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United States Department of Agriculture

Agricultural Research Service

Title: Transformation-Mediated Gene Disruption of the Gene, Nip1, Encoding a 24 Kda Necrosis Inducing Protein in Fusarium Oxysporum F. Sp. Erythroxyli

item Birkhold, Patricia
item Luster, Douglas
item Bailey, Bryan

Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: April 26, 1997
Publication Date: N/A

Technical Abstract: A 24-kDa protein, NIP1, from Fusarium oxysporum has been shown to elicit ethylene production and necrosis in leaves of Erythroxylum coca. Many dicotyledonous plants have also been shown to induce similar responses when treated with the elicitor/toxin. In order to determine if this elicitor/toxin is important in pathogenesis of E. coca or induces resistance in dicots, transformation-mediated gene disruption is being use to disrupt Nip1. Also, constructs are being made to overexpress the elicitor/toxin gene in F. oxysporum isolate EN-4 to determine if pathogenesis can be altered. The strains containing the disruption and overexpression of the Nip1 gene will be analyzed by Southern, northern, and western blot analysis, as well as root dip bioassays. These experiments will help to determine if the 24 kDa elicitor/toxin plays a role in disease development of F. oxysporum on E. coca or may be used with biocontrol agents to induce plant defense responses.

Last Modified: 4/22/2015
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