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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Cotton Fiber Bioscience Research » Research » Publications at this Location » Publication #296287
Title: Linkage map construction and QTL analysis of agronomic and fiber quality traits in cotton

Author
item GORE, MICHAEL - Cornell University
item Fang, David
item Poland, Jesse
item ZHANG, JINFA - New Mexico State University
item Percy, Richard
item CANTRELL, ROY - Monsanto Corporation
item Thyssen, Gregory

Submitted to: The Plant Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/28/2013
Publication Date: 3/28/2014
Publication URL: https://handle.nal.usda.gov/10113/59642
Citation: Gore, M.A., Fang, D.D., Poland, J.A., Zhang, J., Percy, R.G., Cantrell, R.G., Thyssen, G.N. 2014. Linkage map construction and QTL analysis of agronomic and fiber quality traits in cotton. The Plant Genome. 7(1):1-10.

Interpretive Summary: Production of high quality cotton is essential for the US cotton industry. The superior fiber properties of Pima cotton (Gossypium barbadense) serve as a source of novel variation for improving fiber quality in American Upland cotton (G. hirsutum), but introgression from Pima cotton has been largely unsuccessful due to hybrid breakdown and a lack of genetic and genomic resources. In an effort to overcome these limitations, we constructed a linkage map and conducted genetic analysis of 10 agronomic and fiber quality traits in a recombinant inbred mapping population derived from a cross between TM-1, an Upland cotton line, and NM24016, an elite Upland cotton line with stabilized introgression from Pima cotton. The linkage map consisted of 429 simple-sequence repeat (SSR) and 412 single-nucleotide polymorphism (SNP) marker loci that covered half of the tetraploid cotton genome. Notably, the 841 marker loci were unevenly distributed among the 26 chromosomes of tetraploid cotton. The 10 traits evaluated on the TM-1×NM24016 population in a multi-environment trial were highly heritable and most of the fiber traits showed considerable transgressive variation. Through the quantitative trait analysis, we identified a total of 28 quantitative trait locus (QTLs) associated with the 10 traits. Our study provides a novel resource that can be used by breeders and geneticists for the genetic improvement of agronomic and fiber quality traits in Upland cotton.

Technical Abstract: The superior fiber properties of Gossypium barbadense L. serve as a source of novel variation for improving fiber quality in Upland cotton (G. hirsutum L.), but introgression from G. barbadense has been largely unsuccessful due to hybrid breakdown and a lack of genetic and genomic resources. In an effort to overcome these limitations, we constructed a linkage map and conducted a quantitative trait locus (QTL) analysis of 10 agronomic and fiber quality traits in a recombinant inbred mapping population derived from a cross between TM-1, an Upland cotton line, and NM24016, an elite G. hirsutum line with stabilized introgression from G. barbadense. The linkage map consisted of 429 simple-sequence repeat (SSR) and 412 single-nucleotide polymorphism (SNP) marker loci that covered half of the tetraploid cotton genome. Notably, the 841 marker loci were unevenly distributed among the 26 chromosomes of tetraploid cotton. The 10 traits evaluated on the TM-1×NM24016 population in a multi-environment trial were highly heritable and most of the fiber traits showed considerable transgressive variation. Through the QTL analysis, we identified a total of 28 QTLs associated with the 10 traits. Our study provides a novel resource that can be used by breeders and geneticists for the genetic improvement of agronomic and fiber quality traits in Upland cotton.