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ARS Home » Research » Publications at this Location » Publication #89245
Title: RESPONSE OF DEHYDRINS TO DROUGHT, LOW TEMPERATURE, AND ABA TREATMENT IN WHOLE PLANTS AND CELL SUSPENSION CULTURES OF BLUEBERRY

Author
item PANTA, GANESH - UNIVERSITY OF GEORGIA
item PARMENTIER, CECILE - OICD
item Rowland, Lisa

Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: 1/16/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Previously three dehydrins of 65, 60, and 14 kD were identified as the predominant proteins present in cold acclimated blueberry floral buds. Levels were shown to increase with cold acclimation and decrease with deacclimation and resumption of growth. Recently, a dehydrin cDNA clone was isolated and sequenced, and shown to hybridize to messages likely to encode all three dehydrins. In the present study, expression of dehydrins was examined in blueberry cultivars in response to drought and low temperature treatment and in cell suspension cultures in response to low temperature and ABA treatment. During 32 days of drought stress, relative shoot water content dropped to 51 to 90% depending upon cultivars. For cold stress experiments, cultivars with different chilling requirements and levels of cold hardiness were kept at 4 degrees C for five weeks. Cell suspension cultures were held at 4 degrees C for up to two weeks. For ABA experiment, ABA concentrations ranging from 10-3 to 10-7 M were used. Dehydrins were monitored in response to various treatments at RNA and proteins levels using the cDNA clone and antisera raised against the dehydrins. Interestingly, a previously uncharacterized 30 kD dehydrin was found to be the major low temperature- and ABA responsive protein in cell suspension cultures.