ISOLATION AND CHARACTERIZATION OF A NOVEL FAMILY (MILD-3) OF MINIATURE INVERTED-REPEAT TRANSPOSABLE ELEMENTS IN THE COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA.

Authors: Yocum, George

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: 5/18/2005
Publication Date: 5/23/2005
Citation: Yocum, G.D. 2005. Isolation and characterization of a novel family (mild-3) of miniature inverted-repeat transposable elements in the Colorado potato beetle, Leptinotarsa decemlineata. Genbank. Accession number: DQ011149.

Interpretive Summary:

Technical Abstract: Using PCR and genomic walking, the sequence of the DAT-3 gene and its flanking regions were determined for a final construct of 3984 bp in length. The gene of DAT-3 is composed of two exons 210 and 148 in bp length separated by a 1107 bp intron. Southern blot experiments demonstrated that there is only a single copy of DAT-3 in the Colorado potato beetle genome. Determining the genomic structure of DAT-3 led to the isolation of three novel families of miniature inverted-repeat transposable elements (Mild-1, -2 and 3). Mild-1a is inserted within the first intron of diapause protein-1. Mild-1a is 284 bp in length, has a 14 bp target site duplication, three sets of subterminal inverted-repeats, is TA rich and can form a hairpin structure. The second element, Mild-2a, is inserted within the 3' terminus of Mild-1a. Mild-2 is 29 bp in length with a 3 bp target site duplication and one set of subterminal inverted-repeats. Mild-3a shares 60% identity with Mild-1a, is 251 bp in length, has a 8 bp target site duplication and has one set of subterminal inverted repeats that form a hairpin structure. The target site duplications of Mild-1 and -3 families share a common conserved core of AATTT. Three truncated members of the Mild-1 family and one truncated member of the Mild-2 family were isolated and provide evidence for a past history of MITE transposition. Accession number: DQ011149