Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay

Authors: Yeh, Hung-Yueh; SERRAND, KATHERINE - University Of Puerto Rico; SILVESTRY, AIMEE - University Of Puerto Rico; Buhr, Richard - Jeff

Submitted to: Journal of Microbiological Methods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/14/2016
Publication Date: 3/1/2016
Citation: Yeh, H., Serrand, K.V., Silvestry, A., Buhr, R.J. 2016. Production of recombinant Salmonella flagellar protein, FlgK, and its uses in detection of anti-Salmonella antibodies in chickens by automated capillary immunoassay. Journal of Microbiological Methods. 122:(3)27-32.

Interpretive Summary: Traditional immunological techniques for detection of a specific protein are very tedious, labor-intensive and time-consuming. A new technology called 'Simple Western" based on capillary electrophoresis has been developed by Protein Simple™. This article describes the use of recombinant Salmonella FlgK protein (flagellar hook-filament junction protein at hook) as a probe to evaluate whether chicken sera contained antibodies against FlgK using the Simple Western immunoassay.

Technical Abstract: Conventional immunoblot assays have been a very useful tool for specific protein identification in the past several decades, but are tedious, labor-intensive and time-consuming. An automated capillary electrophoresis-based immunoblot assay called "Simple Western" has recently been developed that enables the protein separation, blotting and detection in an automatic manner. This communication describes the use of recombinant Salmonella FlgK protein (flagellar hook-filament junction protein at hook) as a probe to evaluate whether chicken sera contained antibodies against FlgK using the Simple Western immunoassay.