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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #323146
Title: Genome-wide association mapping of barley yellow dwarf virus tolerance in spring oat (Avena sativa L.)

Author
item FORESMAN, BRADLEY - University Of Illinois
item OLIVER, REBEKAH - North Dakota State University
item JACKSON, ERIC - General Mills, Inc
item Chao, Shiaoman
item ARRUDA, MARCIO - University Of Illinois
item KOLB, FREDERIC - University Of Illinois

Submitted to: PLOS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/27/2016
Publication Date: 5/13/2016
Publication URL: http://handle.nal.usda.gov/10113/62916
Citation: Foresman, B.J., Oliver, R.E., Jackson, E.W., Chao, S., Arruda, M.P., Kolb, F.L. 2016. Genome-wide association mapping of barley yellow dwarf virus tolerance in spring oat (Avena sativa L.). PLoS ONE. 11(5):e0155376. doi: 10.1371/journal.pone.0155376.

Interpretive Summary: Oat is an important cereal crop grown worldwide with nutritional benefits for both livestock and humans. Barley yellow dwarf (BYD) is one of the most destructive diseases of cereal crops, including oat, and is caused by barley yellow dwarf viruses (BYDVs). The most effective way to control BYD is by planting tolerant cultivars. Traditionally, phenotyping for BYDV has been the only reliable method for screening breeding material for tolerance, which tends to be a time consuming process. The recent development of high-density molecular marker technology in oat provided an option of allowing tolerant lines to be selected more efficiently. The objectives of this study were to evaluate a panel of 428 spring oat lines and identify genes affecting disease tolerance using a high-density oat marker array. Results showed two genes located on two different chromosomes had large genetic effects on BYDV tolerance. Altogether 259 lines were found carrying both genes that greatly improved the levels of tolerance to the disease. From a breeding perspective, identifying the most tolerant lines is important because this group can be selected to move forward in a breeding program or could be used as parents to improve other lines without tolerance.

Technical Abstract: Barley yellow dwarf (BYD) is one of the most destructive diseases of cereal crops worldwide. Barley yellow dwarf viruses (BYDVs) are responsible for BYD and affect many cereals including oat (Avena sativa L.). Until recently, the molecular marker technology in oat has not allowed for many marker-trait association studies to determine the genetic mechanisms for tolerance. A genome-wide association study (GWAS) was performed on 428 spring oat lines using a recently developed high-density oat single nucleotide polymorphism (SNP) array as well as a SNP-based consensus map. Marker-trait associations were performed using a Q-K mixed model approach to control for population structure and relatedness. Six significant SNP-trait associations representing two QTL were found on chromosomes 3C and 18D. This is the first report of BYDV tolerance QTL on chromosome 3C and 18D. Haplotypes using the two QTL were evaluated and distinct classes for tolerance were identified based on the number of favorable alleles. A large number of lines carrying both favorable alleles were observed in the panel.