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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #155070
Title: MAIZE LEAF AXIL MATERIALS AS A SOURCE FOR MONITORING PREVALENCE OF MYCOTOXIGENIC FUSARIUM SPECIES BY PCR ANALYSIS

Author
item Dowd, Patrick
item BARNETT, R - SYNGENTA BIOTECHNOLOGY
item Johnson, Eric
item BECK, J - SYNGENTA BIOTECHNOLOGY

Submitted to: Mycopathologia
Publication Type: Abstract Only
Publication Acceptance Date: 10/15/2003
Publication Date: 6/1/2005
Citation: Dowd, P.F., Barnett, R.J., Johnson, E.T., Beck, J.J. 2003. Maize leaf axil materials as a source for monitoring prevalence of mycotoxigenic fusarium species by pcr analysis. Aflatoxin Elimination, Fumonisin Elimination, and Fungal Genomics Workshops, October 13-15,2003, Savannah, GA. Mycopathologia 158:431-440.

Interpretive Summary:

Technical Abstract: A sampling technique was developed to augment predictions made by a computer program used to forecast mycotoxin presence in Midwest U.S. maize. Samples of different tissues taken from maize plants during 2000-2003, and analyzed using PCR, indicated material that accumulated in leaf axils (primarily anthers and pollen) was a good source of material to detect the presence of Fusarium fungi potentially involved in mycotoxin production. There was considerable variability from field to field and year to year. In both 2002 and 2003, F. proliferatum and F. verticillioides were relatively uncommon compared to F. subglutinans. Visible moldiness of leaf axil material was a relatively good indicator of the presence of Fusarium spp. fungi.