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United States Department of Agriculture

Agricultural Research Service

Related Topics

Carlos E Suarez
Animal Diseases Research
Research Molecular Biologist

Phone: (509) 335-6341
Fax: (509) 335-8328
305 BUSTAD HALL,WSU
PULLMAN, WA, 99164-6630

Projects
Pharmacological and Immunologic Interventions Against Vector-Borne Bovine and Equine Babesiosis
Appropriated (D)
  Accession Number: 422175

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Targeted surface expression of an exogenous antigen in stably transfected babesia bovis - (Peer Reviewed Journal)
Laughery, J., Knowles Jr, D.P., Schneider, D.A., Mcelwain, T., Suarez, C.E. 2014. Targeted surface expression of an exogenous antigen in stably transfected babesia bovis. PLoS One. DOI:10.1371/journal.pone.0097890.
The glycosylphosphatidylinositol-anchored protein repertoire of babesia bovis and its significance for erythrocyte invasion - (Peer Reviewed Journal)
Rodriguez, A., Florin-Christensen, M., Flores, D., Suarez, C.E., Schnittger, L. 2014. The glycosylphosphatidylinositol-anchored protein repertoire of babesia bovis and its significance for erythrocyte invasion. Ticks and Tick Borne Diseases. 5(3):343-348.
Differential expression of three members of the multidomain adhesion CCp family in babesia bigemina, babesia bovis and theileria equi - (Peer Reviewed Journal)
Bastos, R., Suarez, C.E., Laughery, J., Johnson, W.C., Ueti, M.W., Knowles Jr, D.P. 2013. Differential expression of three members of the multidomain adhesion CCp family in babesia bigemina, babesia bovis and theileria equi. PLoS One. Plos ONE 8(7): e67765.
Repertoire of theileria equi antigens bound by equine antibody during persistent phase of infection - (Peer Reviewed Journal)
Gomes Da Silva, M., Graca, T., Suarez, C.E., Knowles Jr, D.P. 2013. Repertoire of theileria equi antigens bound by equine antibody during persistent phase of infection. Molecular and Biochemical Parasitology. 188(2):109-115.
Evaluation of the growth-inhibitory effect of trifluralin analogues on in vitro cultured babesia bovis parasites - (Peer Reviewed Journal)
Gomes Da Silva, M., Domingos, A., Esteves, M.A., Antunes, S., Cruz, M.M., Suarez, C.E. 2013. Evaluation of the growth-inhibitory effect of trifluralin analogues on in vitro cultured babesia bovis parasites. International Journal for Parasitology. (3):59-68.
Comparative genomic analysis and phylogenetic position of Theileria equi Reprint Icon - (Peer Reviewed Journal)
Kappmeyer, L.S., Thiagarajan, M., Herndon, D.R., Ramsay, J.D., Caler, E., Djikeng, A., Gillespie, J.J., Lau, A.O., Roalson, E.H., Silva, J.C. 2012. Comparative genomic analysis and phylogenetic position of Theileria equi. Biomed Central (BMC) Genomics. BMC Genomics 2012, 13:603.
Acute and persistent infection by a transfected Mo7 strain of Babesia bovis Reprint Icon - (Peer Reviewed Journal)
Suarez, C.E., Laughery, J., Schneider, D.A., Sondgeroth, K.S., Mcelwain, T.F. 2012. Acute and persistent infection by a transfected Mo7 strain of Babesia bovis. Molecular and Biochemical Parasitology. 185(1):52-7.
Characterization of acyl carrier protein and LytB in Babesia bovis apicoplast - (Peer Reviewed Journal)
Caballero, M.C., Pedroni, M.J., Palmer, G.H., Suarez, C.E., Davitt, C., Lau, A.T. 2012. Characterization of acyl carrier protein and LytB in Babesia bovis apicoplast. Molecular and Biochemical Parasitology. (2):125-33.
Expression and strain variation of the novel “Small Open Reading Frame” 3 (smorf) multigene family in Babesia bovis - (Peer Reviewed Journal)
Ferreri, L.M., Brayton, K.A., Sondergoth, K.S., Lau, A.T., Suarez, C.E., Mcelwain, T.F. 2012. Expression and strain variation of the novel “Small Open Reading Frame” 3 (smorf) multigene family in Babesia bovis. International Journal for Parasitology. 42(2):131-8.
A novel neutralization sensitive and subdominant RAP-1-related antigen (RRA) is expressed by babesia bovis merozoites - (Peer Reviewed Journal)
Suarez, C.E., Laughery, J., Bastos, R., Johnson, W.C., Norimine, J., Asenzo, G., Brown, W.C., Florin-Christensen, M., Goff, W.L. 2011. A novel neutralization sensitive and subdominant RAP-1-related antigen (RRA) is expressed by Babesia bovis merozoites. Parasitology. 10.1017/S0031182011000321.
Emerging perspectives in the research of bovine Babesiosis and Anaplasmosis - (Peer Reviewed Journal)
Suarez, C.E., Noh, S.M. 2011. Emerging perspectives in the research of bovine Babesiosis and Anaplasmosis. Veterinary Parasitology. 180(102):109-25.
Bovipain-2, the falcipain-2 ortholog, is expressed in intraerythrocytic stages of the tick-transmitted hemoparasite Babesia bovis - (Peer Reviewed Journal)
Mesplet, M., Echaide, I., Dominguez, M., Mosqueda, J.J., Suarez, C.E., Schnittger, L., Florin-Christensen, M. 2010. Bovipain-2, the falcipain-2 ortholog, is expressed in intraerythrocytic stages of the tick-transmitted hemoparasite Babesia bovis. Parasites & Vectors. 10.1186/1756-3305-3-113.
Babesia bovis expresses Bbo-6cys-E, a member of a novel gene family that is homologous to the 6-cys family of Plasmodium - (Peer Reviewed Journal) - (19-Sep-10)
The novel protein BboRhop68 is expressed by intraerytrhocytic stages of Babesia bovis - (Peer Reviewed Journal) - (26-Jul-10)
One Health Approach to Identify Research Needs in Bovine and Human Babesioses: Workshop Report - (Peer Reviewed Journal)
Perez De Leon, A.A., Strickman, D.A., Knowles Jr, D.P., Fish, D., Thacker, E.L., De La Fuente, J., Krause, P.J., Wikel, S.K., Miller, R., Wagner, G.G., Almazan, C., Hillman, R., Messenger, M.T., Ugstad, P.O., Duhaime, R.A., Teel, P.D., Ortega-Santos, A., Hewitt, D.G., Bowers, E.J., Bent, S.J., Cochran, M.H., Mcelwain, T.F., Scoles, G.A., Suarez, C.E., Davey, R.B., Freeman, J.M., Lohmeyer, K.H., Li, A.Y., Guerrero, F., Kammlah, D.M., Phillips, P.L., Pound, J.M. 2010. One Health approach to identify research needs in bovine and human babesioses: Workshop report. Parasites & Vectors. 3:Article 36.
Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain Reprint Icon - (Peer Reviewed Journal)
Silva, M.G., Ueti, M., Norimine, J., Florin-Christensen, M., Bastos, R., Goff, W.L., Brown, W.C., Oliva, A., Suarez, C.E. 2010. Babesia bovis expresses a neutralization-sensitive antigen that contains a microneme adhesive repeat (MAR) domain. Parasitology International. Available: doi:10.1016/j.parint.2010.03.004
First survey for Babesia bovis and Babesia bigemina infection in cattle from Central and Southern regions of Portugal using serological and DNA detection methods - (Peer Reviewed Journal)
Silva, M., Henriques, G., Sanchez, C., Marques, P.X., Suarez, C.E., Oliva, A. 2009. First survey for Babesia bovis and Babesia bigemina infection in cattle from Central and Southern regions of Portugal using serological and DNA detection methods. Veterinary Parasitology. 166(1-2):66-72.
Babesia bovis: Transcriptional analysis of rRNA gene unit expression Reprint Icon - (Peer Reviewed Journal)
Laughery, J.M., Lau, A.O., White, S.N., Howell, J.M., Suarez, C.E. 2009. Babesia bovis: Transcriptional analysis of rRNA gene unit expression. Experimental Parasitology. 123(1):45-50.
Development of a tandem repeat-based multilocus typing system distinguishing Babesia bovis geographic isolates Reprint Icon - (Peer Reviewed Journal)
Perez-Llaneza, A., Caballero, M., Baravalle, E., Mesplet, M., Mosqueda, J., Suarez, C.E., Echaide, I., Katzer, F., Pacheco, G.M., Florin-Christensen, M., Schnittger, L. 2010. Development of a tandem repeat-based multilocus typing system distinguishing Babesia bovis geographic isolates. Veterinary Parasitology. 167(2-4):196-204.
In silico predicted conserved B-cell epitopes in the Merozoite Surface Antigen -2 family of B. bovis are neutralization-sensitive Reprint Icon - (Peer Reviewed Journal)
Dominguez, M., Echaide, I., Torioni De Echaide, S., Mosqueda, J., Cetra, B., Suarez, C.E., Florin-Christensen, M. 2010. In silico predicted conserved B-cell epitopes in the Merozoite Surface Antigen -2 family of B. bovis are neutralization-sensitive. Veterinary Parasitology. 167(2-4):216-226.
Transfection systems for Babesia bovis: a review of methods for the transient and stable expression of exogenous genes Reprint Icon - (Peer Reviewed Journal)
Suarez, C.E., Mcelwain, T.F. 2010. Transfection systems for Babesia bovis: a review of methods for the transient and stable expression of exogenous genes. Veterinary Parasitology. 167(2-4):205-215.
Stable expression of a GFP-BSD fusion protein in Babesia bovis merozoites Reprint Icon - (Peer Reviewed Journal)
Suarez, C.E., Mcelwain, T. 2009. Stable expression of a GFP-BSD fusion protein in Babesia bovis merozoites. International Journal for Parasitology. 39(3):289-297.
An impedance spectroscopy method for the detection and evaluation of Babesia bovis antibodies in cattle Reprint Icon - (Peer Reviewed Journal)
Silva, M., Helali, S., Esseghaierb, C., Suarez, C.E., Oliva, A., Abdelghani, A. 2008. An impedance spectroscopy method for the detection and evaluation of Babesia bovis antibodies in cattle. Sensors and Actuators B: Chemical. 135:206-213.
Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle Reprint Icon - (Peer Reviewed Journal)
Goff, W.L., Johnson, W.C., Molloy, J.B., Jorgensen, W.K., Waldron, S.J., Figueroa, J.V., Matthee, O., Adams, D.S., Mcguire, T.C., Pino, I., Mosqueda, J., Palmer, G.H., Suarez, C.E., Knowles Jr, D.P., Mcelwain, T.F. 2008. Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle. Clinical and Vaccine Immunology. 15(9):1316-1321.
Molecular Characterization of Babesia bovis Strains Using PCR Restriction Fragment Length Polymorphism Analysis of the msa2-a/b Genes Reprint Icon - (Peer Reviewed Journal)
Wilcowsky, S., Farber, M., Gil, G., Echaide, I., Mosqueda, J., Alcaraz, E., Suarez, C.E., Florin-Christensen, M. 2008. Molecular Characterization of Babesia bovis strains using PCR restriction length polymorphism analysis of the msa2s/b genes. Annals of the New York Academy of Sciences. 1149:141-144.
Transient transfection of purified Babesia bovis merozoites - (Peer Reviewed Journal)
Suarez, C.E., Mcelwain, T.F. 2008. Transient transfection of purified Babesia bovis merozoites. Experimental Parasitology. 118(4):498-504.
Genome Sequence of Babesia bovis and Camparative Analysis of Apicomplexan Hemoprotozoa - (Peer Reviewed Journal)
Brayton, K., Lau, A.O., Herndon, D.R., Hannick, L., Kappmeyer, L.S., Berens, S.J., Bidwell, S.L., Brown, W.C., Crabtree, J., Fadrosh, D., Feldblum, T., Forberger, H.A., Haas, B.J., Howell, J.M., Khouri, H., Koo, H., Mann, D.J., Norimine, J., Paulsen, I.T., Radune, D., Ren, Q., Smith, R.K., Suarez, C.E., White, O., Wortman, J.R., Knowles Jr, D.P., Mcelwain, T.F., Nene, V.M. 2007. Genome Sequence of Babesia bovis and Camparative Analysis of Apicomplexan Hemoprotozoa. PLoS Pathogens. 3(10):1401-1413.
Evidence for a relationship between bovine erythrocyte lipid membrane peculiarities and immune pressure from ruminal ciliates Reprint Icon - (Peer Reviewed Journal)
Gimenez, G., Florin-Christensen, M., Belaunzaran, M.L., Isola, E.L., Suarez, C.E., Florin-Christensen, J. 2007. Evidence for a relationship between bovine erythrocyte lipid membrane peculiarities and immune pressure from ruminal ciliates. Veterinary Immunology and Immunopathology. 119(3-4):171-179.
Stable expression of a GFP-BSD fusion protein in transfected and blasticidin-selected B. bovis merozoites - (Abstract Only)
Suarez, C.E., Mcelwain, T.F. 2007. Stable expression of a GFP-BSD fusion protein in transfected and blasticidin-selected B. bovis merozoites. Molecular Parasitology Meeting XVIII, September 16-20, Woods Hole, MA.
Optimizacion of Babesia bovis transfection methods - (Monograph)
Suarez, C.E., Laughery, J., Gonzalez, M.G., Mcelwain, T. 2007. Optimizacion of Babesia bovis transfection methods. Parassitologia. 49(Suppl. 1):67-70.
Taking advantage of the polymorphism of the MSA-2 family for Babesia bovis strain characterization - (Monograph)
Wilkowsky, S., Farber, M., Echaide, I., Mosqueda, J., Alcaraz, E., Suarez, C.E., Florin-Christensen, M. 2007. Taking advantage of the polymorphism of the MSA-2 family for Babesia bovis strain characterization. Parassitologia. 49(Suppl. 1):63-66.
Search for Babesia bovis vaccine candidates - (Monograph)
Florin-Christensen, M., Schnittger, L., Dominguez, M., Mesplet, M., Rodriguez, A., Ferreri, L., Asenzo, G., Wilkowsky, S., Farber, M., Echaide, I., Suarez, C.E. 2007. Search for Babesia bovis vaccine candidates. Parassitologia. 49(Suppl. 1):9-12.
Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Babesia bovis - (Peer Reviewed Journal)
Goff, W.L., Molloy, J.B., Johnson, W.C., Suarez, C.E., Pino, I., Rhalem, A., Sahibi, H., Ceci, L., Carelli, G., Adams, D.S., Mcguire, T.C., Knowles Jr, D.P., Mcelwain, T.F. 2006. Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Babesia bovis. Clinical and Vaccine Immunology. 13(11):1212-1216.
Characterization and Gene Expression of Babesia Bovis Elongation Factor-1alpha - (Peer Reviewed Journal)
Suarez, C.E., Norimine, J., Lacy, P.A., Mcelwain, T.F. 2006. Characterization and gene expression of Babesia bovis elongation factor-1alpha. International Journal for Parasitology. 36(8):965-973.
Prospects for Recombinant Vaccines Against Bovine Babesiosis. - (Peer Reviewed Journal)
Brown, W.C., Norimine, J., Goff, W.L., Suarez, C.E., Mcelwain, T.F. 2006. Prospects for recombinant vaccines against bovine babesiosis. 2006. Parasite Immunology. 28:315-327.
Fewer Prpc Myeloid-Based Cells in Sheep with the Prion Resistant Genotype - (Peer Reviewed Journal)
Hoesing, L.M., Baszler, T.V., O'Rourke, K.I., Suarez, C.E., Bakko, M., Alverson, J., Knowles, Jr., D.P. 2006. Fewer PrPc myeloid-based cells in sheep with the prion resistant genotype. NeuroReport. 17(2):125-129.
Neospora Caninum: Antibodies Directed Against Tachyzoite Surface Protein Ncsrs2 Inhibit Parasite Attachment and Invasion of Placental Trophoblasts in Vitro - (Peer Reviewed Journal)
Haldorson, G., Stanton, J., Mathison, B.A., Suarez, C.E., Baszler, T. 2006. Neospora caninum: antibodies directed against tachyzoite surface protein ncsrs2 inhibit parasite attachment and invasion of placental trophoblasts in vitro. Experimental Parasitology. 112(3):172-178.
Identification of Vaccine Candidate Peptides in the Ncsrs2 Surface Protein of Neospora Caninum by Using Cd4+ Cytotoxic T Lymphocytes and Gamma Interferon-Secreting T Lymphocytes of Infected Holstein Cattle - (Peer Reviewed Journal)
Staska, L.M., Davies, C.J., Brown, W.C., Mcguire, T.C., Suarez, C.E., Park, J.Y., Mathison, B.A., Abbott, J.R., Baszler, T.V. 2005. Identification of vaccine candidate peptides in the ncsrs2 surface protein of neospora caninum by using cd4+ cytotoxic t lymphocytes and gamma interferon-secreting t lymphocytes of infected holstein cattle. Infection and Immunity. 73(3):1321-1329.
Use of a Monoclonal Antibody Against Babesia Bovis Merozoite Surface Antigen-2c for the Development of a Competitive Elisa Test. - (Proceedings)
Dominguez, M., Zabal, O., Wilkowsky, S., Echaide, I., Torioni, D.E., Asenzo, G., Rodriguez, A., Zamorano, P., Farber, M., Suarez, C.E., Florin-Christensen, M. 2004. Use of a monoclonal antibody against babesia bovis merozoite surface antigen-2c for the development of a competitive elisa test. Annals Of The New York Academy Of Sciences. 1026:165-170.
Intergenic Regions in the Rhoptry Associated Protein-1 (Rap-1) Locus Promote Exogenous Gene Expression in Babesia Bovis. - (Peer Reviewed Journal)
Suarez, C.E., Palmer, G.H., LeRoith, T., Florin-Christensen, M., Crabb, B., McElwain, T.F. 2004. Intergenic regions in the rhoptry associated protein-1 (rap-1) locus promote exogenous gene expression in Babesia bovis. International Journal for Parasitology. 34:1177-1184.
Early Immune Responses in Bovines Experimentally Infected with the Hemoparasite Babesia Bovis - (Abstract Only)
Asenzo, G., Dominguez, M., Rodriguez, A., Echaide, I., Wilkowsky, S., Suarez, C.E., Zamorano, P., Florin-Christensen, M. 2003. Early immune responses in bovines experimentally infected with the hemoparasite babesia bovis [abstract]. Biocell. 27:1-172.
Development of a New System for Babesia Bovis Strain Identification Using Molecular Markers - (Abstract Only)
Wilkowsky, S., Dominguez, M., Farber, M., Echaide, I., Valentini, I., Alcaraz, E., Cetra, B., Suarez, C.E., Florin-Christensen, M. 2003. Development of a new system for babesia bovis strain identification using molecular markers [abstract]. Biocell. 27:1-172.
Development of a Competitive Elisa Test for Babesia Bovis Infections Based on Merozoite Surface Antigen-2c - (Abstract Only)
Dominguez, M., Zabal, O., Wilkowsky, S., Rodriguez, A., Asenzo, G., Farber, M., Echaide, I., Echaide, S.T., Suarez, C.E., Florin-Christensen, M. 2003. Development of a competitive elisa test for babesia bovis infections based on merozoite surface antigen-2c [abstract]. Biocell. 27:1-172.
Stimulation of T-Helper Cell Gamma Interferon and Immunoglobulin G Responses Specific for Babesia Bovis Rhoptry-Associated Protein 1 (Rap-1) Or a Rap-1 Protein Lacking in the Carboxy-Terminal Repeat Region Is Insufficient... - (Peer Reviewed Journal)
Norimine, J., Mosqueda, J., Suarez, C.E., Palmer, G.H., Mcelwain, T.F., Mbassa, G., Brown, W.C. 2003. Stimulation of t-helper cell gamma interferon and immunoglobulin g responses specific for babesia bovis rhoptry-associated protein 1 (rap-1) or a rap-1 protein lacking in the carboxy-terminal repeat region is insufficient to provide protective immunity again virulent B. bovis challange. Infection and Immunity. 2003. 71(9):5021-5032.
Babesia Bovis Merozoite Surface Protein-2c (Msa-2c) Contains Highly Immunogenic, Conserved B-Cell Epitopes That Elicit Neutralization-Sensitive Antibodies in Cattle - (Peer Reviewed Journal) - (26-Dec-02)
Organization, Transcription, and Expression of Rhoptry Associated Protein Genes in the Babesia Bigemina Rap-1 Locus - (Peer Reviewed Journal) - (17-Dec-02)
Competitive Enzyme-Linked Immunosorbent Assay Based on a Rhoptry-Associated Protein 1 Epitope Specifically Identifies Babesia Bovis-Infected Cattle - (Peer Reviewed Journal)
Goff, W.L., McElwain, T.F., Suarez, C.E., Johnson, W.C., Brown, W.C., Norimine, J., Knowles, D.P. Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein-1 epitope specifically identifies Babesia bovis-infected cattle. Clinical and Diagnostic Laboratory Immunology. 2002. v. 10. p. 38-43.
The Babesia Bovis Merozoite Surface Antigen 2 Locus Contains Four Tandemly Arranged and Expressed Genes Encoding Immunologically Distinct Proteins - (Peer Reviewed Journal) - (17-Apr-02)
Immunodominant Epitopes in Babesia Bovis Rhoptry Associated Protein-1 That Elicit Memory Cd4+-T-Lymphocyte Responses in B. Bovis Immune Individuals Are Located in the Amino-Terminal Domain - (Peer Reviewed Journal) - (02-Jan-02)
A Novel 20-Kilodalton Protein Conserved in Babesia Bovis and B.BIGEMINA Stimulates Memory Cd4+t Lymphocyte Responses in B.BOVIS-IMMUNE Cattle - (Peer Reviewed Journal) - (29-Aug-01)
A Unique Phospholipid Organization in Bovine Erythrocyte Membranes - (Peer Reviewed Journal) - (25-Jun-01)
Immunostimulatory Cpg-Modified Plasmid DNA Enhances Il-12, Tnf-a and No Production by Bovine Macrophages - (Peer Reviewed Journal) - (09-Feb-01)
DNA from Protozoan Parasites Babesia Bovis, Trypanosoma Cruzi, and T.BRUCEIIS Mitogenic for B Lymphocytes and Stimulates Macrophage Expression of Interleukin-12, Tumor Necrosis Factor Alpha, and Nitric Oxide - (Peer Reviewed Journal) - (05-Jan-01)
Characterization of Allelic Variation in the Babesia Bovis Merozoite Surface Antigen-1 (Msa-1) Locus and Identification of a Cross-Reactive, Inhibition-Sensitive Msa-1 Epitope - (Peer Reviewed Journal) - (16-Sep-00)
Characterization of Allelic Variation in the Babesia Bovis Merozoite Surface Antigen-1 Locus (Msa-1) - (Proceedings)
Suarez, C.E., Florin-Christense, M., Hines, S., Palmer, G., Brown, W., Mcelwain, T. 2000. Characterization of allelic variation in the babesia bovis merozoite surface antigen-1 locus (msa-1). Conference Research Workers Disease Meeting.
Expression of Polymorphic Msp1b Genes During Acute Anaplasma Marginale Rickettsemia - (Peer Reviewed Journal) - (20-Dec-99)
Phosphatidylcholine Formation Is the Predominant Lipid Biosynthetic Event in the Hemoparasite Babesia Bovis - (Peer Reviewed Journal) - (01-Nov-99)
Last Modified: 10/21/2014