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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #153296
Title: COLD STORAGE AND CRYOPRESERVATION OF HOPS (HUMULUS L.) SHOOT CULTURES THROUGH APPLICATION OF STANDARD PROTOCOLS

Author
item Reed, Barbara
item OKUT, NESE - YUZUNCU YIL UNIV
item D Anchino, Jeff
item NARVER, LEE - USDA
item DE NOMA, JEANINE - USDA

Submitted to: CryoLetters
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/8/2003
Publication Date: 12/20/2003
Citation: REED, B.M., OKUT, N., D ACHINO, J., NARVER, L., DE NOMA, J. COLD STORAGE AND CRYOPRESERVATION OF HOPS (HUMULUS L.) SHOOT CULTURES THROUGH APPLICATION OF STANDARD PROTOCOLS. CRYOLETTERS. v. 24. 2003. p.389-396

Interpretive Summary: The USDA-ARS National Clonal Germplasm Repository stores the global diversity of hops for the US Plant Germplasm System. Most hops are stored as plants in the field. Tissue culture storage and cryopreservation in liquid nitrogen are now considered excellent ways to store plants for decades. Developing a new technique for every individual type of plant in a large multi-crop collection can be a very time consuming activity. We propose that standard cold storage and cryopreservation techniques used for other temperate plants would be successful for additional crops. This study was initiated to determine if a large collection of hops could be successfully stored with techniques developed for pears and strawberries. We found that 70 different types of hops could be stored at refrigerator temperatures (45 °F) for an average of 14 months with a strawberry technique. Small shoot tips of hops could be frozen and stored in liquid nitrogen (-320 °F) following a technique developed for pear and recovery after thawing was high, up to 80%. Application of a well-tested standard technique can provide a quick start for storing additional germplasm collections.

Technical Abstract: The USDA-ARS National Clonal Germplasm Repository (NCGR) stores the global diversity of Humulus for the US Plant Germplasm System as trellised plants in a field genebank. In vitro storage and cryopreservation are now considered excellent ways to provide medium and long-term storage for plant collections. Developing a new cryopreservation or cold storage protocol for every accession or genus of large multi-crop collections can be a very time consuming and long-term activity. We propose that standard cold storage and cryopreservation techniques used for other temperate crop genera would be successful for additional crops with few modifications. This study was initiated to determine if a large collection of hops germplasm could be successfully stored with techniques developed for unrelated genera. In this study we characterized the response of diverse Humulus genotypes to in vitro storage under low light at 4 °C following techniques used for strawberry and mint plants, and cryopreservation in liquid nitrogen by slow cooling with a pear protocol. The average storage time without transfer for the 70 genotypes evaluated was 14 + 3.5 months with a range of 6 to 26 months. Mean recovery of cryopreserved shoot tips of accessions with 1-wk cold acclimation was 41% + 18 and increased to 54% + 13 with 2-wk cold acclimation. This demonstrates that application of a well-tested standard technique can provide a quick start for storing additional germplasm collections.