|Williams, J - ROSLIN INSTITUTE|
Submitted to: Germplasm Release
Publication Type: Germplasm Release
Publication Acceptance Date: December 29, 2004
Publication Date: January 1, 2005
Citation: Connor, E.E., Laiakis, E.C., Fernandes, V.M., Williams, J.L. 2005. Sequencing and mapping of the bovine type ii deiodinase (dio2) and type iii deiodinase (dio3) genes. Germplasm Release. Accession numbers AY858551-AY858554. Technical Abstract: Thyroid hormones are critical to normal mammalian development and metabolism. Their activity is regulated in a highly complex, tissue-specific manner by three isoforms of deiodinases. The full-length bovine type II deiodinase (DIO2) and type III deiodinase (DIO3) cDNAs were sequenced revealing a high degree of predicted amino acid sequence identity to their orthologs in other mammalian species and demonstrated the conservation of selenocysteine residues within the catalytic domains of both bovine proteins. In addition, three single nucleotide polymorphisms and one indel were identified in the bovine DIO2 transcript. Bovine DIO2 and DIO3 were positioned on chromosomes 10 and 21, respectively, by radiation hybrid mapping.