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Title: DETECTION OF DNA POLYMORPHISMS IN GLASSY-WINGED SHARPSHOOTERS (HOMALODISCA COAGULATA) BY PCR-BASED DNA FINGERPRINTING METHODS

Author
item De Leon, Jesus
item Jones, Walker
item MORGAN, DAVID - CA.DEPT. OF FOOD & AG

Submitted to: CDFA Pierce's Disease Control Program Research Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 10/20/2003
Publication Date: 12/8/2003
Citation: De Leon, J.H., Jones, W.A., Morgan, D.J. 2003. Detection of DNA polymorphisms in glassy-winged sharpshooters (Homalodisca coagulata) by PCR-based DNA fingerprinting methods [abstract]. In: Proceedings of the CDFA Pierce's Disease Control Program Research Symposium. Dec. 15-18, 2002, San Diego, CA. p. 171-172.

Interpretive Summary:

Technical Abstract: The glassy-winged sharpshooter Homalodisca coagulata (Say) (Homoptera: Cicadellidae), is a xylem feeding leafhopper that is a serious pest because it vectors a strain of Xylella fastidiosa, a bacterium that causes Pierce's Disease of grapevines (Turner and Pollard, 1959; Nielsen, 1968). DNA markers have proved to be valuable tools for population genetic studies. DNA fingerprinting methods that do not require prior knowledge of genome sequences include ISSR-PCR (Inter-Simple Sequence Repeat-Polymerase Chain Reaction), RAMP (Randomly Amplified Microsatellite Polymorphisms), SAMPL (Selective Amplification of Microsatellite Polymorphic Loci) and RAPD (Random Amplification of Polymorphic DNA). RAPDs produce dominant markers, whereas ISSR-PCR, RAMP, and SAMPL incorporate Simple Sequence Repeats (SSR) and are capable of identifying co-dominant markers if utilizing 5'-anchored or compound ISSR primers (reviewed in Karp and Edwards, 1997), but without known family relationships (segregation/backcrosses) these markers are scored as dominant.