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United States Department of Agriculture

Agricultural Research Service

JAMES L. BONO (JIM)

Research Microbiologist


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Evaluation of real time PCR assays for the detection and enumeration of enterohemorrhagic Escherichia coli directly from cattle feces - (Peer Reviewed Journal) - (13-Jul-14)
Exploiting the explosion of information associated with whole genome sequencing to tackle Shiga toxin-producing Escherichia coli (STEC) in global food production systems - (Other)
Franz, E., Delaquis, P., Stefano, M., Beutin, L., Gobius, K., Rasko, D., Bono, J.L., French, N., Osek, J., Lindstedt, B., Muniesa, M., Manning, S., LeJeune, J., Callaway, T.R., Beatson, S., Eppinger, M., Dallman, T., Forbes, K., Aarts, H., Pearl, D., Strachan, N.J. 2014. Exploiting the explosion of information associated with whole genome sequencing to tackle Shiga toxin-producing Escherichia coli (STEC) in global food production systems. International Journal of Food Microbiology. 187:57-72.
Fecal microbiome in the bovine animal - (Proceedings)
Wells, J., Kim, M.S., Kuehn, L.A., Bono, J.L. 2014. Fecal microbiome in the bovine animal. In: Proceedings of the Americian Meat Science Association. Reciprocal Meat Conference, 6/15-6/18/2014, Madison Wisconsin. http://www.meatscience.org/.
Effect of proximity to a cattle feedlot on Escherichia coli O157:H7 contamination of leafy greens and evaluation of the potential for bioaerosol and pest fly transmission - (Abstract Only)
Berry, E.D., Wells, J., Durso, L.M., Bono, J.L., Friesen, K.M., Woodbury, B.L., Kalchayanand, N., Norman, K.N., Suslow, T.V., Lopez-Velasco, G., Millner, P.D. 2014. Effect of proximity to a cattle feedlot on Escherichia coli O157:H7 contamination of leafy greens and evaluation of the potential for bioaerosol and pest fly transmission. [Abstract]. Western Food Safety Summit, May 8-9, 2014, Salinas, CA. poster session.
Complete genome sequence and comparison of two Shiga toxin-producing Escherichia coli O104 isolates - (Abstract Only)
Yan, X., Fratamico, P.M., Bono, J.L., Baranzoni, G., Tebbs, R., O'Connell, C. 2014. Complete genome sequence and comparison of two Shiga toxin-producing Escherichia coli O104 isolates. Meeting Abstract. MA.
Complete closed genome sequences of three Bibersteinia trehalosi nasopharyngeal isolates from cattle with shipping fever - (Peer Reviewed Journal)
Harhay, G.P., McVey, D.S., Koren, S., Phillippy, A.M., Bono, J.L., Harhay, D.M., Clawson, M.L., Heaton, M.P., Chitko-McKown, C.G., Korlach, J., Smith, T.P. 2014. Complete closed genome sequences of three Bibersteinia trehalosi nasopharyngeal isolates from cattle with shipping fever. Genome Announcements. 2(1):e00084-14.
Complete closed genome sequences of four Mannheimia varigena isolates from cattle with shipping fever - (Peer Reviewed Journal)
Harhay, G.P., Murray, R.W., Lubbers, B., Griffin, D., Koren, S., Phillippy, A.M., Harhay, D.M., Bono, J.L., Clawson, M.L., Heaton, M.P., Chitko-McKown, C.G., Smith, T.P. 2014. Complete closed genome sequences of four Mannheimia varigena isolates from cattle with shipping fever. Genome Announcements. 2(1):e00088-14.
The first closed genome sequence of Campylobacter fetus subsp. venerealis biovar intermedius - (Peer Reviewed Journal)
Van Der Graff, L., Miller, W.G., Yee, E., Bono, J.L., Rijnsburger, M., Campero, C., Wagenaar, J.A., Duim, B. 2014. The first closed genome sequence of Campylobacter fetus subsp. venerealis biovar intermedius. Genome Announcements. 2(1):e01246-13.
Escherichia coli O157:H7, diet, and fecal microbiome in beef cattle - (Proceedings)
Wells, J., Kim, M.S., Bono, J.L., Kuehn, L.A., Benson, A.K. 2014. Escherichia coli O157:H7, diet, and fecal microbiome in beef cattle. In: Proceedings of American Society of Animal Science, Meat Science and Muscle Biology Symposium. Journal of Animal Science. 92:1345-1355.
Investigation of bacterial diversity in the feces of cattle fed different diets Reprint Icon - (Peer Reviewed Journal)
Kim, M.S., Kim, J., Kuehn, L.A., Bono, J.L., Berry, E.D., Kalchayanand, N., Freetly, H.C., Benson, A.K., Wells, J. 2014. Investigation of bacterial diversity in the feces of cattle fed different diets. Journal of Animal Science. 92:683-694.
Characterization of escherichia coli O157:H7 strains from contaminated raw beef trim during “high event periods” Reprint Icon - (Peer Reviewed Journal)
Arthur, T.M., Bono, J.L., Kalchayanand, N. 2014. Characterization of escherichia coli O157:H7 strains from contaminated raw beef trim during “high event periods”. Applied and Environmental Microbiology. 80(2):506-514.
Lineage and genogroup-defining single nucleotide polymorphisms of Escherichia coli 0157:H7 Reprint Icon - (Peer Reviewed Journal)
Jung, W.K., Bono, J.L., Clawson, M.L., Leopold, S.R., Shringi, S., Besser, T.E. 2013. Lineage and genogroup-defining single nucleotide polymorphisms of Escherichia coli 0157:H7. Applied and Environmental Microbiology. 79(22):7036-7041.
Reducing assembly complexity of microbial genomes with single-molecule sequencing - (Peer Reviewed Journal)
Koren, S., Harhay, G.P., Smith, T.P.L., Bono, J.L., Harhay, D.M., McVey, D.S., Radune, D., Bergman, N.H., Phillippy, A.M. 2013. Reducing assembly complexity of microbial genomes with single-molecule sequencing. Genome Biology. 14:R101. DOI: 10.1186/GB-2013-14-9-R101.
Proposed model for the high rate of rearrangement and rapid migration observed in some IncA/C plasmid lineages - (Peer Reviewed Journal)
Meinersmann, R.J., Lindsey, R., Bono, J.L., Smith, T.P., Oakley, B. 2013. Proposed model for the high rate of rearrangement and rapid migration observed in some IncA/C plasmid lineages. Applied and Environmental Microbiology. 79(16):4806-4814.
Diet, fecal microbiome and Escherichia coli O157:H7 shedding in beef Cattle - (Abstract Only)
Wells, J., Kim, M.S., Bono, J.L., Kuehn, L.A., Benson, A.K. 2013. Diet, fecal microbiome and Escherichia coli O157:H7 shedding in beef cattle.[abstract] Journal of Animal Science Supplement. 96(E-Supplement 2):631. Abstract 577.
Investigation of microbial diversity in the feces of cattle fed different diets - (Abstract Only)
Kim, M.S., Kim, J., Kuehn, L.A., Bono, J.L., Berry, E.D., Kalchayanand, N., Freetly, H.C., Benson, A.K., Wells, J. 2013. Investigation of microbial diversity in the feces of cattle fed different diets.[abstract] Journal of Animal Science Supplement. 91 (E-Supplement 2): 235.
Characterization of Escherichia coli 0157:H7 strains isolated from supershedding cattle Reprint Icon - (Peer Reviewed Journal)
Arthur, T.M., Ahmed, R., Chase-Topping, M., Kalchayanand, N., Schmidt, J.W., Bono, J.L. 2013. Characterization of Escherichia coli 0157:H7 strains isolated from supershedding cattle. Applied and Environmental Microbiology. 79(14):4294-4303.
Effect of proximity to a cattle feedlot on the occurrence of Escherichia coli 0157:H7-positive pest flies in a leafy green crop - (Abstract Only)
Berry, E.D., Wells, J., Durso, L.M., Bono, J.L., Friesen, K.M., Woodbury, B.L., Suslow, T.V., Lopez-Velasco, G., Millner, P.D. 2013. Effect of proximity to a cattle feedlot on the occurrence of Escherichia coli 0157:H7-positive pest flies in a leafy green crop. International Association for Food Protection Proceedings. [abstract]P3-132 Page No 65.
Characterization of E. coli O157:H7 strains isolated from super-shedding cattle - (Abstract Only)
Arthur, T.M., Ahmed, R., Chase-Topping, M., Kalchayanand, N., Schmidt, J.W., Bono, J.L. 2013. Characterization of E. coli O157:H7 strains isolated from super-shedding cattle. American Society for Microbiology General Meeting. Poster No. 1707, p 171.
Phylogenetic classification of Escherichia coli O26 strains from human, animals, and environmental origins using nucleotide polymorphisms - (Abstract Only)
Norman, K.N., Clawson, M.L., Strockbine, N.A., Mandrell, R.E., Johnson, R., Ziebell, K., Zhao, S., Fratamico, P.M., Stones, R., Allard, M., Bono, J.L. 2013. Phylogenetic classification of Escherichia coli O26 strains from human, animals, and environmental origins using nucleotide polymorphisms. American Society for Microbiology General Meeting. Poster No. 484 p. 40.
Salmonella virulence, genomics and interactions with the immune system - (Proceedings)
Harhay, D.M., Smith, T.P., Harhay, G.P., Bono, J.L., Bosilevac, J.M. 2013. Salmonella virulence, genomics and interactions with the immune system. Meeting Abstract. The Beef Industry Safety Summit, March 13-15, 2013, Dallas Texas, FLASHDRIVE.
Disinfectant and antibiotic susceptibility profiles of Escherichia coli O157:H7 strains from cattle carcasses, feces, and hides and ground beef from the United States Reprint Icon - (Peer Reviewed Journal)
Beier, R.C., Poole, T.L., Brichta-Harhay, D.M., Anderson, R.C., Bischoff, K.M., Hernandez, C.A., Bono, J.L., Arthur, T.M., Nagaraja, T.G., Crippen, T.L., Sheffield, C.L., Nisbet, D.J. 2013. Disinfectant and antibiotic susceptibility profiles of Escherichia coli O157:H7 strains from cattle carcasses, feces, and hides and ground beef from the United States. Journal of Food Protection. 76:6-17.
Dual-serotype biofilm formation by Shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains - (Peer Reviewed Journal)
SWang, R., Kalchayanand, N., Schmidt, J.W., Bosilevac, J.M., Bono, J.L. 2012. Dual-serotype biofilm formation by Shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains. Applied and Environmental Microbiology. 78(17):6341-6344.
Comparison of bacterial communities in faeces of beef cattle fed diets containing corn and wet distillers grain with solubles Reprint Icon - (Peer Reviewed Journal)
Durso, L.M., Wells, J., Harhay, G.P., Rice, W.C., Kuehn, L.A., Bono, J.L., Shackelford, S.D., Wheeler, T.L., Smith, T.P. 2012. Comparison of bacterial communities in faeces of beef cattle fed diets containing corn and wet distillers grain with solubles. Letters in Applied Microbiology. 55(2):109-14. DOI: 10.1111/J.1472-765X.2012.03265.X.
Association of nucleotide polymorphisms within the O-antigen gene cluster of Escherichia coli O26, O45, O103, O111, O121, and O145 with serogroups and genetic subtypes - (Peer Reviewed Journal)
Norman, K.N., Strockbine, N.A., Bono, J.L. 2012. Association of nucleotide polymorphisms within the O-antigen gene cluster of Escherichia coli O26, O45, O103, O111, O121, and O145 with serogroups and genetic subtypes. Applied and Environmental Microbiology. 78(18):6689-6703.
Biofilm formation by Shiga toxin–producing Escherichia coli O157:H7 and non-O157 strains and their tolerance to sanitizers commonly used in the food processing environment Reprint Icon - (Peer Reviewed Journal)
Wang, R., Bono, J.L., Kalchayanand, N., Shackelford, S.D., Harhay, D.M. 2012. Biofilm formation by Shiga toxin–producing Escherichia coli O157:H7 and non-O157 strains and their tolerance to sanitizers commonly used in the food processing environment. Journal of Food Protection. 75(8):1418-1428.
Phylogeny of Shiga toxin-producing Escherichia coli O157 isolated from cattle and clinically ill humans Reprint Icon - (Peer Reviewed Journal)
Bono, J.L., Smith, T.P., Keen, J.E., Harhay, G.P., McDaneld, T.G., Mandrell, R.E., Jung, W., Besser, T.E., Gerner-Smidt, P., Bielaszewska, M., Karch, H., Clawson, M.L. 2012. Phylogeny of Shiga toxin-producing Escherichia coli O157 isolated from cattle and clinically ill humans. Molecular Biology and Evolution. 29(8):2047-2062. doi: 10.1093/molbev/mss072.
Application of combined SMRT and long-read pyrosequencing to produce reference genome sequences of bacteria associated with respiratory disease outbreaks in beef cattle - (Abstract Only)
Harhay, G.P., Mcvey, D.S., Kitzmiller, A., Clamp, M., Osborne, B., Dwan, C., Zhang, J., Sisneros, N., Wang, S., Peluso, P., Bono, J.L., Chitko Mckown, C.G., Clawson, M.L., Heaton, M.P., Harhay, D.M., Smith, T.P.L. [abstract]. 2012. Application of combined SMRT and long-read pyrosequencing to produce reference genome sequences of bacteria associated with respiratory disease outbreaks in beef cattle. Advances in Genome Biology and Technology, February 15-18, 2012, Marco Island, Florida. Poster No. 129.
Evolution of Shiga toxin-producing Escherichia coli O157: eight major lineages of human and cattle origin strain signature genotypes - (Abstract Only)
Clawson, M.L., Bono, J.L. 2011. Evolution of Shiga toxin-producing Escherichia coli O157: eight major lineages of human and cattle origin strain signature genotypes [abstract]. Proceedings of the BIT's 1st Annual World Congress of Microbes - 2011, July 30 - August 1, 2011, Beijing, China. p. 195.
Evaluation of pyrosequencing for assembly of plasmids carrying IS elements - (Abstract Only)
Chen, C., Lindsey, R.L., Strobaugh Jr, T.P., Frye, J.G., Bono, J.L., Smith, T.P., Meinersmann, R.J. 2011. Evaluation of pyrosequencing for assembly of plasmids carrying IS elements [abstract]. 111th ASM General Meeting.
Virulence-associated and antibiotic resistance genes of microbial populations in cattle feces analyzed using a metagenomic approach Reprint Icon - (Peer Reviewed Journal)
Durso, L.M., Harhay, G.P., Bono, J.L., Smith, T.P. 2011. Virulence-associated and antibiotic resistance genes of microbial populations in cattle feces analyzed using a metagenomic approach. Journal of Microbiological Methods. 84: 278-282.
Bacterial community analysis of beef cattle feedlots reveals that pen surface is distinct from feces - (Peer Reviewed Journal)
Durso, L.M., Harhay, G.P., Smith, T.P., Bono, J.L., Desantis, T.Z., Clawson, M.L. 2011. Bacterial community analysis of beef cattle feedlots reveals that pen surface is distinct from feces. Foodborne Pathogens and Disease. 8(5):647-649. Available: DOI: 10.1089/fpd.2010.0774.
Distribution of Shiga-Toxigenic Escherichia coli O157 in the Gastrointestinal Tract of Naturally O157-Shedding Cattle at Necropsy Reprint Icon - (Peer Reviewed Journal)
Keen, J.E., Laegreid, W.W., Chitko Mckown, C.G., Durso, L.M., Bono, J.L. 2010. Distribution of Shiga-Toxigenic Escherichia coli O157 in the Gastrointestinal Tract of Naturally O157-Shedding Cattle at Necropsy. Applied and Environmental Microbiology. 76(15):5278-5281.
Molecular analysis of plasmid encoded multi-drug resistance (MDR) in Salmonella enterica animal isolates by PFGE, replicon typing, and DNA microarray screening followed by high-throughput DNA sequencing - (Abstract Only)
Lindsey, R.L., Frye, J.G., Bono, J.L., Smith, T.P., Harhay, D.M., Cray, P.J., Meinersmann, R.J. 2010. Molecular analysis of plasmid encoded multi-drug resistance (MDR) in Salmonella enterica animal isolates by PFGE, replicon typing, and DNA microarray screening followed by high-throughput DNA sequencing. American Society for Microbiology. June 8-11, 2010. Toronto, Canada. B16. p41-42.
Animal-to-Animal Variation in Fecal Microbial Diversity among Beef Cattle - (Peer Reviewed Journal)
Durso, L.M., Harhay, G.P., Smith, T.P.L., Bono, J.L., Desantis, T.Z., Harhay, D.M., Andersen, G.L., Keen, J.E., Laegreid, W.W., Clawson, M.L. 2010. Animal-to-Animal Variation in Fecal Microbial Diversity among Beef Cattle. Applied and Environmental Microbiology. 76(14):4858-4862.
Utilization of evolutionary model, bioinformatics and heuristics for development of a multiplex Escherichia coli O157:H7 PCR assay - (Abstract Only)
Burns, F.R., Bono, J.L. 2010. Utilization of evolutionary model, bioinformatics and heuristics for development of a multiplex Escherichia coli O157:H7 PCR assay [abstract]. International Association for Food Protection. 2010:27.
Comparison of cattle fecal communities from animals fed diets containing corn and distiller's grains - (Abstract Only)
Durso, L.M., Harhay, G.P., Wells, J., Bono, J.L., Riethoven, J.M., Shackelford, S.D., Wheeler, T.L., Smith, T.P. 2010. Comparison of cattle fecal communities from animals fed diets containing corn and distiller's grains. PS.25.018, Abstract #393A. Abstract for Microbial Ecology International Symposium, Seattle, WA, Aug. 22-27, 2010. Abstract Book, Pg. 128
Outbreak Investigation: STEC O157 Reprint Icon - (Peer Reviewed Journal)
Durso, L., Maguire, H., Cronquist, A., Bono, J.L. 2010. Outbreak Investigation: STEC O157. Medical Laboratory Observer [serial online]. Available: www.mlo-online.com.
Diverse Genetic Markers Concordantly Identify Bovine Origin Escherichia coli O157 Genotypes Underrepresented in Human Disease Reprint Icon - (Peer Reviewed Journal)
Whitworth, J., Zhang, Y., Bono, J., Pleydell, E., French, N., Besser, T. 2010. Diverse Genetic Markers Concordantly Identify Bovine Origin Escherichia coli O157 Genotypes Underrepresented in Human Disease. Applied and Environmental Microbiology. 76(1):361-365.
Phylogenetic Classification of Escherichia coli O157:H7 Strains of Human and Bovine Origin Using a Novel Set of Nucleotide Polymorphisms Reprint Icon - (Peer Reviewed Journal)
Clawson, M.L., Keen, J.E., Smith, T.P., Durso, L.M., Mcdaneld, T.G., Mandrell, R.E., Davis, M.A., Bono, J.L. 2009. Phylogenetic Classification of Escherichia coli O157:H7 Strains of Human and Bovine Origin Using a Novel Set of Nucleotide Polymorphisms. Genome Biology [serial online]. 10:R56. Available: http://genomebiology.com/2009/10/5/R56.
Genotyping Escherichia coli O157:H7 for Its Ability to Cause Disease in Humans Reprint Icon - (Peer Reviewed Journal)
Bono, J.L. 2009. Genotyping Escherichia coli O157:H7 for Its Ability to Cause Disease in Humans. Current Protocols in Microbiology. 14:5A.3.1-5A.3.10.
Phylogenetic Classification of Escherichia coli O157:H7 Isolates of Human and Bovine Origin Using a Novel Set of Nucleotide Polymorphisms - (Abstract Only)
Clawson, M.L., Keen, J.E., Smith, T.P., Durso, L.M., McDaneld, T.G., Mandrell, R.E., Davis, M.A., Bono, J.L. 2009. Phylogenetic Classification of Escherichia coli O157:H7 Isolates of Human and Bovine Origin Using a Novel Set of Nucleotide Polymorphisms [abstract]. American Society for Microbiology. Abstract No. Z-006. p. 612. 2009 CDROM.
Bacterial Communities Associated with Shiga-Toxigenic E. coli O157:H7 (STEC O157) in Beef Cattle Feedlot Environments - (Abstract Only)
Durso, L.M., Harhay, G.P., Bono, J.L., Smith, T.P., Laegreid, W.W., Keen, J.E., Clawson, M.L. 2009. Bacterial Communities Associated with Shiga-Toxigenic E. coli O157:H7 (STEC O157) in Beef Cattle Feedlot Environments [abstract]. Microbial Ecology 57(3):569.
Escherichia coli O157:H7 and Salmonella in Small Processing Plants and the Effect of Feeding Wet Distillers Grains with Solubles on E. coli O157:H7 - (Abstract Only)
Bosilevac, J.M., Wells, J., Arthur, T.M., Kalchayanand, N., Shackelford, S.D., Berry, E.D., Bono, J.L., Harhay, D.M., Wheeler, T.L., Koohmaraie, M. 2009. Escherichia coli O157:H7 and Salmonella in Small Processing Plants and the Effect of Feeding Wet Distillers Grains with Solubles on E. coli O157:H7 [abstract]. NCBA Beef Industry Safety Summit, San Diego, CA, March 4-6, 2009.
Prevalence and Enumeration of Escherichia coli O157:H7 and Salmonella in U.S. Abattoirs That Process Fewer Than 1,000 Head of Cattle Per Day - (Peer Reviewed Journal)
Bosilevac, J.M., Arthur, T.M., Bono, J.L., Brichta-Harhay, D.M., Kalchayanand, N., King, D.A., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence and Enumeration of Escherichia coli O157:H7 and Salmonella in U.S. Abattoirs That Process Fewer Than 1,000 Head of Cattle Per Day. Journal of Food Protection. 72(6):1272-1278.
International Comparison of Clinical, Bovine, and Environmental Escherichia coli O157 Isolates on the Basis of Shiga Toxin-Encoding Bacteriophage Insertion Site Genotypes Reprint Icon - (Peer Reviewed Journal)
Whitworth, J.H., Fegan, N., Keller, J., Gobius, K.S., Bono, J.L., Call, D.R., Hancock, D.D., Besser, T.E. 2008. International Comparison of Clinical, Bovine, and Environmental Escherichia coli O157 Isolates on the Basis of Shiga Toxin-Encoding Bacteriophage Insertion Site Genotypes. Applied and Environmental Microbiology. 74(23):7447-7450.
Comparison of effects of antimicrobial interventions on multidrug-resistant Salmonella, susceptible Salmonella, and Escherichia coli O157:H7 - (Peer Reviewed Journal)
Arthur, T.M., Kalchayanand, N., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Bono, J.L., Wheeler, T.L., Koohmaraie, M. 2008. Comparison of effects of antimicrobial interventions on multidrug-resistant Salmonella, susceptible Salmonella, and Escherichia coli O157:H7. Journal of Food Protection 71(11):2177-2181.
Microbial Community Structure of Feedlot Cattle Feces - (Abstract Only)
Durso, L.M., Harhay, G.P., Clawson, M.L., Laegreid, W.W., Bono, J.L., Keen, J.E., Smith, T.P. Microbial community structure of feedlot cattle feces. In: Proceedings of the 108th General Meeting of the American Society for Microbiology, June 1-5, 2008, Boston, Massachusetts. 2008 CDROM.
Association of Escherichia coli O157:H7 tir polymorphisms in human infection Reprint Icon - (Peer Reviewed Journal)
Bono, J.L., Keen, J.E., Clawson, M.L., Durso, L.M., Heaton, M.P., Laegreid, W.W. 2007. Association of Escherichia coli O157:H7 tir polymorphisms with human infection. BMC Infectious Diseases. 7:98.
Molecular Serotyping of Escherichia coli O111:H8 - (Peer Reviewed Journal)
Durso, L.M., Bono, J.L., Keen, J.E. 2007. Molecular Serotyping of Escherichia coli O111:H8. Journal of Microbiological Methods. 69:381-383.
Greater Diversity of Shiga Toxin-Encoding Bacteriophage Insertion Sites among Eschericha Coli O157:h7 Isolates from Cattle Than in Those from Humans - (Peer Reviewed Journal)
Besser, T.E., Shaikh, N., Holt, N.J., Tarr, P.I., Konkel, M.E., Malik-Kale, P., Walsh, C.W., Whittam, T.S., Bono, J.L. 2007. Greater diversity of shiga toxin-encoding bacteriophage insertion sites among Eschericha coli O157:H7 isolates from cattle than from humans. Applied and Environmental Microbiology. 73(3):671-679.
Oral neomycin therapy reduces shiga-toxigenic E. coli (STEC) O157 fecal shedding in naturally-infected beef cattle - (Abstract Only)
Keen, J.E., Durso, L.M., Bono, J.L., Laegreid, W.W. 2006. Oral neomycin therapy reduces shiga-toxigenic E. coli (STEC) O157 fecal shedding in naturally-infected beef cattle [abstract]. 2006 Conference of Research Workers in Animal Disease. p.148.
Discrimination of Escherichia Coli O157:h7 Isolates by Genotyping Single Nucleotide Polymorphisms - (Abstract Only)
Bono, J.L., Keen, J.E., Laegreid, W.W. 2006. Discrimination of Escherichia coli O157:H7 isolates by genotyping single nucleotide polymorphisms [abstract]. Proceedings of the 6th International Symposium on Shiga Toxin (Verocytotoxin)--Producing Escherichia coli Infections, October 29 - November 1, 2006, Melbourne, Australia. Poster No. P13.1.07.
Association of Specific Per and Flhd Alleles in Escherichia Coli O157:h7 with Sorbitol Fermentation - (Abstract Only)
Bono, J.L., Keen, J.E., Laegreid, W.W. 2006. Association of specific per and flhd alleles in Escherichia coli O157:H7 with sorbitol fermentation [abstract]. American Society of Microbiologists. Poster No. C-056.
Shiga Toxin Bacteriophage Insertion Sites Identify Diverse Escherihica Coli O157:h7 Strain Types with Distributions Biased to the Bovine Host Reservoir - (Abstract Only)
Besser, T.E., Bono, J.L., Shaikh, N., Tarr, P.I. 2006. Shiga toxin bacteriophage insertion sites identify diverse Escherihica coli O157:H7 strain types with distributions biased to the bovine host reservoir [abstract]. American Society of Microbiologists. Poster No. Z-017.
Shiga-Toxigenic Escherichia Coli O157 in Agricultural Fair Livestock, United States - (Peer Reviewed Journal)
Keen, J.E., Wittum, T.E., Dunn, J.R., Bono, J.L., Durso, L.M. 2006. Shiga-toxigenic Escherichia coli O157 in agricultural fair livestock, United States. Emerging Infectious Diseases 12(5):780-786.
Molecular Serotyping of Escherichia Coli O26:h11 - (Peer Reviewed Journal)
Durso, L.M., Bono, J.L., Keen, J.E. 2005. Molecular serotyping of Escherichia coli O26:H11. Applied and Environmental Microbiology. 71(8):4941-4944.
Discrimintion of Escherichia Coli O157:h7 Isolates by Genotyping Single Nucleotide Polymorphisms - (Abstract Only)
Bono, J.L., Keen, J.E., Clawson, M.L., Heaton, M.P., Chitko Mckown, C.G., Miller, L.C., Laegreid, W.W. 2005. Discrimintion of Escherichia coli O157:H7 isolates by genotyping single nucleotide polymorphisms. [abstract] American Society of Microbiologists. p. 318.
Multiplex Pcr for Serotyping Escherichia Coli O26:h11 - (Abstract Only)
Durso, L.M., Bono, J.L., Keen, J.E. 2004. Multiplex PCR for serotyping Escherichia coli O26:H11 [abstract]. Research Workers in Animal Diseases Conference. p. 121.
Interferon Type I Response in Porcine Reproductive and Respiratory Syndrome Virus-Infected Marc-145 Cells - (Peer Reviewed Journal)
Miller, L.C., Laegreid, W.W., Bono, J.L., Chitko McKown, C.G., Fox, J.M. 2004. Interferon type I response in porcine reproductive and respiratory syndrome virus-infected MARC-145 cells. Archives of Virology. 149(12):2453-2463.
Early Innate Immune Response in Porcine Reproductive and Respiratory Syndrome Virus-Infected Marc-145 Cells - (Abstract Only)
Miller, L.C., Fox, J.M., Bono, J.L., Chitko Mckown, C.G., Laegreid, W.W. 2004. Early innate immune response in porcine reproductive and respiratory syndrome virus-infected marc-145 cells [abstract]. 7th Int. Symp. Positive Strand RNA Viruses. Paper No. P2-G1.
Discrimination of Escherichia Coli O157:h7 from Other Bacteria by Genotyping Single Nucleotide Polymorphisms - (Abstract Only)
Bono, J.L., Keen, J.E., Clawson, M.L., Heaton, M.P., Fox, J.M., Chitko McKown, C.G., Laegreid, W.W. 2004. Discrimination of Escherichia coli O157:H7 from other bacteria by genotyping single nucleotide polymorphisms [abstract]. American Society for Microbiology. Paper No. C-311.
Evaluation of a Real-Time Pcr Kit for Detecting Escherichia Coli O157 in Bovine Fecal Samples - (Peer Reviewed Journal)
BONO, J.L., KEEN, J.E., MILLER, L.C., FOX, J.M., CHITKO MCKOWN, C.G., HEATON, M.P., LAEGREID, W.W. EVALUATION OF A REAL-TIME PCR KIT FOR DETECTING ESCHERICHIA COLI O157 IN BOVINE FECAL SAMPLES. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2004. V. 70. P. 1855-1857.
Gene Expression Profiling of Bovine Macrophages in Response to Escherichia Coli O157:h7 Lipopolysaccharide - (Abstract Only)
CHITKO MCKOWN, C.G., FOX, J.M., MILLER, L.C., HEATON, M.P., BONO, J.L., KEEN, J.E., GROSSE, W.M., LAEGREID, W.W. GENE EXPRESSION PROFILING OF BOVINE MACROPHAGES IN RESPONSE TO ESCHERICHIA COLI O157:H7 LIPOPOLYSACCHARIDE. IMMUNOLOGY RESEARCH WORKSHOP. 2003. ABSTRACT NO. 23.
Fecal Shedding of Shiga-Toxigenic E. Coli (Stec) O157 by Livestock at Agricultural Fairs in the United States - (Abstract Only)
KEEN, J.E., WITTUM, T.E., DUNN, J.R., BONO, J.L., HANSEN, G.E., FONTENOT, M.E. FECAL SHEDDING OF SHIGA-TOXIGENIC E. COLI (STEC) O157 BY LIVESTOCK AT AGRICULTURAL FAIRS IN THE UNITED STATES. RESEARCH WORKERS IN ANIMAL DISEASES CONFERENCE PROCEEDINGS. 2003. ABSTRACT NO. 62.
Gene Expression Profiling of Bovine Macrophages in Response to Escherichia Coli O157:h7 Lipopolysaccharide - (Peer Reviewed Journal)
CHITKO MCKOWN, C.G., FOX, J.M., MILLER, L.C., HEATON, M.P., BONO, J.L., KEEN, J.E., GROSSE, W.M., LAEGREID, W.W. GENE EXPRESSION PROFILING OF BOVINE MACROPHAGES IN RESPONSE TO ESCHERICHIA COLI O157:H7 LIPOPOLYSACCHARIDE. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY. 2004. V. 28. P. 635-645.
Shedding of Salmonella Spp by Livestock at Agricultural Fairs - (Abstract Only)
WITTUM, T.E., KEEN, J.E., HANSEN, G.E., MOLLENKOPF, D.F., FUNK, J.A., DUNN, J.R., BONO, J.L., FONTENOT, M.E. SHEDDING OF SALMONELLA SPP BY LIVESTOCK AT AGRICULTURAL FAIRS. RESEARCH WORKERS IN ANIMAL DISEASES CONFERENCE PROCEEDINGS. 2003. ABSTRACT NO. 61.
Identification of Responsive Genes in Prrsv-Infected Pulmonary Macrophages Using Semi-Quantitative Real-Time Reverse Transcription Pcr (Rt-Pcr) - (Abstract Only)
MILLER, L.C., CHITKO MCKOWN, C.G., BONO, J.L., LAEGREID, W.W., FOX, J.M. IDENTIFICATION OF RESPONSIVE GENES IN PRRSV-INFECTED PULMONARY MACROPHAGES USING SEMI-QUANTITATIVE REAL-TIME REVERSE TRANSCRIPTION PCR (RT-PCR). RESEARCH WORKERS IN ANIMAL DISEASES CONFERENCE PROCEEDINGS. 2003. ABSTRACT NO. 155.
Effect of Exogenous Glucocorticoids and Dietary Change on Winter and Summer Stec O157 Fecal Shedding in Naturally-Infected Beef Cattle - (Abstract Only)
KEEN, J.E., LAEGREID, W.W., CHITKO MCKOWN, C.G., BONO, J.L., FOX, J.M., CLAWSON, M.L., HEATON, M.P. EFFECT OF EXOGENOUS GLUCOCORTICOIDS AND DIETARY CHANGE ON WINTER AND SUMMER STEC O157 FECAL SHEDDING IN NATURALLY-INFECTED BEEF CATTLE. RESEARCH WORKERS IN ANIMAL DISEASES CONFERENCE PROCEEDINGS. 2003. ABSTRACT NO. 83.
Association of Nos2a Haplotypes and Bactericidal Activity of Bovine Monocyte-Derived Macrophages - (Abstract Only)
CHITKO MCKOWN, C.G., HEATON, M.P., GROSSE, W.M., KEEN, J.E., BONO, J.L., CLAWSON, M.L., FOX, J.M., MILLER, L.C., LAEGREID, W.W. ASSOCIATION OF NOS2A HAPLOTYPES AND BACTERICIDAL ACTIVITY OF BOVINE MONOCYTE-DERIVED MACROPHAGES. AMERICAN ASSOCIATION OF IMMUNOLOGISTS PROCEEDINGS. 2003. Abstract No. 150.1.
Single Nucleotide Polymorphisms in the Rfb Locus of Escherichia Coli O157 Stec and Non-Stec Isolates - (Abstract Only)
BONO, J.L., KEEN, J.E., LAEGREID, W.W. SINGLE NUCLEOTIDE POLYMORPHISMS IN THE RFB LOCUS OF ESCHERICHIA COLI O157 STEC AND NON-STEC ISOLATES. INTERNATIONAL SYMPOSIUM AND WORKSHOP ON SHIGA TOXIN ... ESCHERICHIA COLI. 2003. Abstract No. P77.
Sequence Diversity of Escherichia Coli O157:h7 Isolates - (Abstract Only)
LAEGREID, W.W., BONO, J.L., CLAWSON, M.L., KEEN, J.E. SEQUENCE DIVERSITY OF ESCHERICHIA COLI O157:H7 ISOLATES. INTERNATIONAL SYMPOSIUM AND WORKSHOP ON SHIGA TOXIN ... ESCHERICHIA COLI. 2003. Abstract p. 137.
Occurrence of Stec O157, O111, and O26 in Livestock at Agricultural Fairs in the United States - (Abstract Only)
KEEN, J.E., WITTUM, T.E., DUNN, J.R., BONO, J.L., FONTENOT, M.E. OCCURRENCE OF STEC O157, O111, AND O26 IN LIVESTOCK AT AGRICULTURAL FAIRS IN THE UNITED STATES. INTERNATIONAL SYMPOSIUM AND WORKSHOP ON SHIGA TOXIN ... ESCHERICHIA COLI. 2003. Abstract No. O-10.
Detection of E. Coli O157 in Bovine Feces Using the R.A.P.I.D. System E. Coli O157 Detection Kit - (Abstract Only) - (22-Feb-02)
Genomic Sample Sequencing of Three Shiga-Toxigenic E. Coli (Stec) Isolated from Bovine Feces - (Abstract Only) - (12-Dec-01)
Genomic Sample Sequencing of Three Bovine Shiga-Toxigenic E. Coli (Stec) O157, O111 and O26 - (Abstract Only) - (21-Sep-01)
Last Modified: 3/25/2010
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