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ARS Home » Northeast Area » Beltsville, Maryland (BHNRC) » Beltsville Human Nutrition Research Center » Food Composition and Methods Development Laboratory » Research » Publications at this Location » Publication #317232
Title: Fingerprinting profile of polysaccharides from Lycium barbarum using multiplex approaches and chemometrics

Author
item LU, WEI - China Pharmaceutical University
item XU, JINNAN - China Pharmaceutical University
item ZHU, RUI - China Pharmaceutical University
item ZHU, YIQING - China Pharmaceutical University
item ZHAO, YANG - University Of Maryland
item Chen, Pei
item PAN, CHUN - Southern University
item YAO, WENBING - China Pharmaceutical University
item GAO, XIANGDONG - China Pharmaceutical University

Submitted to: International Journal of Biological Macromolecules
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/3/2015
Publication Date: 5/3/2015
Citation: Lu, W., Xu, J., Zhu, R., Zhu, Y., Zhao, Y., Chen, P., Pan, C., Yao, W., Gao, X. 2015. Fingerprinting profile of polysaccharides from Lycium barbarum using multiplex approaches and chemometrics. International Journal of Biological Macromolecules. 3:78:230-237.

Interpretive Summary: Fingerprints of Lycium barbarum ("gou qi") ) from different locations and varieties were obtained using 1)ultraviolet-visible spectra (UV), 2) high performance size-exclusion chromatography (HPSEC), 3) fourier-transform infrared spectroscopy (FT-IR), and 4) pre-column derivatization high-performance liquid chromatography (PCD-HPLC) for the analysis of Lycium barbarum polysaccharides (LBPs). Chememetris was used for the analysis of the fingerprinting profiles. The results indicated fingerprinting techniques used were simple and robust for quality control purposes. In addition, fingerprinting techniques combined with chemometrics did a good job to classify the cultivation locations of LBPs samples. Four monosaccharides (galacturonic acid, glucose, galactose and arabinose) could be selected as markers to distinguish different samples.

Technical Abstract: Techniques including ultraviolet-visible spectra (UV), high performance size-exclusion chromatography (HPSEC), fourier-transform infrared spectroscopy (FT-IR) and pre-column derivatization high-performance liquid chromatography (PCD-HPLC) were used in the fingerprinting analysis of Lycium barbarum polysaccharides (LBPs) from different locations and varieties. Multiple fingerprinting profiles were used to evaluate the similarity and classification of different LBPs with the help of chemometrics. The results indicated that sixteen batches of LBPs had good consistency, and fingerprinting techniques were simple and robust for quality control of LBPs as well as related products. In addition, fingerprinting techniques combined with chemometrics could also be used to identify different cultivation locations of LBPs samples. Finally, four monosaccharides (galacturonic acid, glucose, galactose and arabinose) and the absorptions of stretching vibration of ester carbonyl groups as well as N-H variable angle vibration of –CONH– could be selected as herbal markers to distinguish different samples.