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Title: Y15_999FW, A DOMINANT SCAR MARKER LINKED TO THE FUSARIUM WILT RACE 1 (FW) RESISTANCE GENE IN PEA

Author
item Okubara, Patricia
item Keller, Karen
item MCCLENDON, MELISSA - WASHINGTON STATE UNIV.
item INGLIS, DEBRA - WASHINGTON STATE UNIV.
item McPhee, Kevin
item Coyne, Clarice - Clare

Submitted to: Pisum Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/1/2005
Publication Date: 12/1/2005
Citation: Okubara, P.A., Keller, K.E., Mcclendon, M.T., Inglis, D.A., Mcphee, K.E., Coyne, C.J. 2005. Y15_999fw, a dominant scar marker linked to the fusarium wilt race 1 (fw) resistance gene in pea. Pisum Genetics. Vol.37, pp32-35.

Interpretive Summary: Pea is an important rotation crop in the dryland growing regions of the Pacific Northwest. Fusarium wilt poses a significant economic problem in pea-growing regions of the Pacific Northwest and throughout the world. Disease resistance genes provide effective means of controlling Fusarium wilt of pea. Fw, a pea gene conferring complete and specific resistance to the Fusarium wilt race 1 pathogen, has been characterized in previous studies. This report describes a molecular marker or gene “tag”, called Y15_999Fw, which can be used by pea breeders to track the Fw gene in their breeding programs.

Technical Abstract: Fusarium wilt poses a significant economic problem in pea-growing regions of the Pacific Northwest and throughout the world. Fw, a pea gene conferring complete and specific resistance to the Fusarium wilt race 1 pathogen, has been characterized in previous studies. This report describes a 1082 bp SCAR marker called Y15_999Fw which can be used in marker assisted selection. This marker was derived from the RAPD marker Y15_1050, and tested against a mapping population of 80 F8 recombinant inbred lines (RILs). The marker co-segregated with Fw in the resistant parent Green Arrow and 38 RILs that displayed the Green Arrow phenotype at Y15_1050, but was absent in 37 RILs that showed the parental susceptible phenotype at Y15_1050. In addition, these SCAR primers resolved ambiguous RAPD scores in three other RILs. A second Y15 SCAR was found to be monomorphic, and is being used to develop a co-dominant marker for Fw. The Y15_999Fw SCAR will provide breeders with a robust, dominant molecular marker with which to follow the Fw locus in approximately 95% of the progeny.