|Agrama, Hesham - PLNT PATH NDSU, FARGO, ND|
|Wentz, Mary - PLNT SCI, NDSU, FARGO, ND|
|Steffenson, Brian - PLNT PATH, UOM ST PAUL,MN|
Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: July 15, 2003
Publication Date: November 5, 2003
Citation: Agrama, H., Wentz, M., Dahleen, L.S., Jin, Y., Steffenson, B. 2003. Crown rust resistance gene rpc1 maps to barley chromosome 3H. Agronomy Abstracts. In Annual Meeting Abstracts [CD-ROM] ASA, CSSA, SSSA, Madison, WI. Technical Abstract: Crown rust, Puccinia coronata var. hordei, is an important disease of barley in the Great Plains region of the United States and Canada. Bulked segregant analysis was used to rapidly identify random amplified polymorphic DNA (RAPD) markers linked to the P. coronata resistance gene Rpc1. F2 and F3 populations developed from a cross between the resistant line Hor2596 (CIho 1243) and the susceptible line Bowman were used in this study. A total of 15 RAPDs, 62 microsatellite markers (SSRs) and 32 restriction fragment length polymorphisms (RFLPs) were polymorphic between the resistant and susceptible parents and were applied to map Rcp1. These polymorphic markers were applied to screen 97 F2 individuals and F3 families, and used to identify their linkage to Rpc1 using the Map Manager software package. Two RFLP and five SSR markers on long arm of chromosome 3H, and one RAPD marker were found to be linked with Rpc1, and used to construct a 30 cM linkage map containing Rpc1 locus. The genetic distance between Rpc1 and the closest RAPD marker O8_700 was 2.5 cM. The linked markers will be used to incorporate the crown rust resistance gene into barley breeding lines.