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ARS Home » Southeast Area » Oxford, Mississippi » Natural Products Utilization Research » Research » Publications at this Location » Publication #300458
Title: Characterization of steroidal saponins from Dioscorea villosa and D Cayennensis using ultrahigh performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry

Author
item AVULA, BHARTHI - University Of Mississippi
item WANG, YAN-HONG - University Of Mississippi
item WANG, MEI - University Of Mississippi
item ALI, ZULFIQAR - University Of Mississippi
item SMILLIE, TROY - University Of Mississippi
item ZWEIGENBAUM, JERRY - University Of Mississippi
item KHAN, KHAN - University Of Mississippi

Submitted to: Planta Medica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/4/2014
Publication Date: 2/7/2014
Citation: Avula, B., Wang, Y., Wang, M., Ali, Z., Smillie, T.J., Zweigenbaum, J., Khan, K. 2014. Characterization of steroidal saponins from Dioscorea villosa and D Cayennensis using ultrahigh performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry. Planta Medica. 80:321-329.

Interpretive Summary: UHPLC-QTOF-MS for the characterization of saponins from rhizomes Dioscorea species and dietary supplements. 21 saponins were detected in the crude extracts of two Dioscorea species. The fragments revealed the characteristic cleavage of glycosidic bonds. Cleavages provided structural information about the sugars. Principal component analysis (PCA), showed that Dioscorea species and commercial products were easily distinguished. From this a partial least squares model was constructed to determine what species are in different products.

Technical Abstract: Steroidal saponins were reported to be the major physiologically active constituents in yams. The structural characteristics of steroidal saponins in methanolic extracts from dried rhizomes of two Dioscorea species (D. villosa L. and D. cayenensis Lam.) and dietary supplements have analyzed using UHPLC/QTOF-MS in both negative and positive ion modes. Principal component analysis (PCA) was used to interpret 9 samples of D. villosa, 3 samples of D. caynensis, and 5 dietary products claiming to contain D. villosa on the chemical parameters in order to investigate and visualize the homogeneity of the samples