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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Food Composition and Methods Development Laboratory » Research » Publications at this Location » Publication #70522
Title: EFFECT OF LIPID EXTRACTION METHODS ON TOTAL DIETARY FIBER AND FIBER POLYSACCHARIDE CONTENTS OF SELECTED NUTS AND SEEDS

Author
item Li, Betty
item ZHAO, ZHENKUN - 1235-15-00
item JEKOT, JILL - DIONEX CORPORATION

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/26/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Three extraction methods were used to remove lipid materials from eight edible nuts and seeds before analyzing for their total dietary fiber (TDF) and nonstarch polysaccharide (NSP) contents. Portions of ground materials were extracted by: 1)n-hexane, followed by 80% methanol 2) n-hexane and acetic acid (95:5 v:v) 3) supercritical fluid - carbon dioxide. Defatted samples were gelatinized in water and incubated with amyloglucosidase; 95% ethanol was added to the hydrolyzates, and the residues were collected on tared glass crucibles. TDF was calculated according to a simplified enzymatic-gravimetric method developed in our laboratory, and NSP was de- termined using a gas-liquid chromatographic procedure modified by Englyst and coworkers. Dietary fiber values for all nuts and seeds using the three extraction methods ranged from 3.47 g/100g for cashew to 27.47 g/100g for sunflower seed. Nonstarch polysaccharide value ranged from 2.43 g/100g for pecan to 5.56 g/100g for peanuts. With the exception of sunflower seed, superciritical fluid extracted samples had lower TDF content but similar amounts of NSP compared to the samples extracted by the other methods.