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Title: Expressed gene sequences from egg and larval stages of the horn fly, Haematobia irritans

Author
item Guerrero, Felicito
item Dowd, Scot

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 1/14/2008
Publication Date: 1/14/2008
Citation: Guerrero, F., Dowd, S.E. 2008. Expressed gene sequences from egg and larval stages of the horn fly, Haematobia irritans. National Center for Biotechnology Information (NCBI). Available: http://www.ncbi.nlm.nih.gov. Assession numbers FD449556-FD457982 (Egg) and FD457982-FD466257 (Larvae)

Interpretive Summary: The horn fly, Haematobia irritans, is a blood-feeding parasite of cattle which is a controlled with good animal husbandry practices and the application of pesticides. Pesticide resistance is a developing problem for producers and has a negative economic impact upon production. We established a large sequencing project to identify genes from the horn fly. We hope to identify genes which are involved in the development of pesticide resistance and also identify genes which might prove candidate targets for the design of novel control technologies. In this project, we sequenced 8,427 and 8,275 expressed genes from the egg and first instar larvae lifestages of the horn fly. These sequences have been submitted and published under accession numbers FD449556-FD457982 (Egg) and FD457983-FD466257 (Larvae) in the National Center for Biotechnology Information Public Database.

Technical Abstract: We used an EST approach to initiate a study of the genome of the horn fly, Haematobia irritans and have isolated and sequenced 8,427 and 8,275 expressed genes from the egg and first instar larvae lifestages. Normalized cDNA libraries from eggs and first instar larvae from a field population of horn flies were the source of the ESTs. These sequences were used to search publicly accessible databases to identify sequence similarity to known genes and assign putative function to the horn fly genes. The ESTs have been submitted and published under accession numbers FD449556-FD457982 (Egg) and FD457983-FD466257 (Larvae) in the National Center for Biotechnology Information Public Database.