|Brummer, E - IOWA STATE UNIVERSITY|
|Luth, D - I0WA STAT UNIVERSITY|
Submitted to: American Society of Agronomy
Publication Type: Abstract Only
Publication Acceptance Date: July 25, 2003
Publication Date: July 25, 2003
Citation: Campbell, T.A., Brummer, E.C., Luth, D., Bauchan, G.R., Xia, Z., He, C.N. 2003. Screening autotetraploid alfalfa and medicago truncatula SSR makers for inclusion in a cultivated alfalfa linkage map [abstract]. International Symposium on Molecular Breeding of Forage Crops Proceedings. P927. Technical Abstract: This research was designed to identify simple sequence repeat (SSR or microsatellite) DNA markers for integration into an autotetraploid alfalfa (Medicago sativa L.) linkage map. Up to eight SSR markers can be mapped per locus and the resulting 32 linkage groups should ultimately coalesce into eight. Markers associated with agronomically important quantitative and qualitative genes in the mapping population will be identified for use in marker assisted selection, and marker polymorphisms will be used to evaluate diversity and phylogenic relationships in Medicago species. Tri- and tetra-nucleotide SSR markers from tetraploid alfalfa genomic libraries and from ESTs and BAC libraries of the closely related diploid M. truncatula will be given priority. So far, 575 primer pairs have been screened and marker patterns for 259 pairs indicate a single-dose response for one or more alleles and indicate that these alleles can probably be mapped. Forty-two primer pairs trace to SSRs discovered in an autotetraploid alfalfa genomic library by C. He; the remainder trace to SSRs discovered in M. truncatula ESTs by T. A. Campbell, T. Huguet (CNRS-INRA, Castanet-Tolosan, France), M. A. Rouf Mian, (The Noble Foundation, Ardmore Oklahoma, USA), and N. D. Young (University of Minnesota, St. Paul Minnesota, USA). Promising primer pairs will be retested on F1 s, allelic marker patterns will be analyzed for conformation to a 1:1 ratio, and conforming alleles will be mapped.