|Tamborindeguy, C. - CORNELL UNIVERSITY|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: March 1, 2008
Publication Date: July 1, 2008
Citation: Tamborindeguy, C., Gray, S.M. 2008. Analysis of Cyclophilin involvement in CYDV-RPV transmission by Schizaphis graminum. Phytopathology. 98:S154. Technical Abstract: Luteoviruses are transmitted by aphids in a circulative, nonpropogative manner requiring the virus to be actively transported across cell membranes and through cell cytoplasm. Using proteomic analyses we have identified two forms of Cyclophilin in a F2 Schizaphis graminum population segregating for the ability to transmit Cereal yellow dwarf virus (CYDV)-RPV. Cyclophilin is a peptidyl-prolyl cis-trans-isomerase and has been involved in several functions including macromolecular transport. The cyclophilin gene sequence was determined for several S. graminum genotypes that differ in vector competency and also differing in which barrier (hindgut or salivary gland) is operating in the nonvector genotypes. A single point mutation changing a C to G is correlated with genotypes that are unable to transmit virus. The cyclophilin that predominates in the vector genotypes is able to bind to purified virus particles in co-immunoprecipitation assays. Continuing efforts are focused on functional assays to directly determine the involvement of cyclophilin in CYDV movement in the aphid.