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Title: Molecular sequence dataset to identify metobolic-based pyrethroid resistance in the horn fly, Haematobia irritans.

Author
item Guerrero, Felicito
item CAMERON, CONNOR - National Center For Genome Resources
item FOIL, LANE - Louisiana State University
item THADEU DE BARROS, ANTONIO - Embrapa
item CROW, JOHN - National Center For Genome Resources
item RETZEL, ERNIE - National Center For Genome Resources

Submitted to: Brazilian Congress of Veterinary Parasitology
Publication Type: Abstract Only
Publication Acceptance Date: 4/7/2012
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Insecticide resistance can arise through several mechanisms broadly classified as target site, metabolic, or reduced penetration. Penetration resistance can arise through alterations in the ability of an insecticide to penetrate or otherwise enter an individual that is treated with insecticide. Target site resistance exists when an allele of the gene coding for the target molecule attacked by the insecticide has an amino acid mutation that confers resistance. Metabolic resistance occurs through changes in the ability of an individual to detoxify or sequester an insecticide. The molecular aspects of metabolic resistance are not yet well-defined in the horn fly. From studies in other insects, the enzyme families known as cytochrome P450s, esterases, and glutathione S-transferases are generally involved in metabolic resistance and a dataset of transcripts encoding these proteins in the horn fly is now available. As in other insects, each of these are large gene families in the horn fly with 37, 51, and 16 individual members, respectively, putatively identified at this time. It is likely that other members of these gene families remain to be identified in the horn fly and further transcriptome studies should enable the identification of the complete horn fly metabolome. Populations of horn flies from Brazil and the United States that appear to possess significant pyrethroid resistance through metabolic mechanisms have been identified though bioassay techniques. A protocol is discussed whereby the molecular basis of the metabolic resistance in the populations might be identified. USDA is an equal opportunity provider and employer.