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United States Department of Agriculture

Agricultural Research Service

Research Project: ENVIRONMENTALLY SOUND MANURE MANAGEMENT FOR REDUCTION OF GAS EMISSIONS, NUTRIENTS, AND PATHOGENS

Location: Agroecosystem Management Research

Title: Fate and transport of antimicrobial and antimicrobial resistance genes in soil and runoff following land application of swine slurry

Authors
item Joy, Stacey -
item Bartelt-Hunt, Shannon -
item Snow, Daniel -
item Gilley, John
item Woodbury, Bryan
item Parker, David -
item Marx, David -
item Li, Xu -

Submitted to: Journal of Environmental Science and Technology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 17, 2013
Publication Date: N/A

Interpretive Summary: Animal production is an important source of antimicrobials and antimicrobial resistant bacteria to the environment. Antimicrobials may allow resistant bacteria to proliferate in animal manure and, therefore, antimicrobials and antimicrobial resistance genes may be transported to surface and ground water after manure is land applied as fertilizer. The objective of this study was to investigate the fate and transport of antimicrobials and antimicrobial resistance genes in soil and surface runoff following land application of swine slurry. Rainfall simulation tests were conducted after swine slurry containing three selected antimicrobials was applied via broadcast (surface application), incorporation, or injection methods. Both antimicrobials and antimicrobial resistance genes were detected in runoff after swine slurry application, indicating the potential for transport of these constituents to surface waters. Antimicrobial and antimicrobial resistance genes concentrations in runoff decreased over the three-day experiment. No significant vertical transport of antimicrobial resistance genes was detected in the soil. The transport of antimicrobials is affected by manure application methods and the timing of rainfall, as lower antimicrobial concentrations were observed in runoff after the third runoff event.

Technical Abstract: Animal production is an important source of antimicrobials and antimicrobial resistant bacteria to the environment. Antimicrobials may allow resistant bacteria to proliferate in animal manure, and therefore antimicrobials and antimicrobial resistance genes (ARGs) may be transported to surface and ground water after manure is land applied as fertilizer. The objective of the study was to investigate the fate and transport of antimicrobials and ARGs in soil and surface runoff following land application of swine slurry. Rainfall simulation tests were conducted after swine slurry containing bacitracin, chlortetracycline, or tylosin, was applied via broadcast (surface application), incorporation, or injection methods. Both antimicrobials and ARGs were detected in runoff after swine slurry application, indicating the potential for transport of these constituents to surface waters. Antimicrobial and ARG concentrations in runoff decreased over the three-day experiment. No significant vertical transport of ARGs was detected in the soil. The transport of antimicrobials is affected by manure application methods, and the timing of rainfall, as lower antimicrobial concentrations were observed in runoff after the third runoff event.

Last Modified: 4/18/2014
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