Submitted to: ASA-CSSA-SSSA Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: November 2, 2003
Publication Date: November 20, 2003
Citation: Bae, H., Kim, S., Reddy, V., Sicher Jr, R.C. 2003. Using functional genomics to identify plant genes that respond to carbon dioxide.[abstract]. ASA-CSSA-SSSA Proceedings. P. 308. Technical Abstract: We are interested in determining plant genes affected by plant growth in elevated carbon dioxide. Arabidopsis thaliana plants were raised in growth chambers for six weeks at 36 and 100 Pa carbon dioxide. Total protein extracts were separated by two dimensional electrophoresis. Fourteen proteins were modified by carbon dioxide enrichment and we successfully identified six of these by MALDI TOF mass spectrometry. The six identified polypeptides were the following: 1) myrosinase precursor (63 kD), 2) luminal binding protein 2 (BIP2, 67.8 kD), 3) putative 3 beta hydroxysteroid dehydrogenase/isomerase protein (30.3 kD), 4) nucleoside dikinase II (20.0 kD), 5) major latex protein related (17.5 kD), and 6) photosystem II oxygen evolving complex 23 (OEC23, 23 kD). Carbon dioxide enrichment affected plant genes involved in defense, cellular development, and photosynthesis. The responses of maize genes to carbon dioxide enrichment were determined using 4 to 5 week old plants grown in naturally sunlit SPAR chambers. Patterns of gene expression were compared using maize DNA microarrays. The expression of 33 genes was increased up to 11-fold by carbon dioxide enrichment. At this time, only six of the ESTs discovered by this method have sequence homology to known functional genes. Both DNA microarrays and proteomic analyses can be used to identify plants genes that responded to carbon dioxide enrichment.