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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #170934
Title: SEQUENCE OF THE CANINE MAJOR HISTOCOMPATIBILITY COMPLEX REGION CONTAINING NON-CLASSICAL CLASS 1 GENES

Author
item WAGNER, JOHN - THOMAS JEFFERSON UNI
item Palti, Yniv
item DIDARIO, DONNA - THOMAS JEFFERSON UNI
item FARACO, J - STANFORD UNIVERSITY, PALO

Submitted to: Tissue Antigen
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/9/2005
Publication Date: 6/1/2005
Citation: Wagner, J.L., Palti, Y., Didario, D., Faraco, J. 2005. Sequence of the canine major histocompatibility complex region containing non-classical class 1 genes. Tissue Antigen 65: 549-55.

Interpretive Summary: The canine model has been valuable for hematopoietic stem cell transplantation as well as drug toxicity experiments. Dogs also have a high rate of spontaneous cancers and serve as a model for a variety of cancers including prostate cancer. Understanding the canine major histocompatibility complex (MHC) is important to obtain the maximal benefit from the dog model of human diseases and transplantation as well as understanding diseases in the dog. In this paper we describe the DNA sequence of a segment of 150,102 nucleotides of canine MHC, corresponding to the junction of the class I and class III regions.

Technical Abstract: We have sequenced a segment of 150,102 nucleotides of canine major histocompatibility complex (MHC) DNA, corresponding to the junction of the class I and class III regions. The distal portion contained five class III genes including two tumor necrosis factor genes and the proximal part contained five genes or pseudogenes belonging to the class I region. The order of the class III region genes was conserved as in the porcine and human MHC regions. The order of the class Ib loci from the proximal side outwards was DLA-53, DLA-12a, DLA-64, Stress-induced phosphoprotein-1 (STIP-1), followed by DLA-12. Only DLA-64 and DLA-12 display an overall predicted protein sequence compatible with the expression of membrane-anchored glycoproteins. The other class 1b loci do not appear to be functional by sequence analysis. In all, these 10 genes spanned 24% of the total sequence. The remaining 76% was comprised of a number of noncoding and repetitive DNA elements including long interspersed nuclear elements (LINE) fragments, short interspersed nuclear elements (SINEs), and microsatellites.