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United States Department of Agriculture

Agricultural Research Service

Related Topics

Rong Wang
Meat Safety & Quality Research
Research Microbiologist
Rong.Wang@ars.usda.gov
Phone: (402) 762-4228
Fax: (402) 762-4149
P.O. BOX 166
CLAY CENTER, NE, 68933-0166

Projects
Genomic and Metagenomic Differences in Foodborne Pathogens and Determination of Ecological Niches and Reservoirs
Appropriated (D)
  Accession Number: 430418
Mitigation Approaches for Foodborne Pathogens in Cattle and Swine for Use During Production and Processing
Appropriated (D)
  Accession Number: 430703
Determination of Potential Contribution of Biofilm Formation and Sanitizer Resistance to Beef Product Contamination by Salmonella Enterica
Trust (T)
  Accession Number: 427965

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Characterization of enterohemorrhagic E. coli on veal hides and carcasses - (Peer Reviewed Journal) - (18-Aug-16)
Escherichia coli O157:H7 strains isolated from “High Event Period” beef contamination have strong biofilm forming ability and low sanitizer susceptibility which are associated with high pO157 plasmid copy number - (Peer Reviewed Journal) - (13-Jun-16)
Complete and closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Anatum isolated from human and bovine sources Reprint Icon - (Peer Reviewed Journal)
Nguyen, S.V., Harhay, D.M., Bono, J.L., Smith, T.P.L., Fields, P.I., Dinsmore, B.A., Santovenia, M., Kelley, C.M., Wang, R., Bosilevac, J.M., Harhay, G.P. 2016. Complete and closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Anatum isolated from human and bovine sources. Genome Announcements. Vol. 4(3): e00447-16. doi: 10.1128/genomeA.00447-16.
Contamination revealed by indicator microorganism levels during veal processing - (Peer Reviewed Journal)
Bosilevac, J.M., Wang, R., Luedtke, B.E., Wheeler, T.L., Koohmaraie, M. 2016. Contamination revealed by indicator microorganism levels during veal processing. Journal of Food Protection. 79(8):1341-1347. doi:10.4315/0362-028X.JFP-15-572
Evaluation of Salmonella biofilm cell transfer from contact surfaces to beef products - (Abstract Only) - (15-Mar-16)
Development and application of a green fluorescent protein (GFP) expressing E. coli O103 surrogate for tracking contamination through grinding and identifying persistent points of contamination - (Abstract Only)
Bosilevac, J.M., Luedtke, B.E., Wang, R., Ogunrinola, Y. 2016. Development and application of a green fluorescent protein (GFP) expressing E. coli O103 surrogate for tracking contamination through grinding and identifying persistent points of contamination.[abstract] Beef Industry Safety Summit, March 1-3, 2016, Austin, Texas. Available: http://www.bifsco.org/2016researchsessionabstracts.aspx.
Mobilome differences between Salmonella enterica serovars Anatum and Typhimurium isolated from cattle and humans and potential impact on virulence - (Abstract Only)
Nguyen, S.V., Bono, J.L., Smith, T.P.L., Kelley, C.M., Wang, R., Harhay, G.P., Harhay, D.M. 2016. Mobilome differences between Salmonella enterica serovars Anatum and Typhimurium isolated from cattle and humans and potential impact on virulence. [Abstract] In proceedings: 60th Wind River Conference on Procaryotic Biology, 2016 June 1-5. Estes Park, Colorado. p. 67.
Diagnostic accuracy of rectoanal mucosal swab of feedlot cattle for detection and enumeration of Salmonella enterica Reprint Icon - (Peer Reviewed Journal)
Agga, G.E., Arthur, T.M., Schmidt, J.W., Wang, R., Harhay, D.M. 2016. Diagnostic accuracy of rectoanal mucosal swab of feedlot cattle for detection and enumeration of Salmonella enterica. Journal of Food Protection. 79(4):531-537. doi:10.4315/0362-028X.JFP-15-409.
Complete closed genome sequences of Salmonella enterica subsp. enterica serotypes Anatum, Montevideo, Typhimurium and Newport, isolated from beef, cattle, and humans Reprint Icon - (Peer Reviewed Journal)
Harhay, D.M., Bono, J.L., Smith, T.P., Fields, P.I., Dinsmore, B.A., Santovenia, M., Kelley, C.M., Wang, R., Harhay, G.P. 2016. Complete closed genome sequences of Salmonella enterica subsp. enterica serotypes Anatum, Montevideo, Typhimurium and Newport, isolated from beef, cattle, and humans. Genome Announcements. 4(1):e01683-15. doi:10.1128/genomeA.01683-15.
Sequence of colonization determines the composition of mixed biofilms by Escherichia coli O157:H7 and O111:H8 strains Reprint Icon - (Peer Reviewed Journal)
Wang, R., Kalchayanand, N., Bono, J.L. 2015. Sequence of colonization determines the composition of mixed biofilms by Escherichia coli O157:H7 and O111:H8 strains. Journal of Food Protection. 78(8):1554-1559. DOI: 10.4315/0362-028X.JFP-15-009.
Comparative analysis of twenty-four complete aenome aequences of Salmonella enterica Serotypes Anatum, Montevideo, Typhimurium and Newport isolated from ground beef or asymptomatic cattle on farm or at harvest - (Abstract Only)
Harhay, D.M., Bono, J.L., Smith, T.P., Kalbfleisch, T., Kelley, C., Wang, R., Harhay, G.P. 2015. Comparative analysis of twenty-four complete aenome aequences of Salmonella enterica Serotypes Anatum, Montevideo, Typhimurium and Newport isolated from ground beef or asymptomatic cattle on farm or at harvest [abstract]. American Society for Microbiology 115th General Meeting. Poster No. 397, Session 029.
Characterization of E. coli O157:H7 strains isolated from “High Event Period” beef trim contamination - (Abstract Only)
Wang, R., Luedtke, B.E., Kalchayanand, N., Schmidt, J.W., Bosilevac, J.M., Arthur, T.M. 2015. Characterization of E. coli O157:H7 strains isolated from “High Event Period” beef trim contamination. [Abstract] International Association for Food Protection Proceedings. Volume 78 (Supplement A): page 64. (T10-211).
Genomic comparison of Salmonella enterica serotypes commonly associated with cattle and beef – Analysis of variation in virulence potential - (Abstract Only)
Harhay, D.M., Bono, J.L., Wang, R., Smith, T.P., Harhay, G.P., Kalbfleisch, T., Wheeler, T.L. 2015. Genomic comparison of Salmonella enterica serotypes commonly associated with cattle and beef – Analysis of variation in virulence potential. [Abstract]. Beef Industry Safety Summit. http://www.bifsco.org/2015researchsessionabstracts.aspx.
Efficacy of antimicrobial compounds on surface decontamination of seven shiga toxin-producing Escherichia coli (STEC) and Salmonella inoculated onto fresh beef Reprint Icon - (Peer Reviewed Journal)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L. 2015. Efficacy of antimicrobial compounds on surface decontamination of seven shiga toxin-producing Escherichia coli and Salmonella inoculated onto fresh beef. Journal of Food Protection. 78(3):503-510.
Occurrence of antimicrobial-resistant Escherichia coli and Salmonella enterica in the beef cattle production and processing continuum Reprint Icon - (Peer Reviewed Journal)
Schmidt, J.W., Agga, G.E., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wang, R., Wheeler, T.L., Arthur, T.M. 2015. Occurrence of antimicrobial-resistant Escherichia coli and Salmonella enterica in the beef cattle production and processing continuum. Applied and Environmental Microbiology. 81(2):713-725.
Biofilm formation and sanitizer resistance of Escherichia coli 0157:H7 strains isolated from "High Event Period" meat contamination Reprint Icon - (Peer Reviewed Journal)
Wang, R., Kalchayanand, N., King, D.A., Luedtke, B.E., Bosilevac, J.M., Arthur, T.M. 2014. Biofilm formation and sanitizer resistance of Escherichia coli 0157:H7 strains isolated from "High Event Period" meat contamination. Journal of Food Protection. 77(11):1982-1987.
Effect of direct-fed microbial supplementation on the presence of Salmonella enterica in bovine peripheral lymph nodes - (Abstract Only)
Harhay, D.M., Loneragan, G.H., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Wheeler, T.L., Kolath, B., Theunick, D., Riemann, M., Schaefer, D., Brown, T. 2014. Effect of direct-fed microbial supplementation on the presence of Salmonella enterica in bovine peripheral lymph nodes. International Association for Food Protection. Supp. A(77):211-212. P3-65.
Effect of residual sanitizers on Salmonella enterica biofilm formation - (Abstract Only)
Wang, R., Harhay, D.M. 2014. Effect of residual sanitizers on Salmonella enterica biofilm formation. International Association for Food Protection. Supp. A(77):185. P2-163.
Examination of predictors of Salmonella enterica contamination in cattle feedlot environments - (Abstract Only)
Harhay, D.M., Arthur, T.M., Schmidt, J.W., Wang, R., Kalchayanand, N., Bosilevac, J.M., Edrington, T.S., Loneragan, G., Wheeler, T.L. 2014. Examination of predictors of Salmonella enterica contamination in cattle feedlot environments. [Abstract] Beef Industray Safety Summit. Available: www.bifsco.org/attendees.aspx.
Minimal heat treatments reduce Shiga toxin-producing Escherichia coli (STEC)and Salmonella on the surface of fresh beef - (Abstract Only)
Kalchayanand, N., Wang, R., Wheeler, T.L. 2014. Minimal heat treatments reduce Shiga toxin-producing Escherichia coli (STEC)and Salmonella on the surface of fresh beef.[Abstract] Annual Meeting of the Institute of Food Technologists. Available:http://www.ift.org/Meetings-and-Events/Past-Meeting-Resources/Technical-Abstract-Search.
Effects of in-plant interventions on reduction of enterohemorrhagic Escherichia coli and background indicator microorganisms on veal calf hides Reprint Icon - (Peer Reviewed Journal)
Wang, R., Koohmaraie, M., Wheeler, T.L., Bosilevac, J.M. 2014. Effects of in-plant interventions on reduction of enterohemorrhagic Escherichia coli and background indicator microorganisms on veal calf hides. Journal of Food Protection. 77(5):745-751.
Immersion in antimicrobial solutions reduces Salmonella enterica and Shiga toxin-producing Escherichia coli on beef cheek meat Reprint Icon - (Peer Reviewed Journal)
Schmidt, J.W., Bosilevac, J.M., Kalchayanand, N., Wang, R., Wheeler, T.L., Koohmaraie, M. 2014. Immersion in antimicrobial solutions reduces Salmonella enterica and Shiga toxin-producing Escherichia coli on beef cheek meat. Journal of Food Protection. 77(4):538-548.
Impact of sampling area and location on measurement of indicator organisms during beef carcass interventions Reprint Icon - (Peer Reviewed Journal)
Wang, R., King, D.A., Koohmaraie, M., Bosilevac, J.M. 2013. Impact of sampling area and location on measurement of indicator organisms during beef carcass interventions. Journal of Food Protection. 76(12):2069-2073.
Mixed biofilm formation by Shiga toxin-producing Escherichia coli and Salmonella enterica serovar typhimurium enhanced bacterial resistance to sanitization due to extracellular polymeric substances Reprint Icon - (Peer Reviewed Journal)
Wang, R., Kalchayanand, N., Schmidt, J.W., Harhay, D.M. 2013. Mixed biofilm formation by Shiga toxin-producing Escherichia coli and Salmonella enterica serovar typhimurium enhanced bacterial resistance to sanitization due to extracellular polymeric substances. Journal of Food Protection. 76(9):1513-1522.
Biofilm formation and sanitizer resistance contributes to “High Event” meat contamination by E. coli O157:H7 - (Abstract Only)
Wang, R., Kalchayanand, N., Bosilevac, J.M., Arthur, T.M. 2013. Biofilm formation and sanitizer resistance contributes to “High Event” meat contamination by E. coli O157:H7. International Association for Food Protection Proceedings. [abstract] P1-48 Page No.48.
Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest Reprint Icon - (Peer Reviewed Journal)
Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Brichta-Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10:368-374.
Salmonella in lymph nodes of cull and fed cattle at harvest - (Abstract Only)
Webb, H.E., Harhay, D.M., Gragg, S.E., Brashears, M.M., Nightingale, K.K., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Lonergan, G.H. 2013. Salmonella in lymph nodes of cull and fed cattle at harvest. IN: Proceedings of The Beef Industry Summit 2013, March 13-15, 2013, Dallas Texas, 2013 FLASHDRIVE.
Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest Reprint Icon - (Peer Reviewed Journal)
Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Edrington, T.S., Harhay, D.M. 2013. Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest. Foodborne Pathogens and Disease. 10(4):368-374.
The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests Reprint Icon - (Peer Reviewed Journal)
Wang, R., Schmidt, J.W., Arthur, T.M., Bosilevac, J.M. 2013. The physiologic state of Escherichia coli O157:H7 does not affect its detection in two commercial real-time PCR-based tests. Food Microbiology. 33: 205-212.
Dual-serotype biofilm formation by Shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains - (Peer Reviewed Journal)
SWang, R., Kalchayanand, N., Schmidt, J.W., Bosilevac, J.M., Bono, J.L. 2012. Dual-serotype biofilm formation by Shiga toxin-producing Escherichia coli 0157:H7 and 026:H11 strains. Applied and Environmental Microbiology. 78(17):6341-6344.
Biofilm formation by Shiga toxin–producing Escherichia coli O157:H7 and non-O157 strains and their tolerance to sanitizers commonly used in the food processing environment Reprint Icon - (Peer Reviewed Journal)
Wang, R., Bono, J.L., Kalchayanand, N., Shackelford, S.D., Harhay, D.M. 2012. Biofilm formation by Shiga toxin–producing Escherichia coli O157:H7 and non-O157 strains and their tolerance to sanitizers commonly used in the food processing environment. Journal of Food Protection. 75(8):1418-1428.
Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7 - (Peer Reviewed Journal)
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L. 2012. Evaluation of commonly used antimicrobial interventions for fresh beef inoculated with Shiga toxin-producing Escherichia coli serotypes O26, O45, O103, O111, O121, O145, and O157:H7. Journal of Food Protection. 75(7):1207-1212.
Biofilm formation by foodborne pathogens and their resistance to sanitization - (Abstract Only)
Wang, R., Bono, J.L., Kalchayanand, N., Shackelford, S.D., Harhay, D.M. 2012. Biofilm formation by foodborne pathogens and their resistance to sanitization. [Abstract]. Americian Society for Microbiology, 112th General Meeting. Poster No 2154, session 170.
Efficacy of hypobromous acid as a hide-on carcass antimicrobial intervention Reprint Icon - (Peer Reviewed Journal)
Schmidt, J.W., Wang, R., Kalchayanand, N., Wheeler, T.L., Koohmaraie, M. 2012. Efficacy of hypobromous acid as a hide-on carcass antimicrobial intervention. Journal of Food Protection. 75 (5): 955-958.
Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica - (Peer Reviewed Journal)
Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Loneragan, G.H., Wheeler, T.L. 2012. Microbiological analysis of bovine lymph nodes for the detection of Salmonella enterica. Journal of Food Protection. 75(5):854-858.
Salmonella in lymph nodes of cattle presented for harvest - (Abstract Only)
Gragg, S.E., Loneragan, G.H., Brashears, M.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brichta-Harhay, D.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. Proceeding of the 2011 Conference of Research Workers in Animal Diseases, December 4-6, 2011, Chicago, Illinois. p. 149.
Salmonella in lymph nodes of cattle presented for harvest - (Abstract Only)
Gragg, S.E., Loneragan, G.H., Brichta-Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wang, R., Schmidt, J.W., Brooks, J.C., Shackelford, S.D., Wheeler, T.L., Brown, T.R., Brashears, M.M. 2011. Salmonella in lymph nodes of cattle presented for harvest [abstract]. International Association for Food Protection. p. 19. Abstract No. P1-62.
Towards an understanding of how Salmonella potentially evades beef carcass processing interventions - (Abstract Only)
Brichta-Harhay, D.M., Gragg, S.E., Smith, T.P.L., Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Schmidt, J.W., Wang, R., Shackelford, S.D., Wheeler, T.L., Brashears, M.M., Brooks, J.C., Brown, T.R., Harhay, G.P., Loneragan, G.H. 2011. Towards an understanding of how Salmonella potentially evades beef carcass processing interventions [abstract]. 2011 Beef Industry Safety Summit. Abstract No. 2.
Last Modified: 8/24/2016