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United States Department of Agriculture

Agricultural Research Service

Jeffrey S. Karns

Research Microbiologist


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Molecular detection of the index case of a subclinical salmonella Kentucky epidemic on a dairy farm - (Peer Reviewed Journal)
Haley, B.J., Allard, M., Brown, E., Hovingh, E., Karns, J.S., Van Kessel, J.S. 2014. Molecular detection of the index case of a subclinical salmonella Kentucky epidemic on a dairy farm. Epidemiology and Infection. DOI: 10.1017/S.0950268814001289.
Genome sequences of ten Salmonella enterica serovars isolated from a single dairy farm - (Peer Reviewed Journal)
Haley, B.J., Luo, Y., Wang, C., Pettengill, J., Allard, M., Brown, E., Karns, J.S., Van Kessel, J.S. 2014. Genome sequences of ten Salmonella enterica serovars isolated from a single dairy farm. Genome Announcements. 2(2):00082-14.
Phylogenetic Diversity of the Enteric Pathogen Salmonella enterica subsp. enterica Inferred from Genome-Wide Reference-Free SNP Characters - (Peer Reviewed Journal)
Timme, R., Pettengrll, J., Allard, M., Strain, E., Barrangou, R., Wehnes, C., Van Kessel, J.S., Karns, J.S., Musser, S., Brown, E. 2013. Phylogenetic Diversity of the Enteric Pathogen Salmonella enterica subsp. enterica Inferred from Genome-Wide Reference-Free SNP Characters. Genome Biology and Evolution. 5:2109-2123.
Determining RNA quality for NextGen sequencing: some exceptions to the gold standard rule of 23S to 16S rRNA ratio - (Peer Reviewed Journal)
Bhagwat, A.A., Ying, I., Karns, J.S., Smith, A.D. 2013. Determining RNA quality for NextGen sequencing: some exceptions to the gold standard rule of 23S to 16S rRNA ratio. Advances in Microbiology. 1:10.
Comparative genomics reveals the source and mechanisms of a Salmonella Kentucky epidemic on a dairy farm - (Abstract Only)
Haley, B.J., Allard, M., Brown, E., Hovingh, E., Karns, J.S., Van Kessel, J.S. 2013. Comparative genomics reveals the source and mechanisms of a Salmonella Kentucky epidemic on a dairy farm. BARC Poster Day.
Regional distribution of two dairy-associated Salmonella enterica serotypes - (Peer Reviewed Journal)
Van Kessel, J.S., Karns, J.S., Wolfgang, D.R., Hovingh, E. 2013. Regional distribution of two dairy-associated Salmonella enterica serotypes. Foodborne Pathogens and Disease. 10(5):448-452.
Genomic investigation of Salmonella enterica sequences associated with long-term colonization of the bovine gut - (Abstract Only)
Haley, B.J., Karns, J.S., Van Kessel, J.S. 2012. Genomic investigation of Salmonella enterica sequences associated with long-term colonization of the bovine gut. Meeting Abstract.
Antimicrobial resistance of Salmonella enterica isolates from bulk tank milk and milk filters in the United States - (Peer Reviewed Journal)
Van Kessel, J.S., Sonnier, J.L., Zhao, S., Karns, J.S. 2013. Antimicrobial resistance of Salmonella enterica isolates from bulk tank milk and milk filters in the United States. Journal of Food Protection. DOI: 10.4315/0362-28X.JFP-12-263.
Antimicrobial resistance of Salmonella enterica isolated from bulk tank milk and milk filters in the United States - (Abstract Only)
Van Kessel, J.S., Sonnier, J.L., Zhao, S., Karns, J.S. 2012. Antimicrobial resistance of Salmonella enterica isolated from bulk tank milk and milk filters in the United States. Meeting Abstract.
Dynamics of Salmonella serotype shifts in an endemically infected dairy herd - (Peer Reviewed Journal)
Van Kessel, J.S., Karns, J.S., Wolfgang, D., Hovingh, E., Schukken, Y. 2012. Dynamics of Salmonella serotype shifts in an endemically infected dairy herd. Foodborne Pathogens and Disease. 9:319-324.
Evaluation of Microbial Water Quality Indicators in a Forested and Agricultural Watershed Reprint Icon - (Peer Reviewed Journal)
Shelton, D.R., Karns, J.S., Sadeghi, A.M., Coppock, C.R., Pachepsky, Y.A. 2011.Relationship between eae and stx virulence genes and Escherichia coli in an agricultural watershed: Implications for irrigation water standards and leafy green commodities. Journal of Food Protection. 74(1):18-23.
Detection of E. coli O157:H7 by immunomagnetic separation coupled with fluorescence immunoassay - (Peer Reviewed Journal)
Zhu, P., Shelton, D.R., Li, S., Adams, D., Karns, J.S., Amstutz, P., Tang, C. 2011. Detection of E. coli O157:H7 by immunomagnetic separation coupled with fluorescence immunoassay. Biosensors and Bioelectronics. 30:337-341.
Effects of temperature on bacterial transport and destruction in bioretention media: Field and laboratory evaluations - (Peer Reviewed Journal)
Zhang, L., Seagren, E., Davis, A., Karns, J.S. 2012. Effects of temperature on bacterial transport and destruction in bioretention media: Field and laboratory evaluations. Water Environment Research. 84:485-496.
Quantitative risk assessment of Listeriosis due to consumption of raw milk - (Peer Reviewed Journal)
Latorre, A., Pradhan, A., Van Kessel, J.S., Karns, J.S., Boor, K., Rice, D., Mangione, K., Grohn, Y., Schukken, Y. 2011. Quantitative risk assessment of Listeriosis due to consumption of raw milk. Journal of Food Protection. 74(8): 1268-1281.
Increased in vitro adherence and on-farm persistence of predominant and persistent Listeria monocytogenes strains in the milking system Reprint Icon - (Peer Reviewed Journal)
Latorre, A., Van Kessel, J.S., Karns, J.S., Zurakowski, M., Pradhan, A., Sukhnanand, S., Schukken, Y. 2011. Increased in vitro adherence and on-farm persistence of predominant and persistent Listeria monocytogenes strains in the milking system. Applied and Environmental Microbiology. 77(11):3676-3684.
Gene expression during excystation of Cryptosporidium parvum oocysts - (Peer Reviewed Journal)
Jenkins, M.C., Obrien, C.N., Miska, K.B., Schwartz, R., Karns, J.S., Santin, M., Fayer, R. 2011. Gene expression during excystation of Cryptosporidium parvum oocysts. Parasitology Research. 109(2):509-513.
Long-term Sustainability of Escherichia coli Removal in Conventional Bioretention Media - (Peer Reviewed Journal)
Zhang, L., Seagren, E.A., Davies, A.P., Karns, J.S. 2011. Long-term Sustainability of Escherichia coli Removal in Conventional Bioretention Media. Journal of Environmental Engineering. 137(8):651-759.
Prevalence of Salmonella enterica, Listeria monocytogenes and E. coli Virulence Factors in Bulk Tank Milk and In-line Filters from US Dairies Reprint Icon - (Peer Reviewed Journal)
Van Kessel, J.S., Karns, J.S., Lombard, J.E. 2011. Prevalence of Salmonella enterica, Listeria monocytogenes and E. coli Virulence Factors in Bulk Tank Milk and In-line Filters from US Dairies. Foodborne Pathogens and Disease. 759-768.
Molecular Epidemiology of Mycobacterium avium subsp. paratuberculosis in a Longitudinal Study of Three Dairy Herds Reprint Icon - (Peer Reviewed Journal)
Pradhan, A., Mitchell, R., Kramer, A., Zurakowski, M., Fyock, T., Whitlock, R., Smith, J., Hovingh, E., Van Kessel, J.S., Karns, J.S., Schukken, Y. 2011. Molecular Epidemiology of Mycobacterium avium subsp. paratuberculosis in a Longitudinal Study of Three Dairy Herds. Journal of Clinical Microbiology. 49(3):893-901.
Comparison of generic E. coli vs. pathogenic E. coli virulence factors in an agricultural watershed: implications for irrigation water standards and leafy green commodities - (Peer Reviewed Journal)
Shelton, D.R., Karns, J.S., Coppock, C.R., Patel, J.R., Sharma, M., Pachepsky, Y.A. 2011. Comparison of generic E. coli vs. pathogenic E. coli virulence factors in an agricultural watershed: implications for irrigation water standards and leafy green commodities. Journal of Food Protection. 74:18-23.
Characterization of giardin protein expression during encystation of Giardia duodenalis - (Abstract Only)
Jenkins, M.C., Obrien, C.N., Macarisin, D., Karns, J.S., Santin, M., Fayer, R. 2010. Characterization of giardin protein expression during encystation of Giardia duodenalis. Meeting Abstract.
Correlation between Herrold’s egg yolk medium culture results and quantitative real-time PCR for Mycobacterium avium subspecies paratuberculosis in pooled fecal and environmental slurry samples Reprint Icon - (Peer Reviewed Journal)
Aly, S.S., Mangold, B., Anderson, R.J., Jiang, J., Shukken, Y., Hovingh, E., Wolfgang, D., Van Kessel, J.S., Karns, J.S., Lombard, J., Smith, J.M., Whitlock, R.H., Gardner, I.A. 2010. Correlation between Herrold’s egg yolk medium culture results and quantitative real-time PCR for Mycobacterium avium subspecies paratuberculosis in pooled fecal and environmental slurry samples. Journal of Veterinary Diagnostic Investigation. 22:677-683.
Tracking Zoonotic Pathogens in Dairy Production Chains - (Book / Chapter)
Van Kessel, J.S., Santin, M., Karns, J.S., Schukken, Y. 2010. Tracking Zoonotic Pathogens in Dairy Production Chains. In: Brul, S., Fratamico, P.M., and McMeekin, T.A., editors. Tracing Pathogens in the Food Chain. Philadelphia, PA: Woodhead Publishing. p. 503-526.
Identification of loci associated with tolerance to Johne's disease in Holstein cattle - (Peer Reviewed Journal)
Zanella, R., Settles, M., Mckay, S.D., Schnabel, R.D., Taylor, J.F., Fyock, T., Whitlock, R.H., Schukken, Y., Van Kessel, J.S., Karns, J.S., Hovingh, E., Smith, J., Neibergs, H.L. 2010. Identification of loci associated with tolerance to Johne's disease in Holstein cattle. Animal Genetics. 42(1):28-38.
Biofilm in milking equipment on a dairy farm as a potential source of bulk tank milk contamination with Listeria monocytogenes Reprint Icon - (Peer Reviewed Journal)
Latorre, A.A., Van Kessel, J.S., Karns, J.S., Zurakowski, M.J., Pradhan, A.K., Boor, K.J., Jayarao, B.M., Houser, B.A., Daugherty, C.S., Schukken, Y.H. 2010. Biofilm in milking equipment on a dairy farm as a potential source of bulk tank milk contamination with Listeria monocytogenes. Journal of Dairy Science. 93:2792-2802.
Factors associated with Salmonella presence in environmental samples and bulk-tank milk from U.S. dairies Reprint Icon - (Peer Reviewed Journal)
Ruzante, J., Lombard, J., Wagner, B., Fossler, C., Karns, J.S., Van Kessel, J.S., Gardner, I. 2010. Factors associated with Salmonella presence in environmental samples and bulk-tank milk from U.S. dairies. Zoonoses and Public Health. Available: http://dx.doi.org/10.1111/j.1863-2378.2010.01333.x
The capture and destruction of E. coli from simulated urban runoff using conventional bioretention media and iron oxide-coated sand - (Peer Reviewed Journal)
Zhang, L., Seagren, E.A., Davies, A.P., Karns, J.S. 2010. The capture and destruction of E. coli from simulated urban runoff using conventional bioretention media and iron oxide-coated sand. Water Environment Research. 82:701-713.
Salmonella, E. coli and Listeria in raw milk, a tale of 3 dairy farms in the northeast US - (Abstract Only)
Karns, J.S. 2009. Salmonella, E. coli and Listeria in raw milk, a tale of 3 dairy farms in the northeast US. Food Science and Nutrition Department, North Carolina Agricultural and Technical State University.
Motility revertants of opgGH mutants of Salmonella enterica serovar Typhimurium remain defective in mice virulence Reprint Icon - (Peer Reviewed Journal)
Kannan, P., Dharne, M., Smith, A.D., Karns, J.S., Bhagwat, A.A. 2009. Motility revertants of opgGH mutants of Salmonella enterica serovar Typhimurium remain defective in mice virulence. Current Microbiology. 59(6):641-645.
Usda/regional Dairy Quality Management Alliance (Rdqma) - 2009 Report - (Government Publication)
Schukken, Y., Van Kessel, J.S., Karns, J.S., Cray, P.J., Wolfgang, D., Hovingh, E., Whitlock, R., Smith, J., Pradhan, A. 2009. USDA/Regional Dairy Quality Management Alliance (RDQMA) - 2009 Report. Technical Report.
Pcr Analysis of Pathogenic E. Coli on Three Dairy Farms in the Northeastern United States - (Abstract Only)
Karns, J.S., Van Kessel, J.S. 2009. PCR Analysis of Pathogenic E. coli on Three Dairy Farms in the Northeastern United States. Joint Abstracts of the American Dairy Science and Society of Animal Science. J. Anim. Sci. Vol. 87, E-Suppl. 2/J. Dairy Sci. Vol. 92, E-Suppl. 1
Salmonella serotype shift during an endemic dairy infection - (Abstract Only)
Van Kessel, J.S., Karns, J.S. Salmonella serotype shift during an endemic dairy infection. Joint Abstracts of the American Dairy Science and Society of Animal Science. J. Anim. Sci. Vol. 87, E-Suppl. 2/J. Dairy Sci. Vol. 92, E-Suppl. 1 221
Genotypic diversity of Escherichia coli in a dairy farm Reprint Icon - (Peer Reviewed Journal)
Son, I., Van Kessel, J.S., Karns, J.S. 2009. Genotypic diversity of Escherichia coli in a dairy farm. Foodborne Pathogens and Disease. 6(7):837-847.
Molecular ecology of Listeria monocytogenes: Evidence for a reservoir in milking equipment on a dairy farm Reprint Icon - (Peer Reviewed Journal)
Latorre, A.A., Van Kessel, J.S., Karns, J.S., Zurakowski, M.J., Pradhan, A.K., Zadoks, R.N., Boor, K.J., Schukken, Y.H. 2009. Molecular ecology of Listeria monocytogenes: Evidence for a reservoir in milking equipment on a dairy farm. Applied and Environmental Microbiology. 75(5):1315-1323.
Dynamics of endemic infectious diseases of animal and human importance on three dairy herds in the northeastern United States Reprint Icon - (Peer Reviewed Journal)
Pradhan, A., Van Kessel, J.S., Karns, J.S., Wolfgang, D.R., Hovingh, E., Nelen, K.A., Smith, J.M., Whitlock, R.H., Fyock, T., Ladely, S.R., Cray, P.J., Schukken, Y.H. 2009. Dynamics of endemic infectious diseases of animal and human importance on three dairy herds in the northeastern United States. Journal of Dairy Science. 92:1811-1825.
Design of Integrating Waveguide Biosensor - (Book / Chapter)
Li, S., Amstutz, P., Tang, C., Hang, J., Sandaram, A., Zhu, P., Shelton, D.R., Karns, J.S. 2009. DESIGN OF INTEGRATING WAVEGUIDE BIOSENSOR. Springer Verlag. p .389-401.
Strain-dependent variations in attachment of E. coli to soil particles of different sizes - (Peer Reviewed Journal)
Pachepsky, Y.A., Karns, J.S., Yu, O., Shelton, D.R., Guber, A.K., Van Kessel, J.S. 2008. Strain-dependent variations in attachment of E. coli to soil particles of different sizes. International Agrophysics. 22:61-66.
Risk factors associated with the presence of viable Listeria monocytogenes in bulk tank milk from U.S. dairies Reprint Icon - (Peer Reviewed Journal)
Antognoli, M., Lombard, J.E., Wagner, B.A., Mcclusky, B.J., Van Kessel, J.S., Karns, J.S. 2009. Risk factors associated with the presence of viable Listeria monocytogenes in bulk tank milk from U.S. dairies. Zoonoses and Public Health. 56(2):77-83.
Environmental sampling to predict fecal prevalence of Salmonella in an intensively monitored dairy herd - (Peer Reviewed Journal)
Van Kessel, J.S., Karns, J.S., Wolfgang, D.R., Hovingh, E., Jayarao, B.M., Van Tassell, C.P., Schukken, Y.H. 2008. Environmental sampling to predict fecal prevalence of Salmonella in an intensively monitored dairy herd. Journal of Food Protection. 71(10):1967-1973.
A Multiple Protocol to Improve Diagnosis and Isolation of Shiga Toxin-Producing Escherichia coli (STEC) from Human Stool Specimens - (Peer Reviewed Journal)
Shelton, D.R., Karns, J.S., Park, C.H. 2008. A multiple protocol to improve diagnosis and isolation of shiga toxin-producing escherichia coli (stec) from human stool specimens. Diagnostic Microbiology and Infectious Disease. http://dx.DOI.org/10.1016/j.diagmicrobio.2008.05.001.
Loss of Bioactive Phosphorus and Enteric Bacteria in Runoff from Dairy Manure Applied to Sod Reprint Icon - (Peer Reviewed Journal)
Dao, T.H., Guber, A.K., Sadeghi, A.M., Karns, J.S., Van Kessel, J.S., Shelton, D.R., Pachepsky, Y.A., Mccarty, G.W. 2008. Loss of Bioactive Phosphorus and Enteric Bacteria in Runoff from Dairy Manure Applied to Sod. Soil Science. 173:511-521.
Usda/regional Dairy Quality Management Alliance (Rdqm) Project-2008 Report - (Government Publication)
Schyukken, Y., Van Kessel, J.S., Karns, J.S., Cray, P.J., Wolfgang, D., Whitlock, R., Smith, J., Nelen, K.A., Pradhan, A. 2008. USDA/Regional Dairy Quality Management Alliance (RDQM) Project-2008 Report. Technical Report.
Diversity of Escherichia Coli in a Dairy Farm - (Abstract Only)
Son, I., Van Kessel, J.S., Karns, J.S. 2008. Diversity of escherichia coli in a dairy farm. April 23, 2008. BARC Poster Day .
Whole Genome Association Study of Johne's Disease - (Abstract Only)
Settles, M., Zanella, R., Mckay, S., Schnabel, R., Taylor, J., Fyock, T., Whitlock, R., Schukken, Y., Hovingh, E., Van Kessel, J.S., Karns, J.S., Smith, J., Neibergs, H. 2008. Whole Genome Association Study of Johne's Disease. 3rd International Symposium on Animal Functional Genomics (ISAFG), April 7th-9th, 2008, Edinburg, UK.
Prevalence of E. coli Virulence Factors in Raw Bulk Tank Milk from U.S. Dairy Farms - (Abstract Only)
Karns, J.S., Van Kessel, J.S., Lombard, J.E. 2008. Prevalence of E. coli Virulence Factors in Raw Bulk Tank Milk from U.S. Dairy Farms. The American Society for Microbiology 108th General Meeting in Boston, Massachusetts, June 1- 5, 2008.
Prevalence of Salmonella and Listeria monocytogenes in Raw, Bulk Tank Milk From US Dairy Farms - (Abstract Only)
Van Kessel, J.S., Karns, J.S., Lombard, J.E. 2008. Prevalence of Salmonella and Listeria monocytogenes in Raw, Bulk Tank Milk From US Dairy Farms. 95th Annual Meeting of the International Association for Food Protection (IAFP) August 3-6, 2008, Columbus, Ohio. Paper No. P2-21.
Development of a Rapid and Sensitive Immunoassay for Detection and Subsequent Recovery of Bacillus anthracis Spores in Environmental Samples - (Peer Reviewed Journal)
Hang, J., Sundaram, A.K., Zhu, P., Shelton, D.R., Karns, J.S., Martin, P.A., Li, S., Amstutz, P., Tang, C.M. 2008. Development of a Rapid and Sensitive Immunoassay for Detection and Subsequent Recovery of Bacillus anthracis Spores in Environmental Samples. Journal of Microbial Methods. 73:242–246.
Comparison of Escherichia coli from Milk and Milk Filters with E. coli from Feces and Environmental Samples on a Dairy Farm - (Abstract Only)
Karns, J.S., Son, I., Van Kessel, J.S. 2008. Comparison of Escherichia coli from Milk and Milk Filters with E. coli from Feces and Environmental Samples on a Dairy Farm. The American Society for Microbiology 108th General Meeting, Boston, Massachusetts. June 1-5, 2008.
Diversity of Escherichia coli in a Dairy Farm - (Abstract Only)
Son, I., Van Kessel, J.S., Karns, J.S. 2008. Diversity of Escherichia coli in a Dairy Farm. The American Society for Microbiology 108th General Meeting in Boston, Massachusetts, June 1-5, 2008.
Heritability of Genetic Tolerance to Johne’s Disease - (Abstract Only)
Zanella, R., Settles, M., Fyock, T., Whitlock, R., Schukken, Y., Van Kessel, J.S., Karns, J.S., Hovingh, E., Smith, J., Van Tassell, C.P., Gaskins, C., Neibergs, H. 2008. Heritability of Genetic Tolerance to Johne’s Disease. Joint ADSA-ASAS Meeting, July 7th-11th, 2008, Indianapolis, Indiana. p.4.
Evaluation of Microbial Water Quality Indicators in a Forested/Agricultural Watershed - (Abstract Only)
Shelton, D.R., Karns, J.S., Sadeghi, A.M., Coppock, C.R., Pachepsky, Y.A. 2008. Evaluation of Microbial Water Quality Indicators in a Forested/Agricultural Watershed. Proceedings of the American Society of Agricultural and Biological Engineers International (ASABE). Online: http://asae.frymulti.com/abstract.asp?aid=24306&t=1.
Real-time PCR testing of pooled (1:5) fecal samples comparison to HEYM culture - (Proceedings)
Whitlock, R.H., Mangold, B.L., Mcadams, S., Fyock, T., Sweeney, R., Schukken, Y., Smith, J., Van Kessel, J.S., Hovingh, E., Karns, J.S., Wolfgang, D., Johnson, T. 2007. Real-time PCR testing of pooled (1:5) fecal samples comparison to HEYM culture. International Colloquium on Paratuberculosis, Tsukuba, Japan. No.75b. http://www.paratuberculosis.org/pubs/proc9/abst75b_o1.htm.
Longitudinal study of a clonal, sub-clinical outbreak of Salmonella enterica subsp. enterica Serovar Cerro in a US dairy herd - (Peer Reviewed Journal)
Van Kessel, J.S., Karns, J.S., Wolfgang, D.R., Hovingh, E., Schukken, Y. 2007. Longitudinal study of a clonal, sub-clinical outbreak of Salmonella enterica subsp. enterica Serovar Cerro in a US dairy herd. Foodborne Pathogens and Disease. 4(4):449-461.
Strain-dependent variations in attachment of E. coli to soil particles of different sizes - (Abstract Only)
Yu, O., Pachepsky, Y.A., Karns, J.S., Shelton, D.R., Guber, A.K. 2007. Strain-dependent variations in attachment of E. coli to soil particles of different sizes. ASA-CSSA-SSSA International Annual Meeting, November 4-8, 2007, New Orleans, LA. Abstract 292-24. 2007 CDROM.
Incidence of Escherichia coli O157:H7 and E. coli Virulence Factors in US Bulk Tank Milk as Deteremined by Polymerase Chain Reaction - (Peer Reviewed Journal)
Karns, J.S., Van Kessel, J.S., McClusky, B.J., Perdue, M.L. 2007. Incidence of Escherichia coli O157:H7 and E. coli Virulence Factors in U.S. Bulk Tank Milk as Deteremined by Polymerase Chain Reaction. Journal of Dairy Science. 90:3212-3219.
The relationship between Salmonella detection from milk filters and bulk milk and the fecal shedding prevalence of Salmonella in a dairy herd - (Abstract Only)
Van Kessel, J.S., Karns, J.S., Wolfgang, D.R., Hovingh, E., Schukken, Y. 2007. The relationship between Salmonella detection from milk filters and bulk milk and the fecal shedding prevalence of Salmonella in a dairy herd. Joint Meeting of the ADSA, AMSA, ASAS and PSA, July 7-12, 2007, San Antonio, Texas.
A Mathematical Model of the Dynamics of Salmonella Cerro Infection in a U.S. Dairy Herd - (Peer Reviewed Journal)
Chapagain, P.P., Van Kessel, J.S., Karns, J.S., Wolfgang, D.R., Hovingh, E., Nelen, K., Schukken, Y.H., Grohn, Y.T. 2007. A mathematical model of the dynamics of Salmonella Cerro infection in a U.S. dairy herd. Epidemiology and Infection. Available: http://Journals.Cambridge.org.
A Multiple Protocol to Improved Diagnosis and Isolation of Shiga toxin-Producing Escherichia coli (STEC) from Human Stool Specimens - (Abstract Only)
Shelton, D.R., Park, C., Karns, J.S. 2007. A multiple protocol to improved diagnosis and isolation of shiga toxin-producing escherichia coli (stec) from human stool specimens. 107th Annual Meeting of the American Society for Microbiology, May 21-25, 2007, Toronto, Canada.
Survival of Escherichia Coli in Cow Pats in Pasture and in Laboratory Conditions - (Peer Reviewed Journal)
Van Kessel, J.S., Pachepsky, Y.A., Shelton, D.R., Karns, J.S. 2007. Survival of Escherichia coli in cow pats in pasture and in laboratory conditions. Journal of Applied Microbiology. 103(4):1122-1127.
Comparison of Release and Transport of Manure-Borne E. Coli and Enterococci under Grass Buffer Conditions - (Peer Reviewed Journal)
Guber, A.K., Karns, J.S., Pachepsky, Y.A., Sadeghi, A.M., Van Kessel, J.S., Dao, T.H. 2007. Comparison of release and transport of manure-borne E. coli and Enterococci under grass buffer conditions. Letters in Applied Microbiology. 44(2):161-167.
Impact of Microbial Diversity on Rapid Detection of Enterohemorrhagic Escherichia Coli in Surface Waters - (Peer Reviewed Journal)
Shelton, D.R., Karns, J.S., Higgins, J.A., Van Kessel, J.S., Perdue, M.L., Belt, K.T., Russel-Anelli, J., Debroy, C. 2006. Impact of microbial diversity on rapid detection of enterohemorrhagic Escherichia coli in surface waters. Federation of European Microbiological Societies Microbiology Letters. 261:95-101.
Differences in Release and Transport of Manure-Borne E. Coli and Enterococci in Grass Buffer Conditions - (Abstract Only)
Guber, A.A., Karns, J.S., Pachepsky, Y.A., Sadeghi, A.M., Van Kessel, J.S., Dao, T.H. 2006. Differences in release and transport of manure-borne E. coli and Enterococci in grass buffer conditions. ASA-CSSA-SSSA International Meeting, November 12-16, 2006, Indianapolis, Indiana. CD-ROM.
A Long Term, Sub-Clinical, Outbreak of Salmonella Enterica Subsp. Enterica Cerro in a Pennsylvania Dairy Herd - (Abstract Only)
Van Kessel, J.S., Karns, J.S., Wolfgang, D.R., Hovingh, E., Schukken, Y. 2006. A long term, sub-clinical, outbreak of Salmonella enterica subsp. enterica Cerro in a Pennsylvania dairy herd. Joint Meeting of the ADSA, AMSA, ASAS and PSA, Minneapolis, MN, July 9-13, 2006.
Impact of Microbial Diversity on Detection of Water-Borne Enterohemorrhagic Escherichia Coli - (Abstract Only)
Shelton, D.R., Karns, J.S., Higgins, J.A., Van Kessel, J.S., Belt, K.T., Debroy, C. 2006. Impact of microbial diversity on detection of water-borne enterohemorrhagic Escherichia coli [abstract]. 2nd FEMS Congress of European Microbiologists, July 4-8, 2006, Madrid, Spain.
Detection of Pathogenic Agents Using the Integrating Waveguide Biosensor - (Abstract Only)
Shelton, D.R., Zhu, P., Hang, J., Li, S., Karns, J.S., Amstutz, P., Tang, C. 2006. Detection of pathogenic agents using the integrating waveguide biosensor [abstract]. The Ninth World Congress on Biosensors, May 10-12, 2006, Toronto, Ontario, Canada.
Detection of Pathogenic Agents Using the Integrating Waveguide Biosensor - (Abstract Only)
Tang, C., Shelton, D.R., Zhu, P., Hang, J., Li, S., Karns, J.S., Amstutz, P. 2006. Detection of pathogenic agents using the Integrating Waveguide Biosensor. ASM Biodefense Research Meeting, February 15-18, 2006, Baltimore, MD. p.39.
Mathematical Modeling of the Dynamics of Salmonella Cerro Infection in a Us Dairy Herd - (Abstract Only)
Chapagain, P.P., Van Kessel, J.S., Karns, J.S., Wolfgang, D., Schukken, Y.H., Grohn, Y.T. 2006. Mathematical modeling of the dynamics of Salmonella Cerro infection in a U.S. dairy herd. American Physical Society. p. 1.
Subtyping Listeria Monocytogenes from Bulk Tank Milk Using Automated Repetitive Element-Based Pcr - (Peer Reviewed Journal)
Van Kessel, J.S., Karns, J.S., Gorski, L.A., Perdue, M.L. 2006. Subtyping Listeria monocytogenes from bulk tank milk using automated repetitive element-based PCR. Journal of Food Protection. 6:2707-2712.
Prevalanece of Salmonella Enterica in Bulk Tank Milk from U.S. Dairies As Determined by Pcr - (Peer Reviewed Journal)
Karns, J.S., Van Kessel, J.S., Mccluskey, B.J., Perdue, M.L. 2005. Prevalanece of Salmonella Enterica in bulk tank milk from U.S. Dairies as determined by pcr. Journal of Dairy Science. 88:3475-3479.
Rapid Germination and Growth of Bacillus Anthracis - (Abstract Only)
Hang, J., Sundaram, A., Shelton, D.R., Karns, J.S., Zhu, P., Amstutz, P., Tang, C. 2005. Rapid Germination and Growth of Bacillus Anthracis. ASM Biodefense Research Meeting, March 20-23, 2005, Baltimore, MD. p.60.
Sensitive and Rapid Biosensor and Assays for Detection of Bacillus Anthracis Spores - (Abstract Only)
Sundaram, A., Hang, J., Shelton, D.R., Karns, J.S., Zhu, P., Li, S., Amstutz, P., Bull, R., Tang, C. 2005. Sensitive and Rapid Biosensor and Assays for Detection of Bacillus Anthracis Spores. ASM Biodefense Research Meeting, March 20-23, 2005, Baltimore, MD. p.60.
A Biosensor Platform for Genetic Identification and Quantitation of Bacillus Anthracis - (Abstract Only)
Zhu, P., Shelton, D.R., Higgins, J.A., Karns, J.S., Bull, R.L., Martin, P.A., Amstutz, P., Tang, C. 2005. A Biosensor Platform for Genetic Identification and Quantitation of Bacillus Anthracis. ASM Biodefense Research Meeting, March 20-23, 2005, Baltimore, MD.
Subtyping Listeria Monocytogenes from Bulk Tank Milk Using Automated Repetitive Element-Based Pcr - (Proceedings)
Van Kessel, J.S., Karns, J.S., Gorski, L.A., Perdue, M.L. 2005. Subtyping Listeria monocytogenes from bulk tank milk using automated repetitive element-based PCR. In: International Association for Food Protection Proceedings, August 14-17, 2005, Baltimore, MD. p. 14.
Detection of Water Borne E. Coli Using the Integrated Waveguide Biosensor - (Peer Reviewed Journal)
Shu, P., Shelton, D.R., Karns, J.S., Sundaram, A., Li, S., Amstutz, P., Tang, C. 2005. Detection of water borne E. coli using the integrated waveguide biosensor. Biosensors and Bioelectronics. 21:678-683.
Detecting Pathogens in Cattle and Meat - (Book / Chapter)
Fratamico, P.M., Gehring, A.G., Karns, J.S., Van Kessel, J.S. 2005. Detecting pathogens in cattle and meat. Book Chapter. Improving the Safety of Fresh Meat, Woodhead Publishing Ltd., Cambridge, UK pg:25-55
Tir and Stx Positive E. Coli in Stream Waters in a Metropolitan Area - (Peer Reviewed Journal)
Higgins, J.A., Karns, J.S., Shelton, D.R., Belt, K.T., Anelli, J. 2005. Tir- and stx- positive E. coli in stream waters in a metropolitan area. Applied and Environmental Microbiology. 71:2511-2519.
Analysis of Bacillus Anthracis Spores in Milk Using Mass Spectrometry - (Peer Reviewed Journal)
Whiteaker, J., Karns, J.S., Fenselau, C., Perdue, M.L. 2004. Analysis of Bacillus anthracis spores in milk using mass spectrometry. Foodborne Pathogens and Disease. l(3):185-194.
Prevalence of Salmonellae, Listeria Monocytogenes and Fecal Coliforms in Bulk Tank Milk on U.S. Dairies - (Peer Reviewed Journal)
Van Kessel, J.S., Karns, J.S., Gorski, L.A., McCluskey, B.J., Perdue, M.L. 2004. Prevalence of Salmonellae, Listeria monocytogenes and fecal coliforms in bulk tank milk on U.S. dairies. Journal of Dairy Science. p. 2822-2830.
Estimation of Viable Escherichia Coli 0157 in Surface Waters Using Enrichment in Conjunction with Immunological Detection - (Peer Reviewed Journal)
Shelton, D.R., Higgins, J.A., Van Kessel, J.S., Pachepsky, Y.A., Belt, K., Karns, J.S. 2004. Estimation of viable Escherichia coli 0157 in surface waters using enrichment in conjunction with immunological detection. Journal of Microbiological Methods. 58:223-231.
Prevalence of Salmonella Enterica in Bulk Tank Milk from Us Dairies As Determined by Pcr - (Abstract Only)
Karns, J.S., Van Kessel, J.S., McCluskey, B.J., Perdue, M.L. 2005. Prevalence of Salmonella enterica in bulk tank milk from US Dairies as determined by PCR. International Association for Food Protection Proceedings, August 14-17, 2005, Baltimore, MD. p.1
A Combined Immunological-Pcr Biosensor for Detection of Water Borne E. Col 0157:h7 - (Abstract Only)
Zhu, P., Shelton, D.R., Karns, J.S., Sundaram, A., Li, S., Amstutz, P., Tang, C. 2004. A combined immunological-PCR biosensor for detection of water borne E. coli O157:H7. American Society of Microbiology Meeting, Bio-Defense Research. p. 24.
Surveying the Baltimore Ecosystem Study Stream Sites for the Presence of Enteric Pathogens - (Abstract Only)
Higgins, J.A., Shelton, D.R., Karns, J.S., Russell-Anelli, J., Belt, K. 2003. Surveying the Baltimore Ecosystem study stream sites for the presence of enteric pathogens [Abstract]. Baltimore Ecosystem Study Annual Meeting. Brochure p.1.
Detection and Characterization of Pathogenic E. Coli in Surface Waters: Implications for Preharvest Produce - (Abstract Only)
Shelton, D.R., Pachepsky, Y.A., Belt, K.T., Debroy, C., Karns, J.S. 2003. Detection and characterization of pathogenic E. coli in surface waters: Implications for preharvest produce. United States-Japan Cooperative Program in Natural Resources. p. 12.
Evaluation of Parameters Affecting Detection of Escherichia Coli 0157 in Enriched Water Samples Using Immunomagnetic Electrochemiluminescence - (Peer Reviewed Journal)
Shelton, D.R., Van Kessel, J.S., Wachtel, M.R., Belt, K.T., Karns, J.S. 2003. Evaluation of parameters affecting detection of Escherichia Coli 0157 in enriched water samples using immunomagnetic electrochemiluminescence. Journal of Microbiological Methods. p. 717-725.
Detection and Fate of Bacillus Anthracis (Sterne) Vegetative Cells and Spores Added to Bulk Tank Milk - (Peer Reviewed Journal)
Perdue, M.L., Karns, J.S., Higgins, J.A., Van Kessel, J.S. 2003. Detection and fate of Bacillus anthracis (Sterne) vegetative cells and spores added to bulk tank milk. Journal of Food Protection. 66:2349-2354.
Use of Real-Time Polymerase Chain Reactions(pcr) for the Detection of Pathogenic Microbes in Bulk Tank Milk - (Abstract Only)
Karns, J.S., Van Kessel, J.S., Perdue, M.L. 2003. Use of Real-Time Polymerase Chain Reactions(PCR) for the detection of pathogenic microbes in bulk tank milk. Joint Abstracts of the American Dairy Science and Society of Animal Science. p.369
Survey of Bulk Tank Milk in the United States for Food-Borne Bacterial Pathogens - (Abstract Only)
Van Kessel, J.S., Karns, J.S., McCluskey, B.J., Perdue, M.L. 2003. Survey of bulk tank milk in the United States for food-borne bacterial pathogens. [Abstract]. Joint Abstracts of the American Dairy Science and Society of Animal Science. p.369.
Using Portable Real-Time Polymerase Chain Reaction (Rt-Pcr) Assays to Detect Salmonella Spp. in Raw Milk - (Abstract Only)
VAN KESSEL, J.S., KARNS, J.S., PERDUE, M.L. USING PORTABLE REAL-TIME POLYMERASE CHAIN REACTION (RT-PCR) ASSAYS TO DETECT SALMONELLA SPP. IN RAW MILK. AMERICAN SOCIETY FOR MICROBIOLOGY. 2003. P-068.
Using Portable Real-Time Polymerase Chain Reaction (Rt-Pcr) Assays to Detect Salmonella in Raw Milk - (Peer Reviewed Journal)
VAN KESSEL, J.S., KARNS, J.S., PERDUE, M.L. USING PORTABLE REAL-TIME POLYMERASE CHAIN REACTION (RT-PCR) ASSAYS TO DETECT SALMONELLA IN RAW MILK. JOURNAL OF FOOD PROTECTION. 2003. pp.1762-1767.
Specific Detection of Pathogens in Environmental Samples: Agricultural Research Meets Bioterrorism - (Abstract Only)
Karns, J.S. 2003. Specific detection of pathogens in environmental samples: Agricultural research meets bioterrorism [abstract]. 53rd Annual Meeting Canadian Society of Microbiologists, May 25-28, 2003, Ville de Laval, Montreal, Canada. p. 1.
Real-Time Pcr Assays for the Quantitation of Enteropathogenic E.COLI in Water - (Abstract Only) - (01-May-03)
Estimating Escherichia Coli O157 Populations in Water Samples Using Immunomagnetic Electrochemiluminescence - (Abstract Only)
Shelton, D.R., Van Kessel, J.S., Karns, J.S. 2003. Estimating Escherichia coli O157 populations in water samples using immunomagnetic ectrochemiluminescence. American Society for Microbiology Annual Meeting. p.96
Molecular Tools to Evaluate the Epidemiology and Control of Waterborne Zoonotic Infections - (Abstract Only)
Higgins, J.A., Shelton, D.R., Karns, J.S., Belt, K. 2003. Molecular tools to evaluate the epidemiology and control of waterborne zoonotic infections. American Association of Veterinary Parasitologists Proceedings, July 22, 2003.Denver, Colorado. p. 61.
Prevalence and Molecular Characterization of Enteropathogenic E. Coli in Streams Along An Urban-Rural Gradient in the Baltimore, Maryland Metropolitan Area - (Abstract Only)
Higgins, J.A., Karns, J.S., Shelton, D.R., Belt, K. 2003. Prevalence and molecular characterization of enteropathogenic E. coli in streams along an urban-rural gradient in the Baltimore, Maryland Metropolitan Area [abstract]. American Society for Microbiology Annual Meeting. p. 125.
Release Rates of Manure-Borne Coliform Bacteria from Data on Leaching Through Stony Soil - (Peer Reviewed Journal)
Shelton, D.R., Pachepsky, Y.A., Sadeghi, A.M., Stout, W.L., Karns, J.S., Gbarek, W.J. 2003. Release rates of manure-borne coliform bacteria from data on leaching through stony soil. Vadose Zone Journal. 2:34-39.
A Handheld Real Time Thermal Cycler for Bacterial Pathogen Detection - (Peer Reviewed Journal)
Higgins, J.A., Nasarabadi, S., Karns, J.S., Shelton, D.R., Cooper, M., Gbakima, A., Koopman, R.P. 2003. A handheld real time thermal cycler for bacterial pathogen detection. Biosensors and Bioelectronics. p. 115-1123.
Influence of Rhamnolipids and Triton X-100 on the Desorption of Pesticides from Soils - (Peer Reviewed Journal) - (12-Aug-02)
Animal Manure: Bacterial Pathogens and Disinfection Technologies - (Peer Reviewed Journal) - (01-Aug-02)
Fluorescent Real-Time Molecular (Pcr) Detection of Zoonotic Bacteria and Viruses in Bulk Milk. - (Abstract Only)
Perdue, M.L., Karns, J.S., Van Kessel, J.S., Higgins, J.A. 2002. Fluorescent Real-Time Molecular (PCR) Detection of zoonotic bacteria and viruses in bulk milk. p.3. . International Meeting on Molecular Epidemiology and Evolutionary Genetics in Infectious Disease.
Mobile Laboratory for Bacillus Anthracis Detection - (Proceedings)
Black, S., Higgins, J.A., Karns, J.S., Perdue, M.L., Breeze, R., House, D., Cooper, M., Tucker, L., Miller, D. 2002. Mobile laboratory for Bacillus anthracis detection. National Cancer Institute, United States Army Medical Institute of Infectious Disease, United States Department of Agriculture Spring Research Fair.
A Field Investigation of Bacillus Anthracis Contamination of U.S. Department of Agriculture and Other Washington, D.C., Buildings During the Anthrax Attack of October 2001 - (Peer Reviewed Journal)
Higgins, J.A., Cooper, M., Tucker, L., Black, S., Miller, D., Karns, J.S., Manthey, E., Breeze, R., Perdue, M.L. 2003. A field investigation of Bacillus anthracis contamination of U.S. Department of Agriculture and other Washington, D.C., buildings during the anthrax attack of October 2001. Applied and Environmental Microbiology. 69:593-599.
Rapid Extracton of DNA from Escherichia Coli and Cryptosporidium Parvum for Use in Pcr - (Peer Reviewed Journal)
Higgins, J.A., Jenkins, M.C., Shelton, D.R., Fayer, R., Karns, J.S. 2001. Rapid extracton of dna from escherichia coli and cryptosporidium parvum for use in pcr. Applied and Environmental Microbiology. 67:5321-5324.
Survival of E. Coli 0157:h7 from Manure and Irrigation Water in Soil and in the Presence of Cover Crops - (Peer Reviewed Journal) - (01-Jul-01)
The Influence of Rhamnolipids and Triton X-100 on the Biodegradation of Three Pesticides in Aqueous Phase and Soil Slurries - (Peer Reviewed Journal) - (27-Apr-01)
Quantitative Detection of Escherichia Coli O157 in Surface Waters Using Immunomagnetic-Electrochemiluminescence (Im-Ecl) - (Peer Reviewed Journal)
SHELTON, D.R., KARNS, J.S. QUANTITATIVE DETECTION OF ESCHERICHIA COLI O157 IN SURFACE WATERS USING IMMUNOMAGNETIC-ELECTROCHEMILUMINESCENCE (IM-ECL). APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2001.
Effect of Rhamnolipids Produced by P. Aeruginosa Ug2 on the Solubilization of Pesticides. - (Peer Reviewed Journal) - (01-Sep-00)
Survival of E. Coli 0157:h7 in Soil - (Abstract Only) - (01-May-00)
The Influence of Rhamnolipids and Triton X-100 on the Biodegradation of Three Pesticides in Aqueous Phase and Soil Slurries. - (Peer Reviewed Journal) - (28-Apr-00)
Effect of Nutritional and Environmental Conditions on the Production and Composition of Rhamnolipids by P. Aeruginosa Ug2. - (Peer Reviewed Journal) - (18-Mar-00)
Leaching of E. Coli 0157:h7 in Diverse Soils under Various Agricultural Management Practices - (Peer Reviewed Journal) - (06-Dec-99)
Hplc Method for the Characterization of Rhamnolipid Mixtures Produced by P.AERUGINOSA Ug2 on Corn Oil - (Peer Reviewed Journal) - (01-Nov-99)
Last Modified: 3/29/2013
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