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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Bioproducts Research » Research » Publications at this Location » Publication #189687
Title: CLONING AND CHARACTERIZATION OF COLD-ACTIVE XYLANASE ENZYMES

Author
item Lee, Charles
item Kibblewhite, Rena
item Wagschal, Kurt
item Robertson, George
item Wong, Dominic

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/16/2005
Publication Date: 4/30/2006
Citation: Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of cold-active xylanase enzymes. 28th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 1A-41.

Interpretive Summary:

Technical Abstract: There is great interest in lignocellulolytic enzymes due to the wide variety of industrial applications for these reagents. At the USDA Agricultural Research Service, we are screening DNA libraries to discover genes encoding new hemicellulolytic enzymes. The libraries were generated both from pure cultures as well as from mixed environmental populations (metagenome). Among the new enzymes isolated are cold-active xylanase enzymes that are categorized as glycosyl hydrolase family 8 and family 10 members. These enzymes have potential applications in processes where the products are heat-sensitive. We describe the cloning, purification, and biochemical characterization of these enzymes.