Projects

PREVENTION AND CHARACTERIZATION OF PERSISTENT COLONIZATION BY E. COLI O157:H7 AND OTHER SHIGA TOXIN-PRODUCING E. COLI (STEC) IN CATTLE Appropriated (D)   Accession Number: 420815

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Evaluation of the impact of quorum sensing transcriptional regulator SdiA on long-term persistence and fecal shedding of Escherichia coli O157:H7 in weaned calves - (Peer Reviewed Journal)

Sharma, V.K., Bearson, S.M. 2013. Evaluation of the impact of quorum sensing transcriptional regulator SdiA on long-term persistence and fecal shedding of Escherichia coli O157:H7 in weaned calves. Microbial Pathogenesis. 57:21-26.

Hha controls Escherichia coli O157:H7 biofilm formation by differential regulation of global transcriptional regulators FlhDC and CsgD - (Peer Reviewed Journal)

Sharma, V.K., Bearson, B.L. 2013. Hha controls Escherichia coli O157:H7 biofilm formation by differential regulation of global transcriptional regulators FlhDC and CsgD. Applied and Environmental Microbiology. 79(7):2384.

Quorum sensing transcriptional regulator QseA is essential for the expression of multiple virulence regulons of enterohemorrhagic Escherichia coli O157:H7 - (Abstract Only)

Sharma, V.K. 2012. Quorum sensing transcriptional regulator QseA is essential for the expression of multiple virulence regulons of enterohemorrhagic Escherichia coli O157:H7 [abstract]. In: Proceedings of the 8th International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia Coli. May 5-9, 2012, Amsterdam, The Netherlands. 59:8.

Quorum sensing transcriptional regulator QseA is essential for the expression of multiple virulence regulons of enterohemorrhagic Escherichia coli O157:H7 - (Proceedings)

Sharma, V.K. 2012. Quorum sensing transcriptional regulator QseA is essential for the expression of multiple virulence regulons of enterohemorrhagic Escherichia coli O157:H7. Zoonoses and Public Health. 59:(Suppl. 1), 2-18.

Correlating levels of type III secretion and secreted proteins with fecal shedding of Escherichia coli O157:H7 in cattle - (Peer Reviewed Journal)

Sharma, V.K., Sacco, R.E., Kunkle, R.A., Bearson, S.M., Palmquist, D.E. 2012. Correlating levels of type III secretion and secreted proteins with fecal shedding of Escherichia coli O157:H7 in cattle. Infection and Immunity. 80(4):1333-1342.

Role of curli and cellulose expression by Escherichia coli O157:H7 on the cell’s ability to attach to spinach - (Peer Reviewed Journal)

Macarisin, D., Patel, J.R., Bauchan, G.R., Giron, J., Sharma, V.K. 2012. Role of curli and cellulose expression by Escherichia coli O157:H7 on the cell’s ability to attach to spinach. Foodborne Pathogens and Disease. 9(2):160-167.

Enterohemorrhagic Escherichia coli O157:H7 requires quorum sensing transcriptional regulators QseA and SdiA for colonization and persistence in the bovine intestinal tract - (Abstract Only)

Sharma, V.K., Bearson, S.M., Thacker, T.C. 2011. Enterohemorrhagic Escherichia coli O157:H7 requires quorum sensing transcriptional regulators QseA and SdiA for colonization and persistence in the bovine intestinal tract [abstract]. 71st Annual Meeting of the North Central Branch of the American Society for Microbiology. October 7-8, 2011, Des Moines, Iowa. 59:147.

Evaluation of Hha and Hha SepB Mutant Strains of Escherichia coli O157:H7 as Bacterins for Reducing E. coli O157:H7 Shedding in Cattle - (Peer Reviewed Journal)

Sharma, V.K., Nystrom, E.A., Casey, T. 2011. Evaluation of hha and hha sepB mutant strains of Escherichia coli O157:H7 as bacterins for reducing E. coli O157:H7 shedding in cattle. Vaccine. 29(31):5078-5086. Available: http://www.sciencedirect.com/science/article/pii/S0264410X11006189.

A Mutation in the PoxA Gene of Salmonella enterica Serovar Typhimurium Results in Altered Protein Production, Elevated Susceptibility to Environmental Challenges, and Decreased Swine Colonization - (Peer Reviewed Journal)

Bearson, S.M., Bearson, B.L., Brunelle, B.W., Sharma, V.K., Lee, I. 2011. A mutation in the poxA gene of Salmonella enterica serovar Typhimurium results in altered protein production, elevated susceptibility to environmental challenges, and decreased swine colonization. Foodborne Pathogens and Disease. 8(6):725-732.

Regulation of Biofilm Formation in Escherichia coli O157:H7 - (Abstract Only)

Sharma, V.K. Regulation of Biofilm Formation in Escherichia coli O157:H7 [abstract]. 45th United States-Japan Cooperative Program in Natural Resources Panel of Animal and Avian Health Meeting. p. 16.

Comprehensive analysis of CLE polypeptide signaling gene expression and overexpression activity in Arabidopsis - (Peer Reviewed Journal)

Jun, J., Fiume, E., Roeder, A.H., Meng, L., Sharma, V.K., Osmont, K.S., Baker, C., Ha, C., Meyerowitz, E.M., Feldman, L.J., Fletcher, J.C. 2010. Comprehensive analysis of CLE polypeptide signaling gene expression and overexpression activity in Arabidopsis. Plant Physiology. 154:1721-1736.

Hha Represses Biofilm Formation in Escherichia coli O157:H7 by Affecting the Expression of Flagella and Curli Fimbriae - (Abstract Only)

Sharma, V.K., Bearson, B.L., Casey, T. 2010. Hha Represses Biofilm Formation in Escherichia coli O157:H7 by Affecting the Expression of Flagella and Curli Fimbriae[abstract]. American Society for Microbiology Meeting. p350.

Evaluation of the Effects of SDIA, a LUXR Homologue, on Adherence and Motility of Escherichia coli O157:H7 - (Peer Reviewed Journal)

Sharma, V.K., Bearson, S.M., Bearson, B.L. 2010. Evaluation of the Effects of SDIA, a LUXR Homologue, on Adherence and Motility of Escherichia coli O157:H7. Microbiology. 156(5):1303-1312.

Yeast Cell-Wall Products Containing Beta-Glucan Plus Ascorbic Acid Affect Neonatal Bos-Taurus Calf Leukocytes and Growth after a Transport Stressor - (Peer Reviewed Journal)

Eicher, S.D., Wesley, I.V., Sharma, V.K., Johnson, T.R. 2010. Yeast Cell-Wall Products Containing Beta-Glucan plus Ascorbic Acid affect Neonatal Bos-Taurus Calf Leukocytes and Growth after a Transport Stressor. Journal of Animal Science. 88(3):1195-1203.

EVALUATING THE ROLE OF SDIA AND HHA IN ENHANCED ADHERENCE OF A SDIA HHA DOUBLE MUTANT OF ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 - (Abstract Only)

Sharma, V.K., Bearson, S.M., Bearson, B.L. 2009. Evaluating the Role of SDIA and HHA in Enhanced Adherence of a SDIA HHA Double Mutant of Enterohemorrhagic Escherichia coli O157:H7 [abstract]. American Society for Microbiology Meeting. Paper No. B108.

EFFECTS OF SHIGA TOXIN ON ISOLATED PORCINE GRANULOCYTES - (Abstract Only) - (18-Jul-08)

CHARACTERIZATION OF A 3.3-KB PLASMID OF ESCHERICHIA COLI O157:H7 AND EVALUATION OF STABILITY OF GENETICALLY ENGINEERED DERIVATIVES OF THIS PLASMID EXPRESSING GREEN FLUORESCENCE - (Peer Reviewed Journal)

Sharma, V.K., Stanton, T.B. 2008. Characterization of a 3.3-kb plasmid of Escherichia coli O157:H7 and evaluation of stability of genetically engineered derivatives of this plasmid expressing green fluorescence. Veterinary Microbiology. Available: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TD6-4SKB3CT-2&_user=6956098&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000052423&_version=1&_urlVersion=0&_userid=6956098&md5=4acc7d9e43e9b59307dc8e59dc8022c6.

Evaluation of the Control of Pathogen Load by an Anti-Salmonella Bacterium in a Herd of Cattle with Persistent Salmonella Infection - (Peer Reviewed Journal)

Patton, T.G., Sharma, V.K., Carlson, S.A. 2009. Evaluation of the Control of Pathogen Load by an Anti-Salmonella Bacterium in a Herd of Cattle with Persistent Salmonella Infection. American Journal of Veterinary Research. 70(1):92-98.

COLLATERAL EFFECTS OF ANTIBIOTICS - CARBADOX AND METRONIDAZOLE INDUCE VSH-1 AND FACILITATE GENE TRANSFER AMONG BRACHYSPIRA HYODYSENTERIAE STRAINS - (Peer Reviewed Journal)

Stanton, T.B., Humphrey, S.B., Sharma, V.K., Zuerner, R.L. 2008. Collateral effects of antibiotics - carbadox and metronidazole induce VSH-1 and facilitate gene transfer among Brachyspira hyodysenteriae strains. Applied and Environmental Microbiology. 74(10):2950-2956.

ISOLATION OF AN ANTI-SALMONELLA BACTERIUM THAT MAY CONTROL PATHOGEN LOAD IN PERSISTENTLY-INFECTED CATTLE HERDS - (Abstract Only) - (21-Feb-08)

EVALUATION OF THE EFFECTS OF QUORUM-SENSING SIGNALING PATHWAYS ON THE EXPRESSION OF HHA AND OTHER REGULATORS OF LEE EXPRESSION IN ESCHERICHIA COLI O157:H7 - (Abstract Only)

Sharma, V.K., Bearson, S.M., Bearson, B.L. 2008. Evaluation of the effects of quorum-sensing signaling pathways on the expression of hha and other regulators of LEE expression in Escherichia coli O157:H7 [abstract]. American Society for Microbiology Meeting. Paper No. H036.

CHARACTERIZATION OF A CRYPTIC PLASMID FROM ESCHERICHIA COLI O157:H7 AND EVALUATION OF THE EXPRESSION OF GREEN-FLUORESCENT PROTEIN FROM GENETICALLY ENGINEERED DERIVATIVES OF THIS PLASMID - (Abstract Only)

Sharma, V.K., Stanton, T.B. 2007. Characterization of a cryptic plasmid from Escherichia coli O157:H7 and evaluation of the expression of green-fluorescent protein from genetically engineered derivatives of this plasmid [abstract]. 67th North Central Branch, American Society for Microbiology Meeting. p. 5.

EXPERIMENTAL REPRODUCTION OF BOVINE SALMONELLA ENCEPHALOPATHY USING A NOREPINEPHRINE-BASED STRESS MODEL - (Peer Reviewed Journal)

McCuddin, Z.P., Carlson, S.A., Sharma, V.K. 2007. Experimental reproduction of bovine Salmonella encephalopathy using a norepinephrine-based stress model. British Veterinary Journal. Available:http://www.sciencedirect.com

INVOLVEMENT OF A SALMONELLA GENOMIC ISLAND 1 GENE IN THE RUMEN PROTOZOAN-MEDIATED ENHANCEMENT OF INVASION FOR MULTIPLE-ANTIBIOTIC-RESISTANT SALMONELLA ENTERICA SEROVAR TYPHIMURIUM - (Peer Reviewed Journal)

Carlson, S.A., Sharma, V.K., McCuddin, Z.P., Rasmussen, M.A., Franklin, S.L. 2007. Involvement of a Salmonella genomic island 1 gene in the rumen protozoan-mediated enhancement of invasion for multiple-antibiotic-resistant Salmonella enterica Serovar Typhimurium. Infection and Immunity. 75(2):792-800.

SHIGA TOXIN AND THE SHIGA TOXIN-ENCODING PHAGE DO NOT FACILITATE E. COLI O157:H7 COLONIZATION IN SHEEP - (Peer Reviewed Journal)

Cornick, N.A., Helgerson, A.F., Sharma, V.K. 2007. Shiga toxin and shiga toxin-encoding phage do not facilitate E. coli O157:H7 colonization in sheep. Applied and Environmental Microbiology. 73(1):344-346.

EDEMA DISEASE CAUSED BY A CLONE OF ESCHERICHIA COLI O147 - (Peer Reviewed Journal)

Helgerson, A.F., Sharma, V.K., Dow, A.M., Schroeder, R., Post, K., Cornick, N.A. 2006. Edema disease caused by a clone of Escherichia coli O147. Journal of Clinical Microbiology. 44(9):3074-7.

REAL-TIME REVERSE TRANSCRIPTION-MULTIPLEX PCR FOR SIMULTANEOUS AND SPECIFIC DETECTION OF RFBE AND EAE GENES OF ESCHERICHIA COLI O157:H7 - (Peer Reviewed Journal)

Sharma, V.K. 2006. Real-time reverse transcription-multiplex PCR for simultaneous and specific detection of rfbE and eae genes of Escherichia coli O157:H7. Molecular and Cellular Probes. 20(5):298-306.

IDENTIFICATION OF HHA AS A POTENTIAL SUBSTRATE FOR THE CLPXP PROTEASE, A MODULATOR OF LEE EXPRESSION IN ENTEROHEMORRHAGIC ESCHERICHIA COLI 0157:H7 - (Abstract Only)

Mesterhazy, K.A., Sharma, V.K. 2006. Identification of Hha as a potential substrate for the ClpXP protease, a modulator of LEE expression in enterohemorrhagic Escherichia coli 0157:h7 [abstract]. American Society for Microbiology. p.264.

ANTIMICROBIAL COLLATERAL EFFECTS: INDUCTION OF THE PROPHAGE-LIKE, GENE TRANSFER AGENT VSH-1 IN CULTURES OF THE SWINE PATHOGEN BRACHYPSIRA HYODYSENTERIAE - (Abstract Only)

Stanton, T.B., Sharma, V.K., Humphrey, S.B., Zuerner, R.L. 2006. Antimicrobial collateral effects: Induction of the prophage-like, gene transfer agent VSH-1 in cultures of the swine pathogen Brachypsira hyodysenteriae [abstract]. American Society for Microbiology. May 20-26, 2006, Orlando, Florida. 2006 CDROM.

DIFFERENTIAL REGULATION OF SEPL AND ESP GENES OF ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 - (Abstract Only)

Sharma, V.K., Casey, T., Morgan, R.W. 2005. Differential regulation of sepL and esp genes of enterohemorrhagic Escherichia coli O157:H7 [abstract]. Joint Meeting of the 3 Divisions of the International Union of Microbiological Societies. p. 159.

EXPOSURE TO RUMEN PROTOZOA LEADS TO ENHANCEMENT OF PATHOGENICITY OF AND INVASION BY MULTIPLE-ANTIBIOTIC-RESISTANT SALMONELLA ENTERICA BEARING SGI1 - (Peer Reviewed Journal)

Rasmussen, M.A., Carlson, S.A., Franklin, S.L., McCuddin, Z.P., Wu, M.T., Sharma, V.K. 2005. Exposure to rumen protozoa leads to enhancement of pathogenicity of and invasion by multiple-antibiotic-resistant Salmonella enterica bearing SGI1. Infection and Immunity. 73(8):4668-4675.

VSH-1, A PROPHAGE-LIKE, GENE TRANSFER AGENT OF BRACHYSPIRA HYODYSENTERIAE AND (PROBABLY) OF OTHER BRACHYSPIRA SPECIES - (Abstract Only)

Stanton, T.B., Matson, E.G., Humphrey, S.B., Zuerner, R.L., Sharma, V.K. 2005. VSH-1, a prophage-like, gene transfer agent of Brachyspira hyodysenteriae and (probably) of other Brachyspira species [abstract]. Third International Conference on Colonic Spirochaetal Infections in Animals and Humans. p. 15-16.

RAPID PCR DETECTION OF ENTEROHEMORRHAGIC ESCHERICHIA COLI (EHEC) IN BOVINE FOOD PRODUCTS AND FECES - (Peer Reviewed Journal)

Ellingson, J.L.E., Koziczkowski, J.J., Anderson, J.L., Carlson, S.A., Sharma, V.K. 2005. Rapid PCR detection of enterohemorrhagic Escherichia coli (EHEC) in bovine food products and feces. Molecular and Cellular Probes. 19(3):213-217.

HYPERADHERENCE OF AN HHA MUTANT OF ESCHERICHIA COLI O157:H7 IS CORRELATED WITH ENHANCED EXPRESSION OF LEE-ENCODED ADHERENCE GENES - (Peer Reviewed Journal)

Sharma, V.K., Carlson, S.A., Casey, T. 2005. Hyperadherence of an hha mutant of Escherichia coli O157:H7 is correlated with enhanced expression of LEE-encoded adherence genes. Federation of European Microbiological Societies Microbiology Letters. 243(1):189-196.

ROLE OF HHA AND LER IN TRANSCRIPTIONAL REGULATION OF THE ESP OPERON OF ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 - (Peer Reviewed Journal)

Sharma, V.K., Zuerner, R.L. 2004. Role of hha and ler in transcriptional regulation of the esp operon of enterohemorrhagic Escherichia coli O157:H7. Journal of Bacteriology. 186(21):7290-7301.

GENETIC RELATEDNESS OF ESCHERICHIA COLI O147 STRAINS CAUSING EDEMA DISEASE - (Abstract Only)

Helgerson, A.F., Schroeder, R., Sharma, V.K., Post, K., Cornick, N.A. 2004. Genetic relatedness of Escherichia coli O147 strains causing edema disease [abstract]. American Society for Microbiology. p. 671.

TWELVE HOUR REAL-TIME PCR TECHNIQUE FOR THE SENSITIVE AND SPECIFIC DETECTION OF SALMONELLA IN RAW AND READY-TO-EAT MEAT PRODUCTS - (Peer Reviewed Journal)

ELLINGSON, J.L., ANDERSON, J.L., CARLSON, S.A., SHARMA, V.K. TWELVE HOUR REAL-TIME PCR TECHNIQUE FOR THE SENSITIVE AND SPECIFIC DETECTION OF SALMONELLA IN RAW AND READY-TO-EAT MEAT PRODUCTS. MOLECULAR AND CELLULAR PROBES. 2004. V. 18. P. 51-57.

ESCHERICHIA COLI O147: AN EMERGING SEROGROUP OF EDEMA DISEASE OUTBREAKS - (Abstract Only)

HELGERSON, A., SCHROEDER, R., SHARMA, V.K., POST, K., CORNICK, N.A. ESCHERICHIA COLI O147: AN EMERGING SEROGROUP OF EDEMA DISEASE OUTBREAKS. CONFERENCE ON GASTROINTESTINAL FUNCTION. 2003. V. 26. P. 21.

IDENTIFICATION AND EVALUATION OF REGULATORY FACTORS MODULATING THE EXPRESSION OF LER IN ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 - (Abstract Only)

FENTON, K., CORNICK, N.A., MORGAN, R.W., SHARMA, V.K. IDENTIFICATION AND EVALUATION OF REGULATORY FACTORS MODULATING THE EXPRESSION OF LER IN ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7. AMERICAN SOCIETY FOR MICROBIOLOGY. 2003. ABSTRACT P. 96.

DETECTION OF ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 BY USING A MULTIPLEX REAL-TIME PCR ASSAY FOR GENES ENCODING INTIMIN AND SHIGA TOXINS - (Peer Reviewed Journal)

SHARMA, V.K., NYSTROM, E.A. DETECTION OF ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 BY USING A MULTIPLEX REAL-TIME PCR ASSAY FOR GENES ENCODING INTIMIN AND SHIGA TOXINS. VETERINARY MICROBIOLOGY. 2003. V. 93. P. 247-260.

GENETIC DIVERSITY OF ESCHERICHIA COLI O147 STRAINS IMPLICATED IN EDEMA DISEASE - (Abstract Only) - (13-Nov-02)

MULTIPLEX FLUOROGENIC REAL-TIME (TAQMAN) PCR FOR DETECTION AND QUANTIFICATION OF ESCHERICHIA COLI O157:H7 IN DAIRY WASTEWATER WETLANDS - (Peer Reviewed Journal) - (17-Jul-02)

ROLE OF SHIGA TOXIN IN THE ECOLOGY OF ESCHERICHIA COLI O157:H7 (3RD JOINT RRI-INRA GASTROINTESTINAL TRACT MICROBIOLOGY SYMPOSIUM, JUNE 12-15, 2002, ABERDEEN, SCOTLAND, UK) - (Abstract Only) - (15-Jun-02)

DETECTION AND QUANTITATION OF ENTEROHEMORRHAGIC ESCHERICHIA COLI O157, O111, AND 026 IN BEEF AND BOVINE FECES BY REAL-TIME POLYMERASE CHAIN REACTION - (Peer Reviewed Journal) - (19-Apr-02)

TRANSCRIPTIONAL REGULATION OF ESP GENES OF ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 - (Abstract Only) - (22-Feb-02)

TRANSCRIPTION OF THE GENE MEDIATING METHICILLIN RESISTANCE IN STAPHYLOCOCCUS AUREUS (MECA) IS CO-REPRESSED BUT NOT CO-INDUCED BY COGNATE MECA AND BETA-LACTAMASE REGULATORS - (Peer Reviewed Journal) - (14-Sep-01)

DEVELOPMENT AND APPLICATION OF A REAL-TIME PCR TO DETECT AND QUANTIFY ENTEROHEMORRHAGIC ESCHERICHIA COLI O157:H7 AND OTHER SHIGA TOXIN-PRODUCING E. COLI - (Abstract Only) - (24-May-01)

APPLICATION OF A MULTIPLEX PCR ASSAY FOR SIMULTANEOUS DETECTION OF SHIGA TOXIGENIC ESCHERICHIA COLI AND ENTEROHEMORRHAGIC E. COLI OF SEROTYPES O157:H7, O26, AND O111 - (Abstract Only) - (02-Nov-00)

SIMULTANEOUS DETECTION OF SALMONELLA AND ESCHERICHIA COLI O157:H7 USING FLUOROGENIC PCR AND SINGLE ENRICHMENT BROTH - (Peer Reviewed Journal) - (11-Sep-00)

SIMULTANEOUS IDENTIFICATION OF SALMONELLA AND ENTEROHEMORRHAGIC ESCHERICHIACOLI (EHEC) O157:H7 IN FECES AND FOODS USING AN ENRICHMENT BROTH CULTURE- FLUOROGENIC MULTIPLEX PCR ASSAY - (Abstract Only) - (25-May-00)

DEVELOPMENT OF FLUOROGENIC PCR ASSAYS FOR RAPID DETECTION OF VIRULENCE GENES OF ESCHERICHIA COLI O157:H7 AND OTHER SHIGA TOXIGENIC E. COLI (VIRULENCE MECHANISMS OF BACTERIAL PATHOGENS, 9/12-15/99, AMES, IA) - (Abstract Only) - (15-Sep-99)

SEMI-AUTOMATED FLUORESCENT (TAQMAN) PCR ASSAYS FOR RAPID DETECTION OF ESCHERICHIA COLI O157:H7 AND OTHER SHIGA TOXIGENIC E. COLI (STEC) - (Abstract Only) - (03-Jun-99)

SEMI-AUTOMATED FLUOROGENIC PCR ASSAYS (TAQMAN) FOR RAPID DETECTION OF ESCHERICHIA COLI O157:H7 AND OTHER SHIGA TOXIGENIC E. COLI - (Peer Reviewed Journal) - (13-May-99)

DETECTION OF MULTIRESISTANT SALMONELLA TYPHIMURIUM DT104 USING MULTIPLEX AND FLUOROGENIC PCR - (Peer Reviewed Journal) - (10-Mar-99)

NEW HIGH-TECH TESTS MORE QUICKLY DETECT A KEY ORGANISM THAT CAN CAUSE FOOD POISONING - (Other) - (31-Dec-98)

POSSIBLE TARGETS FOR E. COLI O157:H7 VACCINES IN CATTLE - (Abstract Only) - (03-Dec-98)