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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Bioenergy Research » Research » Publications at this Location » Publication #311997
Title: Savagea faecisuis gen. nov., sp. nov., a tylosin- and tetracycline-resistant bacterium isolated from a swine-manure storage pit

Author
item Whitehead, Terence
item JOHNSON, CRYSTAL - University Of Oklahoma
item PATEL, NISHA - University Of Oklahoma
item Cotta, Michael
item MOORE, EDWARD R - University Of Oklahoma
item LAWSON, PAUL - University Of Oklahoma

Submitted to: Antonie Van Leeuwenhoek
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/3/2015
Publication Date: 5/19/2015
Publication URL: http://handle.nal.usda.gov/10113/62173
Citation: Whitehead, T.R., Johnson, C.N., Patel, N.B., Cotta, M.A., Moore, E.R.B., Lawson, P.A. 2015. Savagea faecisuis gen. nov., sp. nov., a tylosin- and tetracycline-resistant bacterium isolated from a swine-manure storage pit. Antonie van Leeuwenhoek. 108(1):151-161.

Interpretive Summary: Antimicrobial compounds have been commonly used as feed additives for domestic animals to reduce infection and promote growth. Recent reports have suggested such feeding practices may result in increased microbial resistance to antibiotics, which can have an impact on human health. While many investigations have centered on antibiotic resistance in known pathogens, less attention has been directed towards investigating antibiotic resistance in the commensal bacteria found in the gastrointestinal tract and stored manure of domestic animals. We now report on the identification and scientific naming of a new species of antibiotic resistant aerobic bacterium, Savagea faecisuis, isolated from a swine manure storage pit. This information will be of use to other researchers and those interested in antibiotic resistance in animal livestock.

Technical Abstract: A polyphasic taxonomic study using morphological, biochemical, chemotaxonomic and molecular methods was performed on three strains of an unknown Gram-positive staining, nonspore-forming, motile aerobic rod-shaped bacterium resistant to tetracycline and tylosin isolated from a swine-manure storage pit. On the basis of 16S rRNA gene sequence analyses, it was confirmed that these isolates were highly related to each other and formed a hitherto unknown linage within the Planococcaceae. In particular, pairwise analysis of the 16S rRNA gene demonstrated that the novel organism was most closely related to members of the genus Sporosarcina (92.8-94.5%), Pyschrobacillus (93.5-93.9%) and Paenisporosarcina (93.3-94.5%). The predominant fatty acids consist of iso-C15:0 and iso-C17:1 '10c and the G +C mol % is 41.8. Based on biochemical, chemotaxonomic, and phylogenetic evidence, it is proposed that the unknown bacterium be classified as a novel genus and species, Savagea faecisuis gen nov., sp. nov. The type strain is Con12T (=CCUG 63563T = NRRL B-59945T).