Skip to main content
ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Invasive Insect Biocontrol & Behavior Laboratory » Research » Publications at this Location » Publication #315191
Title: Genome sequence of an Adoxophyes orana granulovirus (AdorGV) occlusion body morphology mutant

Author
item NAKAI, MADOKA - Tokyo University Of Agriculture & Technology
item Harrison, Robert - Bob
item UCHIDA, HARUAKI - Tokyo University Of Agriculture & Technology
item KUNIMI, YASUHISA - Tokyo University Of Agriculture & Technology

Submitted to: Japanese Journal of Applied Entmology and Zoology
Publication Type: Abstract Only
Publication Acceptance Date: 3/10/2015
Publication Date: 3/26/2015
Citation: Nakai, M., Harrison, R.L., Uchida, H., Kunimi, Y. 2015. Genome sequence of an Adoxophyes orana granulovirus (AdorGV) occlusion body morphology mutant. Japanese Journal of Applied Entmology and Zoology. p.131.

Interpretive Summary:

Technical Abstract: - Genome sequence of an Adoxophyes orana granulovirus (AdorGV) occlusion body morphology mutant Baculoviruses package virus particles in a crystalline matrix of viral protein to form occlusion bodies. Occlusion bodies are used in biological control because the crystalline matrix protects the viral particles in from environmental decay. The genes which determine the form (shape and size) of the occlusions bodies are not clear. An isolate of Adoxophyes orana granulovirus (AdorGV) was isolated that produced occlusion bodies exhibiting an unusual shape and size. Ultrastructural observations revealed that its OBs were significantly larger (up to 2 µm in diameter) and cuboidal in shape, compared to the ellipsoidal shape of about 0.5µm typically observed for this virus. To identity genes and mutations that correlate with this unusual OB morphology, the entire genome of the morphology mutant was sequenced and found to be 99,507 bp in size. The morphology mutant genome exhibited 99.7% nucleotide sequence identity with the standard isolates, with 66 of the 121 putative ORFs being 100% identical between viruses with mutant and wild-type OB morphology. Mutations in genes involved in OB morphogenesis, such as pep-p10, were observed in the OB morphology mtuant.