOLLICLE - ()
Swevers, L., Raikhel, A.S., Sappington, T.W., Shirk, P.D., Iatrou, K. 2005. Vitellogenesis and post-vitellogenic maturation of the insect ovarian follicle. In: Gilbert, L.I., Iatrou, K., Gill, S.S., editors. Comprehensive Molecular Insect Science. Volume 1. New York, NY: Elsevier Pergamon. p. 87-155.
DELETION OF DENSOVIRAL SEQUENCES REDUCES THE EFFICIENCY OF SOMATIC INTEGRATION OF JCDNV-DERIVED PLASMIDS IN INSECTS. - (Proceedings)
SHIRK, P.D., FURLONG, R.B., BOSSIN, H. DELETION OF DENSOVIRAL SEQUENCES REDUCES THE EFFICIENCY OF SOMATIC INTEGRATION OF JCDNV-DERIVED PLASMIDS IN INSECTS. MEETING ABSTRACT. 2003. www.insectscience.org/3.36
FAT BODY PRODUCED YOLK PROTEIN GENES IN HYPHANTRIA CUNEA ARE RELATED TO THE YP4 FOLLICULAR EPITHELIUM YOLK PROTEIN SUBUNIT GENE OF PYRALID MOTHS - (Peer Reviewed Journal)
Cheon, H.M., Kim, H.J., Yun, C.Y., Lee, H.J., Lee, I.H., Shirk, P.D., Seo, S.J. Fat body expressed yolk protein genes in Hyphantria cunea are related to the YP4 follicular epithelium yolk protein subunit gene of pyralid moths. Insect Molecular Biology. 2003. v.12(4). p.383-392.
A PIGGYBAC-DERIVED VECTOR EFFICIENTLY PROMOTES GERM-LINE TRANSFORMATION IN THE SILKWORM BOMBYX MORI L. - (Peer Reviewed Journal)
A CDNA OF YP4, A FOLLICULAR EPITHELIUM YOLK PROTEIN SUBUNIT, IN THE MOTH, PLODIA INTERPUNCTELLA. - (Peer Reviewed Journal)
ALPHA-CRYSTALLIN PROTEIN COGNATES ARE CHAPERONES FOR FOLLICULAR EPITHELIUM YOLK PROTEIN IN EGGS OF THE MOTH, PLODIA INTERPUNCTELLA - (Peer Reviewed Journal)
Identification and analysis of the major yolk polypeptide from the caribbean fruit fly, Anastrepha suspensa (Loew) - (Peer Reviewed Journal)
Handler, A.M., Galette, A.S. 1988. Identification and analysis of the major yolk polypeptide from the caribbean fruit fly, Anastrepha suspensa (Loew). Archives of Insect Biochemistry and Physiology. 9:91-106.