Contact information

Andrew Larack Stone

Andrew.stone@usda.gov

Foreign Disease/Weed Science Research
1301 Ditto Avenue, Fort Detrick, MD 21702
Phone: (301)-619-7309
Fax: (301) 619-2880

Andrew Stone is a support scientist (Biologist) at the Foreign Disease/Weed Science Research Unit, having worked at this Frederick, MD ARS location since 1987. Research topics include risk assessments for the potential US emergence of soybean dwarf virus and Wheat Dwarf Virus, and surveying for and identifying their potential insect vectors in the U.S. Other skills include virus purification, production of anti-sera and monoclonal antibodies and the design and validation of RT-PCR and real time PCR assays for plum pox virus and citrus greening disease.

Currently detailed to Dr. Rachel Koch-Bach’s lab working on the fungal causal agents of soybean red leaf blotch, coffee leaf rust and peanut smut.

Previously worked as a Biological Science Aide (Plants) in the Fruit Lab in Beltsville, MD on meristem culture and virus indexing of strawberry plants while an undergraduate student (1985-1986).

Education
Graduate student, Agronomy and Plant Molecular Virology, University of Maryland 1993-1994
B.S, Biology, University of Maryland 1987
B.A/M.A Romance Languages, University of Chicago, 1976

Publications

• Publications

  • (Clicking on the reprint icon will take you to the publication reprint.)
  • Almond can be infected by Plum Pox Virus-D isolate Penn4 and is a transmission-competent host -(Peer Reviewed Journal)
    Rogers, E.E., Stone, A.L., Burchard, E.A., Sherman, D.J., Dardick, C.D. 2024. Almond can be infected by Plum Pox Virus-D isolate Penn4 and is a transmission-competent host. Plant Disease. https://doi.org/10.1094/PDIS-09-23-1910-SC.
    
  • Global phylogenic analysis of soybean dwarf virus isolates and their associations with aphid vectors and severe disease in soybeans -(Peer Reviewed Journal)
    Stone, A.L., Damsteegt, V.L., Smith, O.P., Stewart, L.R. 2024. Global phylogenic analysis of soybean dwarf virus isolates and their associations with aphid vectors and severe disease in soybeans. Virology. 591:109984. https://doi.org/10.1016/j.virol.2024.109984.
    
  • Viral reservoir capacity of wild Prunus alternative hosts of plum pox virus through multiple cycles of transmission and dormancy -(Peer Reviewed Journal)
    Collum, T.D., Stone, A.L., Sherman, D.J., Damsteegt, V.D., Schneider, W.L., Rogers, E.E. 2022. Viral reservoir capacity of wild Prunus alternative hosts of plum pox virus through multiple cycles of transmission and dormancy. Plant Disease. 106:101-106. 10.1094/PDIS-04-21-0802-RE.
    
  • Identification of phloem translatome alterations during Candidatus Liberibacter asiaticus infection in tolerant and susceptible citrus cultivars -(Abstract Only)
    Collum, T.D., Grando, M., Stone, A.L., Sherman, D.J., Culver, J.N., Dardick, C., Stover, E., Rogers, E.E. 2022. Identification of phloem translatome alterations during Candidatus Liberibacter asiaticus infection in tolerant and susceptible citrus cultivars. American Phytopathological Society Annual Meeting. https://10.1094/PHYTO-111-10-S2.1.
    
  • Dynamic changes impact the plum pox virus population structure during leaf and bud development -(Peer Reviewed Journal)
    Tamukong, Y.B., Collum, T.D., Stone, A.L., Kappagantu, M., Sherman, D.J., Rogers, E.E., Dardick, C.D., Culver, J.N. 2020. Dynamic changes impact the plum pox virus population structure during leaf and bud development. Virology. 548:192-199. https://doi.org/10.1016/j.virol.2020.06.014.
    
  • Reassortment of genome segments creates stable lineages among strains of Orchid fleck virus infecting citrus in Mexico -(Peer Reviewed Journal)
    Roy, A., Stone, A.L., Otero-Colina, G., Wei, G., Brlansky, R., Ochoa, R., Bauchan, G.R., Schneider, W.L., Nakhla, M., Hartung, J.S. 2019. Reassortment of genome segments creates stable lineages among strains of Orchid fleck virus infecting citrus in Mexico. Phytopathology. 110:106-120. https://doi.org/10.1094/PHYTO-07-19-0253-FI.
    
  • Aphid vectors impose a major bottleneck on Soybean dwarf virus populations for horizontal transmission in soybean -(Peer Reviewed Journal)
    Tian, B., Gildow, F.E., Stone, A.L., Sherman, D.J., Damsteegt, V.D., Schneider, W.L. 2019. Aphid vectors impose a major bottleneck on Soybean dwarf virus populations for horizontal transmission in soybean. Phytopathology Research. 1:29. https://doi.org/10.1186/s42483-019-0037-3.
    
  • Translating ribosome affinity purification profiling of plum pox virus (PPV) infected leaf tissues in Prunus domestica L reveals post-dormancy spatial coordination of defense responses in phloem tissues -(Peer Reviewed Journal)
    Collum, T.D., Stone, A.L., Sherman, D.J., Rogers, E.E., Dardick, C.D., Culver, J.N. 2019. Translating ribosome affinity purification profiling of plum pox virus (PPV) infected leaf tissues in Prunus domestica L reveals post-dormancy spatial coordination of defense responses in phloem tissues. Molecular Plant-Microbe Interactions. 33(1) 66-77. https://doi.org/10.1094/MPMI-06-19-0152-FI.
    
  • First report of Hibiscus infecting Cilevirus in Citrus sinensis in Meta and Casanare, Colombia -(Peer Reviewed Journal)
    Roy, A., Stone, A.L., Leon, M.G., Hartung, J.S., Wei, G., Mavrodieva, V., Nakhla, M.K., Schneider, W.L., Brlansky, R.H. 2018. Sweet orange showing leprosis symptoms in Colombia are naturally infected with Hibiscus infecting cilevirus and citrus leprosis virus C2. Plant Disease. https://doi.org/10.1094/PDIS-01-18-0150-PDN.
    
  • Transcriptome amplification coupled with nanopore sequencing as a surveillance tool for plant pathogens in plant and insect tissues -(Peer Reviewed Journal)
    Ribiero-Bronzato, A., Sherman, D.J., Stone, A.L., Wilson, V., Schneider, W.L., King, J. 2018. Transcriptome amplification coupled with nanopore sequencing as a surveillance tool for plant pathogens in plant and insect tissues. Plant Disease. 102:1648-1652. https://doi.org/10.1094/PDIS-04-17-0488-RE.
    
  • First report of Cilevirus associated with green ringspot on senescent hibiscus leaves in Tampa, Florida -(Peer Reviewed Journal)
    Roy, A., Stone, A.L., Melzer, M.J., Hartung, J.S., Mavrodieva, V., Nakhla, M.K., Brlansky, R.H., Schneider, W.L. 2017. First report of Cilevirus associated with green ringspot on senescent hibiscus leaves in Tampa, Florida. Plant Disease. http://doi.org/10.1094/PDIS-11-17-1699-PDN.
    
  • Complete nucleotide sequence of a novel Hibiscus-infecting Cilevirus from Florida and its relationship with closely associated Cileviruses-(Peer Reviewed Journal)
    Roy, A., Stone, A.L., Melzer, M.J., Hartung, J.S., Shao, J.Y., Mavrodieva, V., Nakhla, M.K., Brlansky, R.H., Schneider, W.L. 2018. Complete nucleotide sequence of a novel Hibiscus-infecting Cilevirus from Florida and its relationship with closely associated Cileviruses. Genome Announcements. 6(4):e01521-17.
    
  • Host adaptation of soybean dwarf virus following serial passages on pea (Pisum sativum) and soybean (Glycine max)-(Peer Reviewed Journal)
    Tian, B., Gildow, F.E., Stone, A.L., Sherman, D.J., Damsteegt, V.D., Schneider, W.L. 2017. Host adaptation of soybean dwarf virus following serial passages on pea (Pisum sativum) and soybean (Glycine max). Viruses. 9:155. doi: 10.3390/v9060155..
    
  • Insect E-probe Diagnostic Nucleic acid Analysis (EDNA): the application of a novel bioinformatic tool to detection of vectors and pathogens in individual insect and simulated insect trap metagenomes-(Abstract Only)
    Schneider, W.L., Stone, A.L., Sherman, D.J., Wight-Malapi, M., Andreason, S.A., Daniels, J., Ochoa Corona, F., Crouch, J., Wayadande, A. 2015. Insect E-probe Diagnostic Nucleic acid Analysis (EDNA): the application of a novel bioinformatic tool to detection of vectors and pathogens in individual insect and simulated insect trap metagenomes. Phytopathology. 105: S4:124.
    
  • Identification and molecular characterization of nuclear Citrus leprosis virus, an unassigned Dichorhavirus genus member associated with citrus leprosis disease in Mexico-(Peer Reviewed Journal)
    Roy, A., Stone, A.L., Shao, J.Y., Colina, G., Wei, G., Choudary, N., Achor, D., Nakhla, M., Levy, L., Hartung, J.S., Schneider, W.L., Brlansky, R. 2015. Identification and molecular characterization of nuclear Citrus leprosis virus, an unassigned Dichorhavirus genus member associated with citrus leprosis disease in Mexico. Virus Research. 105:564-575.
    
  • First report of citrus leprosis virus nuclear type in Colombia-(Peer Reviewed Journal)
    Roy, A., Leon, M., Stone, A.L., Schneider, W.L., Hartung, J.S., Brlansky, R. 2014. First report of citrus leprosis virus nuclear type in Colombia. Plant Disease. 98:1162.
    
  • Genome assembly of citurs leprosis virus nuclear type reveals a close association with orchid fleck virus -(Peer Reviewed Journal)
    Roy, A., Choudary, N., Colina, G., Stone, A.L., Shao, J.Y., Levy, L., Nakhla, M., Hollingsworth, C., Hartung, J.S., Schneider, W.L., Brlansky, R. 2013. Genome assembly of citurs leprosis virus nuclear type reveals a close association with orchid fleck virus. Genome Announcements. doi:10.1128/genomeA.00519-13.
    
  • Development of primers and probes for genus and species specific detection of 'Candidatus Liberibacter species' by real-time PCR-(Peer Reviewed Journal)
    Ananthakrishnan, G., Choudhary, N., Roy, A., Sengoda, V.G., Postnikova, E.N., Hartung, J.S., Stone, A.L., Damsteegt, V.D., Schneider, W.L., Munyaneza, J.E., Brlansky, R.H. 2013. Development of primers and probes for genus and species specific detection of 'Candidatus Liberibacter species' by real-time PCR. Plant Disease. 97:1235-1243.
    
  • A previously undescribed potyvirus isolated and characterized from arborescent Brugmansia -(Peer Reviewed Journal)
    Damsteegt, V.D., Stone, A.L., Smith, O.P., Mcdaniel, L., Sherman, D.J., Dardick, C.D., Hammond, J., Jordan, R.L., Schneider, W.L. 2013. A previously undescribed potyvirus isolated and characterized from arborescent Brugmansia. Archives of Virology. 158:1235-1244.
    
  • Molecular analysis of Soybean dwarf virus isolates in the eastern United States confirms the presence of both D and Y strains and provides evidence of mixed infections and recombination-(Peer Reviewed Journal)
    Schneider, W.L., Damsteegt, V.D., Stone, A.L., Kuhlman, M., Bunyard, B., Sherman, D.J., Graves, M., Smythers, G., Smith, O., Hatziloukas, E. 2011. Molecular analysis of Soybean dwarf virus isolates in the eastern United States confirms the presence of both D and Y strains and provides evidence of mixed infections and recombination. Virology. 412: 46-54.
    
  • Molecular, ultrastructural and biological characterization of Pennsylvania isolates of plum pox potyvirus (PPV)-(Peer Reviewed Journal)
    Schneider, W.L., Damsteegt, V.D., Gildow, F.E., Stone, A.L., Sherman, D.J., Levy, L.E., Mavrodieva, V., Richwine, N., Welliver, R., Luster, D.G. 2011. Molecular, ultrastructural and biological characterization of Pennsylvania isolates of plum pox potyvirus (PPV). Phytopathology. 94:528-533.
    
  • Acquistion and transmissibility of United States Soybean dwarf virus isolates by the soybean aphid Aphis glycines-(Peer Reviewed Journal)
    Damsteegt, V.D., Stone, A.L., Kuhlmann, M., Gildow, F.E., Domier, L.L., Sherman, D.J., Tian, B., Schneider, W.L. 2011. Acquistion and transmissibility of United States Soybean dwarf virus isolates by the soybean aphid Aphis glycines. Plant Disease. 95: 945-950.
    
  • Analysis of visual symptomatology in peach and plum inoculated with U.S. PPV isolates-(Abstract Only)
    Younkins, A.A., Stone, A.L., Sherman, D.J., Schneider, W.L., Scorza, R., Damsteegt, V.D. 2010. Analysis of visual symptomatology in peach and plum inoculated with U.S. PPV isolates. Phytopathology. 100:S209.
    
  • The relevance of Murraya paniculata and related species as potential hosts and inoculum reservoirs of Candidatus Liberibacter asiaticus, causal agent of Huanglongbing (HLB) -(Peer Reviewed Journal)
    Damsteegt, V.D., Postnikova, E.N., Stone, A.L., Kuhlmann, M., Wilson, C., Sechler, A.J., Schaad, N.W., Brlansky, R.H., Schneider, W.L. 2010. The relevance of Murraya paniculata and related species as potential hosts and inoculum reservoirs of Candidatus Liberibacter asiaticus, causal agent of Huanglongbing (HLB). Plant Disease. 94:528-533.
    
  • An improved method of DNA extraction from Diaphorina citri for HLB detection-(Abstract Only)
    Postnikova, E.N., Stone, A.L., Wilson, C.M., Sherman, D.J., Sechler, A.J., Schuenzel, E., Schaad, N.W., Schneider, W.L., Damsteegt, V.D. 2009. An improved method of DNA extraction from Diaphorina citri for HLB detection. Phytopathology. 99:S104.
    
  • Preliminary evidence for mixed populations of Ca. Liberibacter species in Huanglongbing infections-(Abstract Only)
    Postnikova, E.N., Stone, A.L., Wilson, C.M., Sherman, D.J., Sechler, A.J., Schuenzel, E., Schaad, N.W., Schneider, W.L., Damsteegt, V.D. 2009. Preliminary evidence for mixed populations of Ca. Liberibacter species in Huanglongbing infections. Phytopathology. 99:S205.
    
  • Cultivation of Candidatus Liberibacter asiaticus and Ca. L. americanus associated with Huanglongbing-(Peer Reviewed Journal)
    Sechler, A.J., Schuenzel, E., Cooke, P.H., Donnua, S., Thaveechai, N., Postnikova, E.N., Stone, A.L., Schneider, W.L., Damsteegt, V.D., Schaad, N.W. 2009. Cultivation of Candidatus Liberibacter asiaticus and Ca. L. americanus associated with Huanglongbing. Phytopathology. 99:480-486.
    
  • IDENTIFICATION OF A MUTATION IN THE PLUM POX POTYVIRUS NIB GENE ASSOCIATED WITH ADAPTATION TO PEA (PISUM SATIVUM)-(Peer Reviewed Journal)
    Wallis, C.M., Schneider, W.L., Stone, A.L., Sherman, D.J., Damsteegt, V.D., Gildow, F.E. 2007. Identification of a mutation in the plum pox potyvirus nib gene associated with adaptation to pea (pisum sativum). Journal of General Virology. 88:2839-2845.
    
  • Murraya paniculata (orange jasmine), a host and possible inoculum reservoir for Candidatus Liberibacter asiaticus, causal agent of Huanglongbing -(Abstract Only)
    Damsteegt, V.D., Schneider, W.L., Stone, A.L. 2007. Murraya paniculata (orange jasmine), a host and possible inoculum reservoir for Candidatus Liberibacter asiaticus, causal agent of Huanglongbing. Phytopathology. 97:S26 . Phytopathology.
    
  • PRUNUS HOST RANGE OF PLUM POX VIRUS (PPV) IN THE UNITED STATES BY APHID AND GRAFT INOCULATION-(Peer Reviewed Journal)
    Damsteegt, V.D., Scorza, R., Stone, A.L., Schneider, W.L., Webb, K.K., Demuth, M.A., Gildow, F.E. 2007. Prunus host range of plum pox virus (ppv) in the United States by aphid and graft inoculation. Plant Disease. 91:18-23
    
  • QUANTIFICATION OF POSITIVE AND NEGATIVE STRAND RATIOS FOR PLUM POX VIRUS BY REAL-TIME FLUORESCENT REVERSE TRANSCRIPTION-PCR-(Peer Reviewed Journal)
    
    
  • IN PLANTA INTERACTIONS OF THREE PLUM POX POTYVIRUS STRAINS WITHIN CULTIVAR LOVELL PEACH SEEDLINGS-(Abstract Only)
    Damsteegt, V.D., Stone, A.L., Sherman, D.J., Schneider, W.L., Gildow, F., Luster, D.G. 2005. In planta interactions of three plum pox potyvirus strains within cultivar Lovell peach seedlings[abstract]. Phytopathology. 95:S22.
    
  • PLUM POX POTYVIRUS POPULATION DIVERSITY IN PENNSYLVANIA, IN SINGLE ORCHARDS, AND IN INDIVIDUAL REPLICATING POPULATIONS-(Other)
    Schneider, W.L., Sherman, D.J., Stone, A.L., Buckley, K., Damsteegt, V.D. 2005. Plum pox potyvirus population diversity in Pennsylvania, in single orchards, and in individual replicating populations [abstract]. Phytopathology. 95:S94.
    
  • THE SOYBEAN APHID, APHIS GLYCINES, A NEW VECTOR OF ENDEMIC DWARFING AND YELLOWING ISOLATES OF SOYBEAN DWARF LUTEOVIRUS-(Abstract Only)
    Damsteegt, V.D., Stone, A.L., Schneider, W.L., Sherman, D.J., Gildow, F.E., Luster, D.G. 2005. The soybean aphid, Aphis glycines, a new vector of endemic dwarfing and yellowing isolates of soybean dwarf luteovirus [abstracr]. Phytopathology. 95:S22.
    
  • TRANSMISSION OF TWO NORTH AMERICAN ISOLATES OF PLUM POX VIRUS: IDENTIFICATION OF APHID VECTORS AND SPECIES-SPECIFIC TRANSMISSION FROM INFECTED STONE FRUITS-(Proceedings)
    Gildow, F.E., Damsteegt, V.D., Stone, A.L., Schneider, W.L., Luster, D.G., Levy, L. 2004. Transmission of two north american isolates of plum pox virus: identification of aphid vectors and species-specific transmission from infected stone fruits. Acta Horticulture 657:207-212
    
  • TRANSMISSION OF THREE NORTH AMERICAN ISOLATES OF PLUM POX VIRUS: IDENTIFICATION OF APHID VECTORS AND SPECIES-SPECIFIC TRANSMISSION FROM INFECTED STONE FRUITS-(Proceedings)
    Gildow, F., Damsteegt, V.D., Stone, A.L., Schneider, W.L., Luster, D.G., Levy, L. 2004. Transmission of three north american isolates of plum pox virus: identification of aphid vectors and species-specific transmission from infected stone fruits. Acta Horticulture 657:207-212
    
  • POTENTIAL PRUNUS HOST RANGE OF PPV-PENN ISOLATES BY APHID TRANSMISSION-(Proceedings)
    Damsteegt, V.D., Stone, A.L., Gildow, F.E., Schneider, W.L., Luster, D.G. 2004. Potential prunus host range of ppv-penn isolates by aphid transmission. Acta Horticulture 657:201-206
    
  • DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS IN APHID VECTORS BY REAL-TIME FLUORESCENT REVERSE TRANSCRIPTION-PCR-(Peer Reviewed Journal)
    Acta Horticulture 657:135-140
    
  • PLUM POX IN NORTH AMERICA: IDENTIFICATION OF APHID VECTORS AND A ROLE FOR FRUIT IN VIRUS SPREAD-(Peer Reviewed Journal)
    Gildow, F., Damsteegt, V.D., Stone, A.L., Schneider, W.L., Luster, D.G., Levy, L. 2004. Plum pox in north america: identification of aphid vectors and a role for fruit in virus spread. Phytopathology. 94:868-874.
    
  • SPECIFIC DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS BY REAL-TIME FLUORESCENT REVERSE TRANSCRIPTION-PCR-(Peer Reviewed Journal)
    Schneider, W.L., Sherman, D.J., Stone, A.L., Damsteegt, V.D., Frederick, R.D. 2004. Specific detection and quantification of plum pox potyvirus by real-time fluorescent reverse transcription-PCR. Journal of Virological Methods.120:97-105.
    
  • COMPARATIVE HOST RANGE OF U.S. ISOLATES OF PLUM POX VIRUS AMONG PRUNUS AND OTHER WOODY PLANT SPECIES FOLLOWING GRAFT INOCULATION OR APHID TRANSMISSION-(Abstract Only)
    Damsteegt, V.D., Scorza, R., Gildow, F.E., Schneider, W.L., Stone, A.L., Luster, D.G. 2004. Comparative host range of U.S. isolates of plum pox virus among Prunus and other woody plant species following graft inoculation or aphid transmission. Phytopathology. 94:S24.
    
  • DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS DOUBLE-STRANDED RNA WITH REAL-TIME PCR-(Abstract Only)
    Schneider, W.L., Stone, A.L., Sherman, D.J., Damsteegt, V.D. 2004. Detection and quantification of plum pox potyvirus double-stranded RNA with real-time PCR. Phytopathology. 94:S93.
    
  • HOST ADAPTATION AND MEMORY IN PLUM POX POTYVIRUS POPULATIONS-(Abstract Only)
    Schneider, W.L., Stone, A.L., Wallis, C., Sherman, D.J., Shank, K., Damsteegt, V.D., Gildow, F. 2003. Host adaptation and memory in plum pox potyvirus populations. Phytopathology 93:S100.
    
  • POTENTIAL PRUNUS HOST RANGE OF PPV-PENN ISOLATES BY APHID TRANSMISSION-(Proceedings)
    Damsteegt, V.D., Stone, A.L., Gildow, F.E., Schneider, W.L., Luster, D.G. 2004. Potential prunus host range of ppv-penn isolates by aphid transmission. Acta Horticulture Proceedings. p. 46.
    
  • SPECIFIC DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS BY REAL-TIME FLUORESCENT RT-PCR-(Abstract Only)
    
    
  • APHID VECTOR COMPETENCE AND TRANSMISSION OF PENNSYLVANIA ISOLATES OF PLUM POX VIRUS FROM INFECTED PEACH FRUIT-(Abstract Only)
    GILDOW, F.E., DAMSTEEGT, V.D., STONE, A.L., LUSTER, D.G. APHID VECTOR COMPETENCE AND TRANSMISSION OF PENNSYLVANIA ISOLATES OF PLUM POX VIRUS FROM INFECTED PEACH FRUIT. AMERICAN PHYTOPATHOLOGICAL SOCIETY ABSTRACTS. 2002.
    
  • THE VERSATILITY OF PRUNUS TOMENTOSA AS A BIOINDICATOR OF VIRUSES-(Proceedings)
    
    
  • THE VERSATILITY OF PRUNUS TOMENTOSA AS A BIOINDICATOR OF VIRUSES-(Abstract Only)
    
    
  • SOYBEAN DWARF BEAN LEAF ROLL AND BEET WESTERN YELLOWS LUTEOVIRUSES IN SOUTHEASTERN U.S. WHITE CLOVER-(Peer Reviewed Journal)
    
    

Committee and Location Service

• FDWSRU Safety Committee, centrifuge safety officer, 2000-present
• FDWSRU Pesticide applicator 2013-present
• FDWSRU Essential personnel during Pandemic 2020-2023
• Member of the HazMat team of the Ft. Detrick Fire Dept. 1988-1989

Awards and Recognition

• APHIS Deputy Administrator’s Outstanding Achievement Award” Your contribution has been essential in eradication of PPV from PA. USDA, APHIS, PPQ would like to distinguish your hard work and wishes to recognize you as a key member of this significant achievement.” 2010
• US ARMY Certificate of Merit for Safety for “outstanding achievement for the prevention of accidents” 1996