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Title: THE MAIZE GENOME: A CHALLENGE MET

Author
item Coe Jr, Edward
item CONE, KAREN - UNIV OF MISSOURI
item SANCHEZ-VILLEDA, HECTOR - UNIV OF MISSOURI
item SCHROEDER, STEVE - UNIV OF MISSOURI
item FANG, ZHIWEI - UNIV OF MISSOURI
item Schaeffer, Mary
item GARDINER, JACK - UNIV OF MISSOURI
item McMullen, Michael
item VROH BI, IRIE - UNIV OF MISSOURI
item KIM, YOUNG-SUN - UNIV OF MISSOURI
item WING, RODNEY - UNIV OF ARIZONA
item SODERLUND, CAROL - UNIV OF ARIZONA
item BUTLER, EDWARD - UNIV OF ARIZONA
item GOICOECHEA, JOSE LUIS - UNIV OF ARIZONA
item ENGLER, FRED - UNIV OF ARIZONA
item PATERSON, ANDREW - UNIV OF GEORGIA-ATHENS
item BOWERS, JOHN - UNIV OF GEORGIA-ATHENS

Submitted to: International Congress of Genetics Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 2/26/2003
Publication Date: 7/6/2003
Citation: COE JR, E.H., CONE, K., SANCHEZ-VILLEDA, H., SCHROEDER, S., FANG, Z., POLACCO, M.L., GARDINER, J., MCMULLEN, M.D., VROH BI, I., KIM, Y., WING, R., SODERLUND, C., BUTLER, E., GOICOECHEA, J., ENGLER, F., PATERSON, A., BOWERS, J.E. THE MAIZE GENOME: A CHALLENGE MET. INTERNATIONAL CONGRESS OF GENETICS PROCEEDINGS. 2003. Abstract. p. 234.

Interpretive Summary:

Technical Abstract: The genetic/physical maps of the Maize Mapping Project are ready for research studies and for sequencing. The raw question, "can this genome be assembled, given that it is highly duplicated and is rife with recently diverged, nested retrotransposons?" has been answered in the affirmative. The maize genome is as large as the human genome, and equally, if not more, complex. BACs (27X) from HindIII, EcoRI, and Mbol libraries of B73 inbred line gave 290,000 usable fingerprints (15X coverage) that have been assembled into contigs using FPC. The January 2003 build drew together 4,518 phase I contigs assembled at a cutoff of 10e-12. In Phase II, manual editing is advancing rapidly with the help of an end-joining utility and over 13,000 markers, including 9,300 unigenes addressed to the BACs with 40bp overgos. Precise addressing of mapped markers to BACs is done in pools by PCR. Contig order and orientation devolves from over 2000 markers on a high-resolution mapping population. SNP mapping is focused on EST unigenes that are associated with single contigs. Intercalation of loci from other informative populations into a "neighbors" map will provide over 8,000 mapped loci. Editing of contigs should be complete by Summer 2003. Software tools that permit us to systematize, to validate, to link, and to integrate the large, diverse, high-throughput datasets are available. See http://www.maizemap.org/ and http://genome.arizona.edu/fpc/maize/ for views and downloads. Side-by-side dynamic views of the physical and genetic map are accessible from a database environment in the Integrated Map (iMap).