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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Publications at this Location » Publication #214535
Title: Expression of the N-terminal 99 Amino Acids of Yeast Ribosomal Protein L3 in Transgenic Wheat Confers Resistance to Fusarium Head Blight

Author
item DI, RONG - RUTGERS UNIVERSITY, NJ
item Blechl, Ann
item DILL-MACKY, RUTH - UNIV. OF MINNESOTA
item TORTORA, A. - RUTGERS UNIVERSITY, NJ
item TUMER, NILGUN - RUTGERS UNIVERSITY, NJ

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/27/2007
Publication Date: 4/1/2007
Citation: Di, R., Blechl, A.E., Dill-Macky, R., Tortora, A., Tumer, N.E. 2007. Expression of the N-terminal 99 Amino Acids of Yeast Ribosomal Protein L3 in Transgenic Wheat Confers Resistance to Fusarium Head Blight. The 2nd International Conference on Plant Molecular Biology, March 23-27, 2007, Sanya, China. Molecular Plant Breeding. 5:283.

Interpretive Summary:

Technical Abstract: Fusarium head blight (FHB) is a worldwide disease for wheat or barley. The contamination of important agricultural products with the trichothecene mycotoxins such as deoxynivalenol (DON) or 4,15-diacetoxyscirpeno1 (DAS) produced by Fusarium species poses a major health concern for both human and animals. Transgenic wheat plants expressing a truncated form of yeast ribosomal protein L3 (L3A) are produced via particle gun bombardment. Homozygous lines of transgenic wheat plants display resistance to DON when this mycotoxin is incorporated into the medium in seed germination tests. These transgenic plants are shown to be resistant to Fusarium graminearum infection in greenhouse tests accompanied with reduction in the levels of DON on kernels. The up-regulation of the endogenous wheat ribosomal RPL3 gene expression and the transgene expression of L3A are responsible for the enhanced resistance which correlates with our previous observations in transgenic tobacco plants expressing yeast L3A. Our results demonstrate that yeast L3A can be used to control