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ARS Home » Plains Area » Houston, Texas » Children's Nutrition Research Center » Research » Publications at this Location » Publication #62154
Title: INTRINSIC 42 CA-LABELLING OF GREEN BEAN PODS FOR USE IN HUMAN BIOAVAILIBILITY STUDIES

Author
item Grusak, Michael
item PEZESHGI, SHAHRBANU
item O'BRIEN, KIMBERLY
item ABRAMS, STEVEN

Submitted to: Journal of the Science of Food and Agriculture
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/19/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A hydroponic-growth procedure which enables the intrinsic 42Ca- labeling of green beans (Phaseolus vulgaris (L)) has been developed. Whole-plant Ca influx was measured from planting through the period of pod expansion for a crop of 60 plants grown hydroponically in a 3 m2 area. Total Ca influx was 10.1 mmol plant-1; 35% of this influx occurred during the period of pod growth. Plants were labeled with 42Ca via the nutrient solution during the period of pod expansion; isotopic composition of harvested pods was nearly identical to that of the administered solution. Recovery of isotopic tracer at harvest was: pods, 8.4%; leaves, 61.1%; stems, 17.1%, roots, 9.0%. Pod yield was 98.8 g FW plant-1. 42Ca-labeled green beans can be used to safely investigate Ca bioavailability in humans.