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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #236040
Title: Single-Antigen Serological Testing for Bovine Tuberculosis

Author
item GREEN, LAWRENCE - PRITEST INC.
item JONES, CYNTHIA - PRITEST INC.
item SHERWOOD, ANNE - PRITEST INC.
item GARKAVI, INNA - PRITEST INC.
item CANGELOSI, GERARD - PRITEST INC.
item Thacker, Tyler
item Palmer, Mitchell
item Waters, Wade
item RATHE, CHRIS - PRITEST INC.

Submitted to: Clinical and Vaccine Immunology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/6/2009
Publication Date: 9/1/2009
Citation: Green, L.R., Jones, C.C., Sherwood, A., Garkavi, I., Cangelosi, G.A., Thacker, T.C., Palmer, M.V., Waters, W.R., Rathe, C. 2009. Single-Antigen Serological Testing for Bovine Tuberculosis. Clinical and Vaccine Immunology. 16(9):1309-1313.

Interpretive Summary: Despite highly successful eradication efforts in several countries, tuberculosis of cattle remains a serious health concern worldwide. Recent outbreaks of tuberculosis in Michigan, Minnesota, California, Texas, and New Mexico demonstrate that the disease is far from eliminated from the United States. Improved techniques are needed for detection of infected cattle. In this study, a new test was developed and tested for the specific diagnosis of tuberculosis infection of cattle. Responses by cattle infected with the tubercle bacillus were distinguishable from responses by cattle infected with closely related bacteria. Knowledge obtained from this study will enable more accurate detection of cattle with tuberculosis, thereby, enhancing the tuberculosis eradication program.

Technical Abstract: Antibody responses are useful indicators of Mycobacterium bovis infection of cattle. Tests for serological responses often use panels of multiple M. bovis antigens as detection probes. This is recommended because responses to single antigens may be too variable for consistent diagnosis. However, the use of multiple antigens increases costs and the risk of cross reactivity with antibodies directed against orthologous antigens expressed by other species. An alternative approach is to use a single antigen in a detection system with high analytical sensitivity. This approach is used in the SeraLyte-TBTM system, a sensitive chemiluminescent test for antibodies that bind to ferrite particles coated with the MPB83 antigen. SeraLyte testing was conducted in blinded fashion on frozen sera (N = 90 samples) collected from experimentally infected and control cattle (N = 71 animals). The sample included sera from animals infected with M. bovis (N = 27), M. kansasii (N = 4), M. avium subspecies paratuberculosis (N = 11), M. avium subspecies avium (N = 12) and uninfected animals (N = 17). As with all serological tests that utilize MPB83, reactivity was expected in M. bovis and M. kansasii-infected animals, but not in M-avium-infected and uninfected animals. Upon unblinding, sensitivity relative to known infection was 90% among the 71 individual animals, and 92% among the 90 individual samples tested. Specificity was 100%. The results indicate that SeraLyte-TB(TM) has sufficient sensitivity for detecting infection based on serological response to a single antigen.