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Title: SIMPLE SEQUENCE REPEAT-BASED SINGLE PRIMER AMPLIFICATION OF GENOMIC DNA IN RANDOM BRED POPULATIONS OF TURKEYS AND CHICKENS

Author
item SMITH, E - TUSKEGEE UNIVERSITY
item RAY, S - TUSKEGEE UNIVERSITY
item DRUMMOND, P - TUSKEGEE UNIVERSITY
item Bakst, Murray
item TEUSCHER, C - BRIGHAM YOUNG UNIVERSITY

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/2/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: New techniques in molecular biology are being developed for the genetic selection of poultry. In one type of selection strategy, genetic markers, which can be short repeating sequences in the DNA that are always associated with specific but complex genetic traits of economic importance, need to be identified. Using different approaches to molecular analysis, difference short repeating sequences of DNA were found to be unique to the turkey and chicken. These data will contribute to the development of a more detailed genetic maps of the chicken and turkey.

Technical Abstract: The characteristics of polymerase chain reaction (PCR) amplification products based on single primers of di-, tri- and tetra-nucleotide simple sequence repeats (SSR) in turkeys and chickens were evaluated. A total of 60 turkeys and 30 chickens were included in the PCR typing by one di-, 6 tri- and 8 tetra-nucleotide SSR primers. Annealing temperature and PCR conditions were optimized to maximize amplification of multiple bands by each SSR primer in both chickens and turkeys. Amplification products by (TG)14 and (CTG)5 repeats were smears in both turkeys and chickens. Two primers, (CTA)7 and (TATC)5 did not amplify any product in both species. A total of 63 bands in turkeys and 74 in chickens were amplified, of which only 11% in turkeys and 16% in chickens were polymorphic. About 90% of the amplified fragments were species-specific in turkeys and chickens. These results suggest that single SSR primer amplification reveals low inter- repeat length polymorphism in both turkeys and chickens. The high frequency of species-specific amplification products suggests that single SSR primer analysis of microsatellites may be useful in the study of phylogenetic relationships between turkeys and chickens. Additionally, the fragments described would provide additional markers in the development of genetic maps for both turkeys and chickens.