|Fulton, Janet - HY-LINE INTERNATIONAL|
|Ashwell, Christopher - NC STATE UNIVERSITY|
Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: December 6, 2005
Publication Date: January 14, 2006
Citation: Kuo, A., Fulton, J., Clover, C.M., Ashwell, C., McMurtry, J.P. 2006. Chicken microsatellite repeats: Jungle fowl vs commercial birds [abstract]. Plant and Animal Genome. Paper No.P561-241. Technical Abstract: Microsatellites are tandem repeats of short nucleotide sequence motifs. They are very useful for linkage and population genetic studies because of their repeatability and high degree of polymorphism. This study compared the Red Jungle Fowl and commercial layer sequences for newly developed microsatellite markers to identify any changes in microsatellite repeats. Eight hundred-seventy-six microsatellite biomarkers recently identified from the chicken genome sequence were used in this study. These new microsatellites included di-, tri-, tetra- and penta- repeats, with a repeat frequency of 6 or greater. A small subsets of these markers from 6 chromosomal regions were selected for sequencing. Markers were grouped into categories based on the repeat number reported for the Red Jungle Fowl sequence. These categories were then further subdivided into two classes depending on whether the observed allele sizes were similar to the Red Jungle Fowl or different. Sequencing reactions were carried out in both directions and aligned. Preliminary analysis showed differences in allele sizes between Red Jungle Fowl and the commercial layers. Variation was found in the number of repeats, and there were some SNP polymorphisms seen in the flanking regions. All types of repeats, di-, tri-, tetra-, penta-, and complex repeats exhibited considerable polymorphisms between the Jungle Fowl and commercial layers.