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Title: ISOLATION AND CHARACTERIZATION OF 149 NOVEL MICROSATELLITE DNA MARKERS FOR STRIPED BASS, MORONE SAXATILIS, AND CROSS-SPECIES AMPLIFICATION IN WHITE BASS, M. CHRYSOPS, AND THEIR HYBRID

Author
item COUCH, CHARLENE - NORTH CAROLINA STATE UNIV
item GARBER, AMBER - NORTH CAROLINA STATE UNIV
item Rexroad, Caird
item ABRAMS, J - NORTH CAROLINA STATE UNVI
item STANNARD, JASON - KENT SEATECH CORPORATION
item WESTERMAN, MARK - KENT SEATECH CORPORATION
item SULLIVAN, CRAIG - NORTH CAROLINA STATE UNIV

Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/1/2005
Publication Date: 9/1/2006
Citation: Couch, C., Garber, A., Rexroad III, C.E., Abrams, J., Stannard, J., Westerman, M., Sullivan, C. 2006. Isolation and characterization of 149 novel microsatellite dna markers for striped bass, morone saxatilis, and cross-species amplification in white bass, m. chrysops, and their hybrid. Molecular Ecology Notes. 6(3):667-669.

Interpretive Summary: Striped bass historically supported large commercial fisheries along the Atlantic coast of North America. Declines in wild fisheries in the mid-1970s created the impetus for aquaculture production of hybrid striped bass (HSB; Morone chrysops × M. saxatilis), which has become the fourth most economically important form of finfish culture in the US. Sustainable production of HSB will require domestication and selective breeding to eliminate dependence on wild broodfish for annual fingerling production and to maximize production efficiency. In an industry-driven initiative in 2002, HSB producers, along with university and government researchers, launched a National Program of Genetic Improvement and Selective Breeding for the HSB Industry. Current resource limitations necessitate a breeding program based on communal rearing of progeny groups for performance testing. We have generated a set of genetic markers which will be utilized in the genetic improvement of striped bass through selective breeding by determining parentage, population structure, and in mapping the genome of this species.

Technical Abstract: Aquaculture of hybrid striped bass (white bass Morone chrysops × striped bass M. saxatilis) is an economically important form of finfish culture; however, growers remain dependent on wild broodstock for annual fingerling production. To support domestication and selective breeding of Morone species, we isolated 153 microsatellite loci from repeat-enriched striped bass DNA libraries. Of these, 147 markers amplified in striped bass (average 4.7 alleles per locus) and 133 amplified in white bass (average 2.2 alleles per locus). Contribution of 149 new microsatellite markers will facilitate pedigree analysis and development of linkage maps for selective breeding of Morone species.