|Da Mota, Adilson - EMBRAPA|
|Williams, J - ROSLIN INSTITUTE|
Submitted to: Germplasm Release
Publication Type: Germplasm Release
Publication Acceptance Date: June 18, 2004
Publication Date: June 30, 2004
Citation: Connor, E.E., Sonstegard, T.S., Da Mota, A.F., Wood, D.L., Bennett, G.L., Williams, J.L., Laiakis, E.C., Philpot, J.C., Baumann, R.G., Capuco, A.V. 2004. Genetic mapping and evaluation of estrogen-related receptor alpha (esrra), estrogen receptors alpha (esr1) and beta (esr2) and progesterone receptor (pgr) expression during bovine mammary gland development. Germplasm Release. Accession numbers BV166870-BV166875, AY656812-AY656813. Interpretive Summary: This is a Germplasm Release. No Interpretive Summary Required.
Technical Abstract: Steroid receptors are key transcriptional regulators of mammary growth, development and lactation. Expression of estrogen receptors alpha (ERa) and beta (ERß), progesterone receptor (PR), and estrogen-related receptor alpha-1 (ERRa1) were evaluated in bovine mammary gland obtained from multiple stages of bovine mammary gland development using quantitative real-time RT-PCR. Stages of development included prepubertal heifers, pregnant heifers, lactating non-pregnant cows, lactating pregnant cows and non-lactating pregnant cows. In addition, ERa, ERß, PR, and ERRa1 were mapped to chromosomes 9, 10, 15 and 29, respectively, by linkage and radiation hybrid mapping. Results indicated that expression of ERa, PR, and ERRa1 were largely coordinately regulated and present in significant quantity during all physiological stages evaluated. In contrast, ERß transcripts were present at very low concentration during all stages. Our results also suggest a functional role for ERRa1 and a relative lack of function for ERß in bovine mammary gland development and lactation. Accession Nos. AY656812, AY656813